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Volume 63, Issue Pt_7

sp. nov., a psychrotolerant, phototrophic alphaproteobacterium isolated from a freshwater stream Free

Abstract

Two strains (JA643 and JA755) of Gram-stain-negative, facultatively anaerobic phototrophic, bacteria capable of growth at low temperatures (10–15 °C) were isolated from freshwater streams from different geographical regions of India. Both strains contain bacteriochlorophyll and carotenoids of the spirilloxanthin series. Phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid (PL), unidentified amino lipids (AL1–AL6, AL9) and an unidentified lipid (L1) were the polar lipids present in both strains. The major cellular fatty acid was Cω7 (76–79 % of the total). Bacteriohopane derivatives (BHD1,2), unidentified hopanoids (UH1–5), diplopterol (DPL) and diploptene (DPE) were the major hopanoids of both strains. The DNA G+C content was 64.2–64.5 mol%. 16S rRNA gene sequence-based phylogenetic analysis showed that both strains are closely related to the genus and clustered with DSM 162 (99 % sequence similarity). However, both strains exhibited only 46.1 % DNA–DNA hybridization with DSM 162. Strains JA643 and JA755 shared >99 % 16S rRNA gene sequence similarity and were >85 % related on the basis of DNA–DNA hybridization; they are therefore considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is JA643 ( = KCTC 15219 = NBRC 109057).

  • Published Online:
Funding
This study was supported by the:
  • UGC, Government of India
  • JNTU Hyderabad
  • CSIR
© 2013 IUMS
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2013-07-01
2023-03-27
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Rhodomicrobium udaipurense sp. nov., a psychrotolerant, phototrophic alphaproteobacterium isolated from a freshwater stream
Int J Syst Evol Microbiol 63, 2684 (2013); https://doi.org/10.1099/ijs.0.046409-0
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