1887

Abstract

A bacterial isolate, designated strain D5, was isolated from a soil sample collected from the Inner Mongolia Autonomous Region, China, and subjected to a taxonomic investigation using a polyphasic approach. Strain D5 was aerobic, Gram-stain-negative, rod-shaped and motile. Strain D5 fell within the evolutionary radius of the genus in the phylogenetic tree based on 16S rRNA gene sequences and was most closely related to 76 with 97.3 % 16S rRNA gene sequence similarity. The predominant quinone of strain D5 was Q-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were summed feature 3 (comprising Cω7 and/or iso-C 2-OH) and C. These chemotaxonomic data supported the affiliation of strain D5 to the genus . The genomic DNA G+C content was 65.9 mol%. Mean DNA–DNA relatedness values between strain D5 and the phylogenetically most closely related species of the genus , KCTC 12344 and KCTC 12342, were 26 and 21 %, respectively. Strain D5 could be differentiated from recognized species of the genus by several phenotypic characteristics. It is clear from the data presented that strain D5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is D5 ( = CGMCC 1.10822 = KCTC 23880).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.047068-0
2013-06-01
2020-01-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/6/2118.html?itemId=/content/journal/ijsem/10.1099/ijs.0.047068-0&mimeType=html&fmt=ahah

References

  1. Collins M. D., Jones D.. ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Bacteriol 48:, 459–470. [CrossRef]
    [Google Scholar]
  2. Collins M. D., Howarth O. W., Grund E., Kroppenstedt R. M.. ( 1987;). Isolation and structural determination of new members of the vitamin K2 series in Nocardia brasiliensis. . FEMS Microbiol Lett 41:, 35–39. [CrossRef]
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  4. Du Y., Yu X., Wang G.. ( 2012;). Massilia tieshanensis sp. nov., isolated from mining soil. . Int J Syst Evol Microbiol 62:, 2356–2362. [CrossRef][PubMed]
    [Google Scholar]
  5. Gallego V., Sánchez-Porro C., García M. T., Ventosa A.. ( 2006;). Massilia aurea sp. nov., isolated from drinking water. . Int J Syst Evol Microbiol 56:, 2449–2453. [CrossRef][PubMed]
    [Google Scholar]
  6. Huβ V. A., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef][PubMed]
    [Google Scholar]
  7. Kämpfer P., Kroppenstedt R. M.. ( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  8. Kämpfer P., Falsen E., Busse H.-J.. ( 2008;). Naxibacter varians sp. nov. and Naxibacter haematophilus sp. nov., and emended description of the genus Naxibacter. . Int J Syst Evol Microbiol 58:, 1680–1684. [CrossRef][PubMed]
    [Google Scholar]
  9. Kämpfer P., Lodders N., Martin K., Falsen E.. ( 2011;). Revision of the genus Massilia La Scola et al. 2000, with an emended description of the genus and inclusion of all species of the genus Naxibacter as new combinations, and proposal of Massilia consociata sp. nov.. Int J Syst Evol Microbiol 61:, 1528–1533. [CrossRef][PubMed]
    [Google Scholar]
  10. Kämpfer P., Lodders N., Martin K., Falsen E.. ( 2012;). Massilia oculi sp. nov., isolated from a human clinical specimen. . Int J Syst Evol Microbiol 62:, 364–369. [CrossRef][PubMed]
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  12. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  13. Kluge A. G., Farris F. S.. ( 1969;). Quantitative phyletics and the evolution of anurans. . Syst Zool 18:, 1–32. [CrossRef]
    [Google Scholar]
  14. Kong B.-H., Li Y.-H., Liu M., Liu Y., Li C.-L., Liu L., Yang Z.-W., Yu R.. ( 2013;). Massilia namucuonensis sp. nov., isolated from a soil sample. . Int J Syst Evol Microbiol 63:, 352–357. [CrossRef][PubMed]
    [Google Scholar]
  15. La Scola B., Birtles R. J., Mallet M. N., Raoult D.. ( 1998;). Massilia timonae gen. nov., sp. nov., isolated from blood of an immunocompromised patient with cerebellar lesions. . J Clin Microbiol 36:, 2847–2852.[PubMed]
