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Volume 63, Issue Pt_7

sp. nov., a nitrogen-fixing bacterium isolated from a microbial fuel cell Free

Abstract

A Gram-negative, facultative anaerobic, motile, spiral, straight-to-slightly curved rod-shaped and nitrogen-fixing strain, designated SgZ-5, was isolated from a microbial fuel cell (MFC) and was characterized by means of a polyphasic approach. Growth occurred with 0–1 % (w/v) NaCl (optimum 1 %) and at pH 5.5–8.5 (optimum pH 7.2) and at 25–37 °C (optimum 30 °C) in nutrient broth (NB). The strain had the ability to grow under anaerobic conditions via the oxidation of various organic compounds coupled to the reduction of anthraquione-2,6-disulfonate (AQDS). Chemotaxonomic characteristics (main ubiquinone Q-10, major fatty acid Cω7/Cω6 and DNA G+C content 67.7 mol%) were similar to those of members of the genus . According to the results of phylogenetic analyses, strain SgZ-5 belonged to the genus within the family of the class , and was related most closely to the type strains of , and (98.0, 97.6 and 97.1 % 16S rRNA gene sequence similarity, respectively). DNA–DNA pairing studies showed that the unidentified organism displayed reassociation values of 36.7±3.7, 24.1±2.2 and 22.3±2.4 % to the type strains of , and , respectively. Similarities between gene sequences of strain SgZ-5 and members of the genus ranged from 94.0 to 97.0 %. A combination of phenotypic, chemotaxonomic, phylogenetic and genotypic data clearly indicated that strain SgZ-5 represents a novel species, for which the name sp. nov. is proposed. The type strain is SgZ-5 ( = CCTCC AB 2012021 = KACC 16605).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41171205, 21177030 and 41071157)
  • National Key Technology R&D Program of China (Award 2012BAD14B16)
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2013-07-01
2024-04-24
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Azospirillum humicireducens sp. nov., a nitrogen-fixing bacterium isolated from a microbial fuel cell
Int J Syst Evol Microbiol 63, 2618 (2013); https://doi.org/10.1099/ijs.0.046813-0
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