1887

Abstract

A Gram-reaction-positive, facultatively anaerobic, motile, endospore-forming, rod-shaped strain, designated SgZ-7, was isolated from a windrow compost pile and was characterized by means of a polyphasic approach. Growth occurred with 0–3 % (w/v) NaCl (optimum 1 %), at pH 6.0–10.0 (optimum pH 7.2) and at 40–60 °C (optimum 50 °C). The main respiratory quinone was MK-7. The predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were iso-C and anteiso-C. The DNA G+C content was 46.6 mol%. The phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain SgZ-7 should be assigned to the genus and was related most closely to LMG 21831 (sequence similarity 97.2 %). The result of the DNA–DNA hybridization experiment revealed a low relatedness (27.2 %) between the isolate and LMG 21831. The results of phenotypic, chemotaxonomic and genotypic analyses clearly indicated that strain SgZ-7 represents a novel species, for which the name sp. nov. is proposed. The type strain is SgZ-7 ( = CCTCC AB 2012030 = KACC 16700).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.046953-0
2013-08-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/8/3024.html?itemId=/content/journal/ijsem/10.1099/ijs.0.046953-0&mimeType=html&fmt=ahah

References

  1. Cohn F.. ( 1872;). Genus Micrococcus. . Beitr Biol Pflanz 1:, 127–224.
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef][PubMed]
    [Google Scholar]
  3. Doetsch R. N.. ( 1981;). Determinative methods of light microscopy. . In Mannual of Methods for General Bacteriology, pp. 21–33. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Philips G. B... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  4. Dong X., Cai M.. ( 2001;). Manual of Systematic and Determinative Bacteriology. Beijing, China:: Academic Press;.
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane-filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  6. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  7. Ghosh J., Larsson P., Singh B., Pettersson B. M. F., Islam N. M., Sarkar S. N., Dasgupta S., Kirsebom L. A.. ( 2009;). Sporulation in mycobacteria. . Proc Natl Acad Sci U S A 106:, 10781–10786. [CrossRef][PubMed]
    [Google Scholar]
  8. Heyrman J., Vanparys B., Logan N. A., Balcaen A., Rodríguez-Díaz M., Felske A., De Vos P.. ( 2004;). Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands. . Int J Syst Evol Microbiol 54:, 47–57. [CrossRef][PubMed]
    [Google Scholar]
  9. Kämpfer P.. ( 1994;). Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species. . Syst Appl Microbiol 17:, 86–98. [CrossRef][PubMed]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  11. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  12. Leifson E.. ( 1963;). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85:, 1183–1184.[PubMed]
    [Google Scholar]
  13. Logan N. A., De Vos P.. ( 2009;). Genus Bacillus Cohn 1872. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 21–127. Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  14. Logan N. A., Lebbe L., Hoste B., Goris J., Forsyth G., Heyndrickx M., Murray B. L., Syme N., Wynn-Williams D. D., De Vos P.. ( 2000;). Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov.. Int J Syst Evol Microbiol 50:, 1741–1753.[PubMed]
    [Google Scholar]
  15. Ma C., Zhuang L., Zhou S. G., Yang G. Q., Yuan Y., Xu R. X.. ( 2012;). Alkaline extracellular reduction: isolation and characterization of an alkaliphilic and halotolerant bacterium, Bacillus pseudofirmus MC02. . J Appl Microbiol 112:, 883–891. [CrossRef][PubMed]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrate procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  18. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101. Newark, Delaware:: Microbial ID;.
    [Google Scholar]
  19. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S.. ( 2001;). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. Int J Syst Evol Microbiol 51:, 1639–1652. [CrossRef][PubMed]
    [Google Scholar]
  20. Tamaoka J., Katayamafujimura Y., Kuraishi H.. ( 1983;). Analysis of bacterial menaquinone mixtures by high-performance liquid-chromatography. . J Appl Bacteriol 54:, 31–36. [CrossRef]
    [Google Scholar]
  21. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  22. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  23. Vaishampayan P., Probst A., Krishnamurthi S., Ghosh S., Osman S., McDowall A., Ruckmani A., Mayilraj S., Venkateswaran K.. ( 2010;). Bacillus horneckiae sp. nov., isolated from a spacecraft-assembly clean room. . Int J Syst Evol Microbiol 60:, 1031–1037. [CrossRef][PubMed]
    [Google Scholar]
  24. Vaz-Moreira I., Figueira V., Lopes A. R., Lobo-da-Cunha A., Spröer C., Schumann P., Nunes O. C., Manaia C. M.. ( 2012;). Bacillus purgationiresistans sp. nov., isolated from a drinking-water treatment plant. . Int J Syst Evol Microbiol 62:, 71–77. [CrossRef][PubMed]
    [Google Scholar]
  25. Wayne L., Brenner D., Colwell R., Grimont P., Kandler O., Krichevsky M., Moore L., Moore W., Murray R.. & other authors ( 1987;). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  26. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
  27. Zhang L., Wang Y., Dai J., Tang Y. L., Yang Q., Luo X. S., Fang C. X.. ( 2009;). Bacillus korlensis sp. nov., a moderately halotolerant bacterium isolated from a sand soil sample in China. . Int J Syst Evol Microbiol 59:, 1787–1792. [CrossRef][PubMed]
    [Google Scholar]
  28. Zhang J. L., Wang J. W., Fang C. Y., Song F., Xin Y. H., Qu L., Ding K.. ( 2010;). Bacillus oceanisediminis sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 60:, 2924–2929. [CrossRef][PubMed]
    [Google Scholar]
  29. Zhang L., Wu G. L., Wang Y., Dai J., Fang C. X.. ( 2011;). Bacillus deserti sp. nov., a novel bacterium isolated from the desert of Xinjiang, China. . Antonie van Leeuwenhoek 99:, 221–229. [CrossRef][PubMed]
    [Google Scholar]
  30. Zhang Y. Z., Chen W. F., Li M., Sui X. H., Liu H. C., Zhang X. X., Chen W. X.. ( 2012;). Bacillus endoradicis sp. nov., an endophytic bacterium isolated from soybean root. . Int J Syst Evol Microbiol 62:, 359–363. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.046953-0
Loading
/content/journal/ijsem/10.1099/ijs.0.046953-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error