    [Google Scholar]
  16. Lindquist D., Murrill D., Burran W. P., Winans G., Janda J. M., Probert W.. ( 2003;). Characteristics of Massilia timonae and Massilia timonae-like isolates from human patients, with an emended description of the species. . J Clin Microbiol 41:, 192–196. [CrossRef][PubMed]
    [Google Scholar]
  17. Mackie T. J., McCartney J. E.. ( 1989;). Microscopy. In Mackie and McCartney's Practical Medical Microbiology, , 13th edn., pp. 11–37. Edited by Collee J. G., Duguid J. P., Fraser A. G., Marmion B. P... London:: Churchill Livingstone;.
    [Google Scholar]
  18. Marmur J., Doty P.. ( 1962;). Determination of base composition of deoxyribonucleic acid from its denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef][PubMed]
    [Google Scholar]
  19. Minnikin D. E., Collins M. D., Goodfellow M.. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95. [CrossRef]
    [Google Scholar]
  20. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E.. ( 1996;). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsiaceae fam. nov.. Int J Syst Bacteriol 46:, 1088–1092. [CrossRef][PubMed]
    [Google Scholar]
  21. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  22. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  23. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  24. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  26. Wang J.-W., Zhang J.-L., Pang H., Zhang Y.-B., Li Y.-Y., Fan J.-P.. ( 2012;). Massilia flava sp. nov., isolated from soil. . Int J Syst Evol Microbiol 62:, 580–585. [CrossRef][PubMed]
    [Google Scholar]
  27. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  28. Weon H. Y., Kim B. Y., Son J. A., Jang H. B., Hong S. K., Go S. J., Kwon S. W.. ( 2008;). Massilia aerilata sp. nov., isolated from an air sample. . Int J Syst Evol Microbiol 58:, 1422–1425. [CrossRef][PubMed]
    [Google Scholar]
  29. Weon H. Y., Kim B. Y., Hong S. B., Jeon Y. A., Koo B. S., Kwon S. W., Stackebrandt E.. ( 2009;). Massilia niabensis sp. nov., and Massilia niastensis sp. nov., isolated from air samples. . Int J Syst Evol Microbiol 59:, 1656–1660. [CrossRef][PubMed]
    [Google Scholar]
  30. Weon H. Y., Yoo S. H., Kim S. J., Kim Y. S., Anandham R., Kwon S. W.. ( 2010;). Massilia jejuensis sp. nov. and Naxibacter suwonensis sp. nov., isolated from air samples. . Int J Syst Evol Microbiol 60:, 1938–1943. [CrossRef][PubMed]
    [Google Scholar]
  31. Wu C., Lu X., Qin M., Wang Y., Ruan J.. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16:, 176–178.
    [Google Scholar]
  32. Xu P., Li W.-J., Tang S.-K., Zhang Y.-Q., Chen G.-Z., Chen H.-H., Xu L.-H., Jiang C.-L.. ( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55:, 1149–1153. [CrossRef][PubMed]
    [Google Scholar]
  33. Yoon J.-H., Lee S. T., Park Y.-H.. ( 1998;). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. . Int J Syst Bacteriol 48:, 187–194. [CrossRef][PubMed]
    [Google Scholar]
  34. Yoon J.-H., Kang K.-H., Park Y.-H.. ( 2003;). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 53:, 449–454. [CrossRef][PubMed]
    [Google Scholar]
  35. Zhang Y.-Q., Li W.-J., Zhang K.-Y., Tian X.-P., Jiang Y., Xu L.-H., Jiang C.-L., Lai R.. ( 2006;). Massilia dura sp. nov., Massilia albidiflava sp. nov., Massilia plicata sp. nov. and Massilia lutea sp. nov., isolated from soils in China. . Int J Syst Evol Microbiol 56:, 459–463. [CrossRef][PubMed]
    [Google Scholar]
  36. Zul D., Wanner G., Overmann J.. ( 2008;). Massilia brevitalea sp. nov., a novel betaproteobacterium isolated from lysimeter soil. . Int J Syst Evol Microbiol 58:, 1245–1251. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.047068-0
Loading
/content/journal/ijsem/10.1099/ijs.0.047068-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error