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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_7

sp. nov., an amylolytic bacterium of the family isolated from coastal surface seawater Free

Abstract

A novel Gram-negative, rod-shaped, non-motile bacterium, designated strain N1, was isolated from a marine water sample collected from the sea shore, Bay of Bengal, Visakhapatnam, India. The strain was positive for starch hydrolysis, nitrate reduction and ornithine decarboxylase activities and negative for citrate utilization, urease, oxidase, catalase and DNase activities. The predominant fatty acids were C 3-OH, iso-C, iso-C 3-OH, iso-C 3-OH, anteiso-C, C, C 3-OH, and Cω7 and/or iso-C 2-OH (summed feature 3). Strain N1 contained menaquinone 6 (MK-6) as the sole respiratory quinone. The only polyamine was homospermidine and the major polar lipids were phosphatidylethanolamine (PE), three unidentified aminolipids (AL1–AL3) and two unidentified lipids (L1, L2). The DNA G+C content of the strain was 36.3 mol%. 16S rRNA gene sequence analysis indicated that strain N1 was a member of the genus and closely related to with pairwise sequence similarity of 96.5 %. Phylogenetic analysis showed that strain N1 clustered with and with a distance of 4.8 and 6.0 % (95.2 and 94.0 % similarity), respectively. Based on the phenotypic characteristics and on phylogenetic inference, strain N1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is N1 ( = MTCC 11155 = JCM 17678).

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Funding
This study was supported by the:
  • Council of Scientific and Industrial Research (CSIR)
  • Department of Biotechnology, Government of India
© 2013 IUMS
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2013-07-01
2024-04-20
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Flavobacterium nitratireducens sp. nov., an amylolytic bacterium of the family Flavobacteriaceae isolated from coastal surface seawater
Int J Syst Evol Microbiol 63, 2490 (2013); https://doi.org/10.1099/ijs.0.046524-0
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