1887

Abstract

’ infects micronuclei of (, ), whereas ‘’ infects the macronucleus in other clones of the same host species. Because these micro-organisms have not been cultivated, their description has been based only on some morphological properties and host and nuclear specificities. One16S rRNA gene sequence of ‘ ’ is present in databases. The systematic position of the representative strain of ‘ ’, strain MC-3, was determined in this study. Moreover, for the first time, two strains of ‘ ’, KBN10-1 and AC61-10, were investigated. Phylogenetic analysis indicated that all three strains belonged to the genus family , order within the .

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2013-05-01
2019-12-08
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References

  1. Amann R. , Springer N. , Ludwig W. , Görtz H. D. , Schleifer K. H. . ( 1991; ). Identification in situ and phylogeny of uncultured bacterial endosymbionts. . Nature 351:, 161–164. [CrossRef] [PubMed]
    [Google Scholar]
  2. Borchsenius O. N. , Skoblo I. I. , Ossipov D. V. . ( 1983; ). Holospora curviuscula – a new species of macronuclear symbiotic bacteria of Paramecium bursaria . . Tsitologiya 25:, 91–97.
    [Google Scholar]
  3. Boscaro V. , Fokin S. I. , Schrallhammer M. , Schweikert M. , Petroni G. . ( 2012; ). Revised systematics of Holospora-like bacteria and characterization of ‘Candidatus Gortzia infectiva’, a novel macronuclear symbiont of Paramecium jenningsi . . Microb Ecol 65:, 255–267.[CrossRef]
    [Google Scholar]
  4. Eschbach E. , Pfannkuchen M. , Schweikert M. , Drutschmann D. , Brümmer F. , Fokin S. , Ludwig W. , Görtz H. D. . ( 2009; ). Candidatus Paraholospora nucleivisitans’, an intracellular bacterium in Paramecium sexaurelia shuttles between the cytoplasm and the nucleus of its host. . Syst Appl Microbiol 32:, 490–500. [CrossRef] [PubMed]
    [Google Scholar]
  5. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  6. Görtz H. D. , Schmidt H. J. . ( 2005; ). Holosporaceae . . In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 146–160. Edited by Garrity G. M. . . New York:: Springer;.
    [Google Scholar]
  7. Grimont F. , Grimont P. A. D. . ( 1995; ). Determination of rRNA gene restriction patterns. . In Methods in Molecular Biology, pp. 181–200. Edited by Howard J. , Whitcombe D. M. . . New York:: Humana Press;.
    [Google Scholar]
  8. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kishino H. , Hasegawa M. . ( 1989; ). Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in hominoidea . . J Mol Evol 29:, 170–179. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kudo R. . ( 1966; ). Protozoology. Springfield, IL:: Charles C. Thomas;.
    [Google Scholar]
  11. Lang B. F. , Brinkman H. , Koski L. B. , Fujishima M. , Görtz H.-D. , Burger G. . ( 2005; ). On the origin of mitochondria and Rickettsia-related eukaryotic endosymbionts. . Jpn J Protozool 38:, 171–183.
    [Google Scholar]
  12. Ossipov D. V. , Skoblo I. I. , Borchsenius O. N. , Rautian M. S. , Podlipaev S. A. . ( 1980; ). Holospora acuminata – a new species of symbiotic bacterium from the micronucleus of the ciliate Paramecium bursaria Focke. . Tsitologiya 22:, 922–929.
    [Google Scholar]
  13. Preer J. R. Jr . ( 1977; ). The killer system in Paramecium-kappa and its viruses. . Microbiology 1977:, 576–578.
    [Google Scholar]
  14. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  15. Tamura A. , Urakami H. , Tsuruhara T. . ( 1982; ). Purification of Rickettsia tsutsugamushi by Percoll density gradient centrifugation. . Microbiol Immunol 26:, 321–328.[PubMed] [CrossRef]
    [Google Scholar]
  16. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  17. Vakkerov-Kouzova N. D. , Rautian M. S. . ( 2011; ). Obtaining and characterization of ‘Holospora curviuscula’ and Holospora obtusa, bacterial symbionts of the macronuclei of Paramecium bursaria and Paramecium caudatum . . Mikrobiologiia 80:, 714–718.
    [Google Scholar]
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