%0 Journal Article %A Gaboyer, Frédéric %A Tindall, Brian J. %A Ciobanu, Maria-Cristina %A Duthoit, Frédérique %A Le Romancer, Marc %A Alain, Karine %T Phaeobacterleonis sp. nov., an alphaproteobacterium from Mediterranean Sea sediments %D 2013 %J International Journal of Systematic and Evolutionary Microbiology, %V 63 %N Pt_9 %P 3301-3306 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.046128-0 %I Microbiology Society, %X A novel Gram-stain-negative, strictly aerobic, heterotrophic bacterium, designated 306T, was isolated from near-surface (109 cm below the sea floor) sediments of the Gulf of Lions, in the Mediterranean Sea. Strain 306T grew at temperatures between 4 and 32 °C (optimum 17–22 °C), from pH 6.5 to 9.0 (optimum 8.0–9.0) and between 0.5 and 6.0 % (w/v) NaCl (optimum 2.0 %). Its DNA G+C content was 58.8 mol%. On the basis of 16S rRNA gene sequence similarity, the novel isolate belongs to the class Alphaproteobacteria and is related to the genus Phaeobacter . It shares 98.7 % 16S rRNA sequence identity with Phaeobacter arcticus , its closest phylogenetic relative. It contained Q-10 as the only respiratory quinone, C18 : 1ω7c and C16 : 0 as major fatty acids (>5 %) and phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, two unidentified lipids and an aminolipid as polar lipids. The chemotaxonomic data are consistent with the affiliation of strain 306T to the genus Phaeobacter . Results of physiological experiments, biochemical tests and DNA–DNA hybridizations (with P. arcticus ) indicate that strain 306T is genetically and phenotypically distinct from the five species of the genus Phaeobacter with validly published names. Strain 306T therefore represents a novel species, for which the name Phaeobacter leonis sp. nov. is proposed. The type strain is 306T ( = DSM 25627T = CIP 110369T = UBOCC 3187T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.046128-0