A thermophilic, anaerobic, chemolithoautotrophic bacterium (strain S69) was isolated from a deep-sea hydrothermal vent chimney located on the Eastern Lau Spreading Center and Valu Fa Ridge, Pacific Ocean, at a depth of 1910 m using anoxic medium with elemental sulfur as the only energy source. Cells of strain S69 were Gram-negative short rods, 0.4–0.6 µm in diameter and 1.0–2.5 µm in length, motile with a single polar flagellum. The temperature range for growth was 28–70 °C, with an optimum at 61 °C. The pH range for growth was 5.6–7.9, with optimum growth at pH 6.8. Growth of strain S69 was observed at NaCl concentrations ranging from 0.9 to 5.0 %, with an optimum at 1.8–2.7 (w/v). Strain S69 grew anaerobically with elemental sulfur as an energy source and bicarbonate/CO as a carbon source. Elemental sulfur was disproportionated to sulfide and sulfate. Growth was enhanced in the presence of poorly crystalline Fe(III) oxide (ferrihydrite) as a sulfide-scavenging agent. Strain S69 was also able to grow by disproportionation of thiosulfate and sulfite. Sulfate was not used as an electron acceptor either with H or with organic electron donors. Analysis of the 16S rRNA gene sequence revealed that the isolate formed a distinct phylogenetic branch within the . On the basis of its physiological properties and results of phylogenetic analyses, strain S69 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is S69 ( = DSM 25762 = VKM B-2760).

This study was supported by the:
  • Russian Foundation for Basic Research (Award 12-04-00789-a)
  • Russian Academy of Sciences
  • United States National Science Foundation (Award OCE-0937404, OCE-0752469 and OCE-0728391)

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