1887

Abstract

Strain JC141 was isolated from an alkaline soil (pH 8.8) at Mau, Uttar Pradesh, India. Colonies were blue with a metallic sheen; cells stained Gram-negative, and were oxidase- and catalase-positive, but chitinase-negative. Major fatty acids were Cω7/Cω6, C and C with minor amounts of C, C 3-OH, C, C and Cω7. Strain JC141 contained phosphatidylethanolamine and phosphatidylglycerol as major polar lipids, with minor amounts of diphosphatidylglycerol, unidentified aminolipids (AL1–3) and unidentified lipids (L1–4). A bacterial hopane derivative (BHD1) was the major hopanoid. Genomic DNA G+C content was 62.5 mol%. 16S rRNA gene sequence comparisons indicated that strain JC141 represents a member of the genus within the family of the class . Strain JC141 had a 16S rRNA gene sequence similarity of 98 % with ATCC 19706 and <96.5 % with other members of the genus . However, strain JC141 showed 45.8 % relatedness (based on DNA–DNA hybridization) with LMG 6867 ( = ATCC 19706). Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain JC141 as a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is JC141 ( = KCTC 32041 = LMG 27066). An emended description of the genus is also proposed.

Funding
This study was supported by the:
  • , UGC-BSR-RFSMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.046300-0
2013-06-01
2020-08-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/6/2338.html?itemId=/content/journal/ijsem/10.1099/ijs.0.046300-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. ( 1978 ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . . Proc Natl Acad Sci U S A 75, 48014805. [CrossRef] [PubMed]
    [Google Scholar]
  3. Cappuccino J. G., Sherman N. ( 1998 ). Microbiology – A Laboratory Manual, , 5th edn. San Francisco:: Benjamin/Cummings Science Publishing;.
    [Google Scholar]
  4. Chèneby D., Philippot L., Hartmann A., Hénault C., Germon J. C. ( 2000 ). 16S rDNA analysis for characterization of denitrifying bacteria isolated from three agricultural soils. . FEMS Microbiol Ecol 34, 121128. [CrossRef] [PubMed]
    [Google Scholar]
  5. Chou Y. J., Chou J. H., Lin M. C., Arun A. B., Young C. C., Chen W. M. ( 2008 ). Vogesella perlucida sp. nov., a non-pigmented bacterium isolated from spring water. . Int J Syst Evol Microbiol 58, 26772681. [CrossRef] [PubMed]
    [Google Scholar]
  6. Chou J. H., Chou Y. J., Arun A. B., Young C. C., Chen C. A., Wang J. T., Chen W. M. ( 2009 ). Vogesella lacus sp. nov., isolated from a soft-shell turtle culture pond. . Int J Syst Evol Microbiol 59, 26292632. [CrossRef] [PubMed]
    [Google Scholar]
  7. Grimes D. J., Woese C. R., MacDonell M. T., Colwell R. R. ( 1997 ). Systematic study of the genus Vogesella gen. nov. and its type species, Vogesella indigofera comb. nov.. Int J Syst Bacteriol 47, 1927. [CrossRef] [PubMed]
    [Google Scholar]
  8. Hiraishi A., Hoshino Y. ( 1984 ). Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. . J Gen Appl Microbiol 30, 435448. [CrossRef]
    [Google Scholar]
  9. Hiraishi A., Hoshino Y., Kitamura H. ( 1984 ). Isoprenoid 236 quinone composition in the classification of Rhodospirillaceae . . J Gen Appl Microbiol 30, 197210. [CrossRef]
    [Google Scholar]
  10. Imhoff J. F. ( 1984 ). Quinones of phototrophic purple bacteria. . FEMS Microbiol Lett 25, 8589. [CrossRef]
    [Google Scholar]
  11. Imhoff J. F., Pfennig N. ( 2001 ). Thioflavicoccus mobilis gen. nov., sp. nov., a novel purple sulfur bacterium with bacteriochlorophyll b . . Int J Syst Evol Microbiol 51, 105110.[PubMed]
    [Google Scholar]
  12. Imhoff J. F., Süling J., Petri R. ( 1998 ). Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium . . Int J Syst Bacteriol 48, 11291143. [CrossRef] [PubMed]
    [Google Scholar]
  13. Jørgensen N. O. G., Brandt K. K., Nybroe O., Hansen M. ( 2010 ). Vogesella mureinivorans sp. nov., a peptidoglycan-degrading bacterium from lake water. . Int J Syst Evol Microbiol 60, 24672472. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kates M. ( 1986 ). Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids. Amsterdam:: Elsevier;.
    [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  16. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [CrossRef] [PubMed]
    [Google Scholar]
  17. Krieg N. R. ( 2005 ). Genus Vogesella . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, pp. 858859. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. . New York:: Springer;. [CrossRef]
    [Google Scholar]
  18. Kuhn R., Starr M. P., Kuhn D. A., Bauer H., Knackmuss H. J. ( 1965 ). Indigoidine and other bacterial pigments related to 3–3′-bipyridyl. . Arch Microbiol 51, 7184.
    [Google Scholar]
  19. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. ( 1985 ). Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. . Proc Natl Acad Sci U S A 82, 69556959. [CrossRef] [PubMed]
    [Google Scholar]
  20. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [CrossRef]
    [Google Scholar]
  21. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  22. Raj P. S., Ramaprasad E. V. V., Vaseef S., Sasikala Ch., Ramana Ch. V. ( 2012 ). Rhodobacter viridis sp. nov., a phototrophic bacterium isolated from mud of a stream. . Int J Syst Evol Microbiol 63, 181186. [PubMed] [CrossRef]
    [Google Scholar]
  23. Rohmer M., Bouvier-Nave P., Ourisson G. ( 1984 ). Distribution of hopanoid triterpenes in prokaryotes. . J Gen Microbiol 130, 11371150.
    [Google Scholar]
  24. Sasser M. ( 1990 ). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  25. Seldin L., Dubnau D. ( 1985 ). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. . Int J Syst Bacteriol 35, 151154. [CrossRef]
    [Google Scholar]
  26. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [CrossRef] [PubMed]
    [Google Scholar]
  27. Trüper H. G., Pfennig N. ( 1981 ). Isolation of members of the families Chromatiaceae and Chlorobiaceae . . In The Prokaryotes: a Handbook on Habitats, Isolation, and Identification of Bacteria, pp. 279289. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. . Berlin:: Springer;.
    [Google Scholar]
  28. Tyo K. E., Zhou H., Stephanopoulos G. N. ( 2006 ). High-throughput screen for poly-3-hydroxybutyrate in Escherichia coli and Synechocystis sp. strain PCC6803. . Appl Environ Microbiol 72, 34123417. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.046300-0
Loading
/content/journal/ijsem/10.1099/ijs.0.046300-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error