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Abstract

To investigate the biodiversity of bacteria in the spring water of the Chengcing Lake Park in Taiwan, a Gram-stain-negative, rod-shaped, non-motile, non-spore-forming and aerobic bacterial strain, designated strain Chen16-4, was isolated and characterized in a taxonomic study using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the closest relatives of strain Chen16-4 were YT, GIFU 9882 and WP01, with sequence similarities of 97.6, 97.1 and 97.0 %, respectively. A phylogenetic tree obtained with 16S rRNA gene sequences indicated that strain Chen16-4 and these three closest relatives formed an independent phylogenetic clade within the genus . The polar lipid pattern, the presence of spermidine and ubiquinone Q-10, the predominance of Cω7 in the cellular fatty acid profile and the DNA G+C content also supported affiliation of the isolate to the genus . The DNA–DNA relatedness of strain Chen16-4 with respect to recognized species of the genus was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Chen16-4 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is Chen16-4 ( = BCRC 80308 = LMG 26342 = KCTC 23559).

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2013-05-01
2024-03-29
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