1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_6

gen. nov., sp. nov., a prosthecate alphaproteobacterium of the family isolated from deep seawater Free

Abstract

A marine prosthecate bacterium, designated strain P-1 km-3, was isolated from deep seawater from the Pacific. Cells of strain P-1 km-3 were Gram-stain-negative, aerobic, catalase- and oxidase-positive, dimorphic rods with a single polar prostheca or flagellum. The strain hydrolysed gelatin and grew at 6–40 °C (optimum, 30 °C) and with 0.5–12 % (w/v) NaCl (optimum, 2 %). Phylogenetic analysis of the 16S rRNA gene sequences revealed that strain P-1 km-3 belonged to the family in the class and represented a separate lineage, located between the genera and . Sequence similarities of strain P-1 km-3 with type strains of species of the genera and were 93.2–93.9 %. The predominant cellular fatty acids in strain P-1 km-3 were Cω7, C, 11-methyl Cω7, C and C cyclo ω8. The major respiratory quinone of strain P-1 km-3 was Q-10. The polar lipids of strain P-1 km-3 comprised glucuronopyranosyldiglyceride (GUDG), monoglycosyldiglyceride (MGDG), sulfo-quinovosyl diacylglycerol (SQDG), phosphatidylglycerol (PG), an unidentified phospholipid (PL) and an unidentified lipid (L). The genomic DNA G+C content of strain P-1 km-3 was 66.0 mol%. On the basis of the polyphasic data presented in this study, strain P-1 km-3 is proposed to represent a novel species in a new genus, gen. nov., sp. nov., within the family . The type strain of the type species is P-1 km-3 ( = KACC 16526 = CGMCC 1.11031).

  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41176130, 41106161, 31170055, 31070061 and 31025001)
  • Hi-Tech Research and Development program of China (Award 2012AA092103 and 2011AA090703)
  • Special Fund of China for Marine-scientific Research in the Public Interest (Award 201005032-6)
  • Natural Science Foundation of Shandong Province (Award ZR2009DZ002 and JQ200910)
  • Foundation for Young Excellent Scientists in Shandong Province (Award BS2010SW015)
  • China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (Award DY125-15-T-05 and DY125-13-E-01)
© 2013 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.046656-0
2013-06-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/6/2248.html?itemId=/content/journal/ijsem/10.1099/ijs.0.046656-0&mimeType=html&fmt=ahah

