1887

Abstract

Four strains of Gram-positive, non-spore-forming, rod-shaped, catalase-negative and non-motile lactic acid bacteria, LOOC260, LOOC253, LOOC273 and LOOC279, were isolated from timothy grass ( L.) silage produced in Hokkaido, a subarctic region of Japan. These isolates grew at 4–37 °C, indicating the psychrotolerant nature of these strains. Phylogenetic analysis on the basis of 16S rRNA and gene sequences, as well as biochemical and physiological characteristics, indicated that these four strains were members of the genus . 16S rRNA gene sequence analysis of strain LOOC260 demonstrated that the closest neighbours were the type strains of (97.7 %), (96.7 %) and (96.7 %). Strain LOOC260 showed low levels of DNA–DNA association with JCM 9504 (14.7±3.5 %), JCM 16175 (15.1±4.8 %) and JCM 1716 (10.7±3.0 %). The cell wall contained -diaminopimelic acid and the major fatty acids were Cω9 and C cyclo 9,10. On the basis of phenotypic, physiological and phylogenetic evidence, these isolates represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LOOC260 ( = JCM 18461 = DSM 26202).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.047027-0
2013-07-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/7/2526.html?itemId=/content/journal/ijsem/10.1099/ijs.0.047027-0&mimeType=html&fmt=ahah

References

  1. Cai Y., Pang H., Kitahara M., Ohkuma M.. ( 2012;). Lactobacillus nasuensis sp. nov., a lactic acid bacterium isolated from silage, and emended description of the genus Lactobacillus. . Int J Syst Evol Microbiol 62:, 1140–1144. [CrossRef][PubMed]
    [Google Scholar]
  2. Cousin S., Gulat-Okalla M. L., Motreff L., Gouyette C., Bouchier C., Clermont D., Bizet C.. ( 2012;). Lactobacillus gigeriorum sp. nov., isolated from chicken crop. . Int J Syst Evol Microbiol 62:, 330–334. [CrossRef][PubMed]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane-filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  4. Hitchener B. J., Egan A. F., Rogers P. J.. ( 1982;). Characteristics of lactic acid bacteria isolated from vacuum-packaged beef. . J Appl Bacteriol 52:, 31–37. [CrossRef][PubMed]
    [Google Scholar]
  5. Irisawa T., Okada S.. ( 2009;). Lactobacillus sucicola sp. nov., a motile lactic acid bacterium isolated from oak tree (Quercus sp.) sap. . Int J Syst Evol Microbiol 59:, 2662–2665. [CrossRef][PubMed]
    [Google Scholar]
  6. Kaksonen A. H., Spring S., Schumann P., Kroppenstedt R. M., Puhakka J. A.. ( 2008;). Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal- and sulfate-containing wastewater. . Int J Syst Evol Microbiol 58:, 833–838. [CrossRef][PubMed]
    [Google Scholar]
  7. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  8. Kitahara M., Sakamoto M., Benno Y.. ( 2010;). Lactobacillus similis sp. nov., isolated from fermented cane molasses. . Int J Syst Evol Microbiol 60:, 187–190. [CrossRef][PubMed]
    [Google Scholar]
  9. Kleynmans U., Heinzl H., Hammes W. P.. ( 1989;). Lactobacillus suebicus sp. nov., an obligately heterofermentative Lactobacillus species isolated from fruit mashes. . Syst Appl Microbiol 11:, 267–271. [CrossRef]
    [Google Scholar]
  10. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Method Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  11. Koort J., Murros A., Coenye T., Eerola S., Vandamme P., Sukura A., Björkroth J.. ( 2005;). Lactobacillus oligofermentans sp. nov., associated with spoilage of modified-atmosphere-packaged poultry products. . Appl Environ Microbiol 71:, 4400–4406. [CrossRef][PubMed]
    [Google Scholar]
  12. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A.. & other authors ( 2007;). clustal w and clustal_x version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef][PubMed]
    [Google Scholar]
  13. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J.. ( 2005;). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. . Microbiology 151:, 2141–2150. [CrossRef][PubMed]
    [Google Scholar]
  14. Naser S. M., Dawyndt P., Hoste B., Gevers D., Vandemeulebroecke K., Cleenwerck I., Vancanneyt M., Swings J.. ( 2007;). Identification of lactobacilli by pheS and rpoA gene sequence analyses. . Int J Syst Evol Microbiol 57:, 2777–2789. [CrossRef][PubMed]
    [Google Scholar]
  15. Okada S., Suzuki Y., Kozaki M.. ( 1979;). New heterofermentative Lactobacillus species with meso-diaminopimelic acid in peptidoglycan, Lactobacillus vaccinostercus Kozaki and Okada sp. nov.. J Gen Appl Microbiol 25:, 215–221. [CrossRef]
    [Google Scholar]
  16. Oki K., Kudo Y., Watanabe K.. ( 2012;). Lactobacillus saniviri sp. nov. and Lactobacillus senioris sp. nov., isolated from human faeces. . Int J Syst Evol Microbiol 62:, 601–607. [CrossRef][PubMed]
    [Google Scholar]
  17. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  18. Sakamoto M., Suzuki M., Umeda M., Ishikawa I., Benno Y.. ( 2002;). Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov.. Int J Syst Evol Microbiol 52:, 841–849. [CrossRef][PubMed]
    [Google Scholar]
  19. Suzuki K., Sasaki J., Uramoto M., Nakase T., Komagata K.. ( 1996;). Agromyces mediolanus sp. nov., nom. rev., comb. nov., a species for “Corynebacterium mediolanum” Mamoli 1939 and for some aniline-assimilating bacteria which contain 2,4-diaminobutyric acid in the cell wall peptidoglycan. . Int J Syst Bacteriol 46:, 88–93. [CrossRef][PubMed]
    [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  21. Tohno M., Kitahara M., Inoue H., Uegaki R., Irisawa T., Ohkuma M., Tajima K.. ( 2012a;). Weissella oryzae sp. nov., isolated from fermented rice grain (Oryza sativa L. subsp. japonica). . Int J Syst Evol Microbiol. 63:, 1417–1420. [CrossRef][PubMed]
    [Google Scholar]
  22. Tohno M., Kobayashi H., Nomura M., Kitahara M., Ohkuma M., Uegaki R., Cai Y.. ( 2012b;). Genotypic and phenotypic characterization of lactic acid bacteria isolated from Italian ryegrass silage. . Anim Sci J 83:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  23. Tohno M., Kobayashi H., Nomura M., Uegaki R., Cai Y.. ( 2012c;). Identification and characterization of lactic acid bacteria isolated from mixed pasture of timothy and orchardgrass, and its badly preserved silage. . Anim Sci J 83:, 318–330. [CrossRef][PubMed]
    [Google Scholar]
  24. Tohno M., Kobayashi H., Tajima K., Uegaki R.. ( 2012d;). Strain-dependent effects of inoculation of Lactobacillus plantarum subsp. plantarum on fermentation quality of paddy rice (Oryza sativa L. subsp. japonica) silage. . FEMS Microbiol Lett 337:, 112–119. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.047027-0
Loading
/content/journal/ijsem/10.1099/ijs.0.047027-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error