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Abstract

A marine prosthecate bacterium, designated strain P-1 km-3, was isolated from deep seawater from the Pacific. Cells of strain P-1 km-3 were Gram-stain-negative, aerobic, catalase- and oxidase-positive, dimorphic rods with a single polar prostheca or flagellum. The strain hydrolysed gelatin and grew at 6–40 °C (optimum, 30 °C) and with 0.5–12 % (w/v) NaCl (optimum, 2 %). Phylogenetic analysis of the 16S rRNA gene sequences revealed that strain P-1 km-3 belonged to the family in the class and represented a separate lineage, located between the genera and . Sequence similarities of strain P-1 km-3 with type strains of species of the genera and were 93.2–93.9 %. The predominant cellular fatty acids in strain P-1 km-3 were Cω7, C, 11-methyl Cω7, C and C cyclo ω8. The major respiratory quinone of strain P-1 km-3 was Q-10. The polar lipids of strain P-1 km-3 comprised glucuronopyranosyldiglyceride (GUDG), monoglycosyldiglyceride (MGDG), sulfo-quinovosyl diacylglycerol (SQDG), phosphatidylglycerol (PG), an unidentified phospholipid (PL) and an unidentified lipid (L). The genomic DNA G+C content of strain P-1 km-3 was 66.0 mol%. On the basis of the polyphasic data presented in this study, strain P-1 km-3 is proposed to represent a novel species in a new genus, gen. nov., sp. nov., within the family . The type strain of the type species is P-1 km-3 ( = KACC 16526 = CGMCC 1.11031).

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2013-06-01
2019-10-18
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