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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_7

sp. nov., isolated from subarctic timothy grass ( L.) silage Free

Abstract

Four strains of Gram-positive, non-spore-forming, rod-shaped, catalase-negative and non-motile lactic acid bacteria, LOOC260, LOOC253, LOOC273 and LOOC279, were isolated from timothy grass ( L.) silage produced in Hokkaido, a subarctic region of Japan. These isolates grew at 4–37 °C, indicating the psychrotolerant nature of these strains. Phylogenetic analysis on the basis of 16S rRNA and gene sequences, as well as biochemical and physiological characteristics, indicated that these four strains were members of the genus . 16S rRNA gene sequence analysis of strain LOOC260 demonstrated that the closest neighbours were the type strains of (97.7 %), (96.7 %) and (96.7 %). Strain LOOC260 showed low levels of DNA–DNA association with JCM 9504 (14.7±3.5 %), JCM 16175 (15.1±4.8 %) and JCM 1716 (10.7±3.0 %). The cell wall contained -diaminopimelic acid and the major fatty acids were Cω9 and C cyclo 9,10. On the basis of phenotypic, physiological and phylogenetic evidence, these isolates represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LOOC260 ( = JCM 18461 = DSM 26202).

  • Published Online:
Funding
This study was supported by the:
  • Ministry of Agriculture, Forestry and Fisheries of Japan
© 2013 IUMS
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2013-07-01
2024-04-25
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Lactobacillushokkaidonensis sp. nov., isolated from subarctic timothy grass (Phleum pratense L.) silage
Int J Syst Evol Microbiol 63, 2526 (2013); https://doi.org/10.1099/ijs.0.047027-0
/content/journal/ijsem/10.1099/ijs.0.047027-0
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