1887

Abstract

A novel Gram-negative, rod-shaped, non-motile bacterium, designated strain N1, was isolated from a marine water sample collected from the sea shore, Bay of Bengal, Visakhapatnam, India. The strain was positive for starch hydrolysis, nitrate reduction and ornithine decarboxylase activities and negative for citrate utilization, urease, oxidase, catalase and DNase activities. The predominant fatty acids were C 3-OH, iso-C, iso-C 3-OH, iso-C 3-OH, anteiso-C, C, C 3-OH, and Cω7 and/or iso-C 2-OH (summed feature 3). Strain N1 contained menaquinone 6 (MK-6) as the sole respiratory quinone. The only polyamine was homospermidine and the major polar lipids were phosphatidylethanolamine (PE), three unidentified aminolipids (AL1–AL3) and two unidentified lipids (L1, L2). The DNA G+C content of the strain was 36.3 mol%. 16S rRNA gene sequence analysis indicated that strain N1 was a member of the genus and closely related to with pairwise sequence similarity of 96.5 %. Phylogenetic analysis showed that strain N1 clustered with and with a distance of 4.8 and 6.0 % (95.2 and 94.0 % similarity), respectively. Based on the phenotypic characteristics and on phylogenetic inference, strain N1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is N1 ( = MTCC 11155 = JCM 17678).

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2013-07-01
2019-10-23
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