References

  1. Abraham W.-R., Strömpl C., Meyer H., Lindholst S., Moore E. R. B., Christ R., Vancanneyt M., Tindall B. J., Bennasar A. et al. ( 1999 ). Phylogeny and polyphasic taxonomy of Caulobacter species. Proposal of Maricaulis gen. nov. with Maricaulis maris (Poindexter) comb. nov. as the type species, and emended description of the genera Brevundimonas and Caulobacter . . Int J Syst Bacteriol 49, 1053–1073. [View Article] [PubMed]
    [Google Scholar]
  2. Abraham W.-R., Strömpl C., Vancanneyt M., Bennasar A., Swings J., Lünsdorf H., Smit J., Moore E. R. ( 2004 ). Woodsholea maritima gen. nov., sp. nov., a marine bacterium with a low diversity of polar lipids. . Int J Syst Evol Microbiol 54, 1227–1234. [View Article] [PubMed]
    [Google Scholar]
  3. Alain K., Tindall B. J., Intertaglia L., Catala P., Lebaron P. ( 2008 ). Hellea balneolensis gen. nov., sp. nov., a prosthecate alphaproteobacterium from the Mediterranean Sea. . Int J Syst Evol Microbiol 58, 2511–2519. [View Article] [PubMed]
    [Google Scholar]
  4. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. ( 1997 ). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. . Nucleic Acids Res 25, 3389–3402. [View Article] [PubMed]
    [Google Scholar]
  5. Chen M. H., Sheu S. Y., Chen C. A., Wang J. T., Chen W. M. ( 2012 ). Oceanicaulis stylophorae sp. nov., isolated from the reef-building coral Stylophora pistillata . . Int J Syst Evol Microbiol 62, 2241–2246. [View Article] [PubMed]
    [Google Scholar]
  6. Collins M. D., Jones D. ( 1980 ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Bacteriol 48, 459–470. [CrossRef]
    [Google Scholar]
  7. Cowan S. T., Steel K. J. ( 1965 ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
     [Google Scholar]
  8. Fukui Y., Abe M., Kobayashi M., Saito H., Oikawa H., Yano Y., Satomi M. ( 2013 ). Algimonas porphyrae gen. nov., sp. nov., a member of the family Hyphomonadaceae, isolated from the red alga Porphyra yezoensis . . Int J Syst Evol Microbiol 63, 314–320. [View Article] [PubMed]
    [Google Scholar]
  9. Jung J. Y., Kim J. M., Jin H. M., Kim S. Y., Park W., Jeon C. O. ( 2011 ). Litorimonas taeanensis gen. nov., sp. nov., isolated from a sandy beach. . Int J Syst Evol Microbiol 61, 1534–1538. [View Article] [PubMed]
    [Google Scholar]
  10. Kang H. S., Lee S. D. ( 2009 ). Ponticaulis koreensis gen. nov., sp. nov., a new member of the family Hyphomonadaceae isolated from seawater. . Int J Syst Evol Microbiol 59, 2951–2955. [View Article] [PubMed]
    [Google Scholar]
  11. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  12. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111–120. [View Article] [PubMed]
    [Google Scholar]
  13. Komagata K., Suzuki K. ( 1987 ). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  14. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
     [Google Scholar]
  15. Lee K. B., Liu C. T., Anzai Y., Kim H., Aono T., Oyaizu H. ( 2005 ). The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov.. Int J Syst Evol Microbiol 55, 1907–1919. [View Article] [PubMed]
    [Google Scholar]
  16. Lee K., Lee H. K., Choi T.-H., Cho J.-C. ( 2007 ). Robiginitomaculum antarcticum gen. nov., sp. nov., a member of the family Hyphomonadaceae, from Antarctic seawater. . Int J Syst Evol Microbiol 57, 2595–2599. [View Article] [PubMed]
    [Google Scholar]
  17. Lee S. H., Shim J. K., Kim J. M., Choi H.-K., Jeon C. O. ( 2011 ). Henriciella litoralis sp. nov., isolated from a tidal flat, transfer of Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. and emended description of the genus Henriciella . . Int J Syst Evol Microbiol 61, 722–727. [View Article] [PubMed]
    [Google Scholar]
  18. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3, 208–218. [View Article]
    [Google Scholar]
  19. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109–118. [View Article] [PubMed]
    [Google Scholar]
  20. Moore R. L., Weiner R. M., Gebers R. ( 1984 ). Genus Hyphomonas Pongratz 1957 nom. rev. emend., Hyphomonas polymorpha Pongratz 1957 nom. rev. emend., and Hyphomonas neptunium (Leifson 1964) comb. nov. emend. (Hyphomicrobium neptunium). . Int J Syst Bacteriol 34, 71–73. [View Article]
    [Google Scholar]
  21. Murray R. G. E., Doetsch R. N., Robinow C. F. ( 1994 ). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  22. Quail Z.-X., Zeng D.-N., Xiao Y.-P., Roh S. W., Nam Y.-D., Chang H.-W., Yoon J.-H., Oh H.-M., Bae J.-W. ( 2009 ). Henriciella marina gen. nov., sp. nov., a novel member of the family Hyphomonadaceae isolated from the East Sea. . J Microbiol 47, 156–161. [View Article] [PubMed]
    [Google Scholar]
  23. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  24. Schlesner H., Bartels C., Sittig M., Dorsch M., Stackebrandt E. ( 1990 ). Taxonomic and phylogenetic studies on a new taxon of budding, hyphal Proteobacteria, Hirschia baltica gen. nov., sp. nov.. Int J Syst Bacteriol 40, 443–451. [View Article] [PubMed]
    [Google Scholar]
  25. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  26. Strömpl C., Hold G. L., Lünsdorf H., Graham J., Gallacher S., Abraham W.-R., Moore E. R. B., Timmis K. N. ( 2003 ). Oceanicaulis alexandrii gen. nov., sp. nov., a novel stalked bacterium isolated from a culture of the dinoflagellate Alexandrium tamarense (Lebour) Balech. . Int J Syst Evol Microbiol 53, 1901–1906. [View Article] [PubMed]
    [Google Scholar]
  27. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  28. Zhang Z., Schwartz S., Wagner L., Miller W. ( 2000 ). A greedy algorithm for aligning DNA sequences. . J Comput Biol 7, 203–214. [View Article] [PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.046656-0
Loading
Marinicauda pacifica gen. nov., sp. nov., a prosthecate alphaproteobacterium of the family Hyphomonadaceae isolated from deep seawater
Int J Syst Evol Microbiol 63, 2248 (2013); https://doi.org/10.1099/ijs.0.046656-0
/content/journal/ijsem/10.1099/ijs.0.046656-0
/content/journal/ijsem/10.1099/ijs.0.046656-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error