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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_9

sp. nov., an alphaproteobacterium from Mediterranean Sea sediments Free

Abstract

A novel Gram-stain-negative, strictly aerobic, heterotrophic bacterium, designated 306, was isolated from near-surface (109 cm below the sea floor) sediments of the Gulf of Lions, in the Mediterranean Sea. Strain 306 grew at temperatures between 4 and 32 °C (optimum 17–22 °C), from pH 6.5 to 9.0 (optimum 8.0–9.0) and between 0.5 and 6.0 % (w/v) NaCl (optimum 2.0 %). Its DNA G+C content was 58.8 mol%. On the basis of 16S rRNA gene sequence similarity, the novel isolate belongs to the class and is related to the genus . It shares 98.7 % 16S rRNA sequence identity with its closest phylogenetic relative. It contained Q-10 as the only respiratory quinone, Cω7 and C as major fatty acids (>5 %) and phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, two unidentified lipids and an aminolipid as polar lipids. The chemotaxonomic data are consistent with the affiliation of strain 306 to the genus . Results of physiological experiments, biochemical tests and DNA–DNA hybridizations (with ) indicate that strain 306 is genetically and phenotypically distinct from the five species of the genus with validly published names. Strain 306 therefore represents a novel species, for which the name sp. nov. is proposed. The type strain is 306 ( = DSM 25627 = CIP 110369 = UBOCC 3187).

  • Published Online:
Funding
This study was supported by the:
  • Joint Research Unit UMR 6197 [Centre National de la Recherche Scientifique (CNRS)–Institut Français de Recherche pour l'Exploitation de la Mer (Ifremer)–Université de Bretagne Occidentale (UBO)]
  • Institut National des Sciences de l'Univers (INSU) (CNRS)
  • UBO
  • Région Bretagne
  • French Ministry of Higher Education and Research
© 2013 IUMS
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2013-09-01
2024-09-15
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References

  1. Alain K., Querellou J., Lesongeur F., Pignet P., Crassous P., Raguénès G., Cueff V., Cambon-Bonavita M.-A. ( 2002 ). Caminibacter hydrogeniphilus gen. nov., sp. nov., a novel thermophilic, hydrogen-oxidizing bacterium isolated from an East Pacific Rise hydrothermal vent. . Int J Syst Evol Microbiol 52, 1317–1323. [View Article] [PubMed]
    [Google Scholar]
  2. Bligh E. G., Dyer W. J. ( 1959 ). A rapid method of total lipid extraction and purification. . Can J Biochem Physiol 37, 911–917. [View Article] [PubMed]
    [Google Scholar]
  3. Ciobanu M.-C., Rabineau M., Droz L., Révillon S., Ghiglione J.-F., Dennielou B., Jorry S.-J., Kallmeyer J., Etoubleau J. & other authors ( 2012 ). Sedimentological imprint on subseafloor microbial communities in Western Mediterranean Sea Quaternary sediments. . Biogeosciences 9, 3491–3512. [View Article]
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133–142. [View Article] [PubMed]
    [Google Scholar]
  5. DeLong E. F. ( 1992 ). Archaea in coastal marine environments. . Proc Natl Acad Sci U S A 89, 5685–5689. [View Article] [PubMed]
    [Google Scholar]
  6. Gerhardt P., Murray R., Wood W., Krieg N. ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology Press;.
     [Google Scholar]
  7. Huss V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184–192. [View Article] [PubMed]
    [Google Scholar]
  8. Kuykendall L., Roy M., O’neill J., Devine T. ( 1988 ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38, 358–361. [View Article]
    [Google Scholar]
  9. Martens T., Heidorn T., Pukall R., Simon M., Tindall B. J., Brinkhoff T. ( 2006 ). Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera . . Int J Syst Evol Microbiol 56, 1293–1304. [View Article] [PubMed]
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39, 159–167. [View Article]
    [Google Scholar]
  11. Ruiz-Ponte C., Cilia V., Lambert C., Nicolas J. L. ( 1998 ). Roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop Pecten maximus . . Int J Syst Bacteriol 48, 537–542. [View Article] [PubMed]
    [Google Scholar]
  12. Stackebrandt E., Ebers J. ( 2006 ). Taxonomic parameters revisited: tarnished gold standards. . Microbiol Today 33, 152–155.
     [Google Scholar]
  13. Tindall B. ( 1990a ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128–130. [View Article]
    [Google Scholar]
  14. Tindall B. ( 1990b ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199–202. [View Article]
    [Google Scholar]
  15. Tindall B., Sikorski J., Smibert R. A., Krieg N. R. ( 2007 ). Phenotypic Characterization and the Principles of Comparative Systematics. . In Methods for General and Molecular Microbiology . , 3rd edition.. pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. . Washington DC:: American Society for Microbiology;.
     [Google Scholar]
  16. Vandecandelaere I., Segaert E., Mollica A., Faimali M., Vandamme P. ( 2009 ). Phaeobacter caeruleus sp. nov., a blue-coloured, colony-forming bacterium isolated from a marine electroactive biofilm. . Int J Syst Evol Microbiol 59, 1209–1214. [View Article] [PubMed]
    [Google Scholar]
  17. Yoon J.-H., Kang S.-J., Lee S.-Y., Oh T.-K. ( 2007 ). Phaeobacter daeponensis sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 57, 856–861. [View Article] [PubMed]
    [Google Scholar]
  18. Zhang D.-C., Li H.-R., Xin Y. H., Liu H.-C., Chi Z.-M., Zhou P.-J., Yu Y. ( 2008 ). Phaeobacter arcticus sp. nov., a psychrophilic bacterium isolated from the Arctic. . Int J Syst Evol Microbiol 58, 1384–1387. [View Article] [PubMed]
    [Google Scholar]
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Phaeobacterleonis sp. nov., an alphaproteobacterium from Mediterranean Sea sediments
Int J Syst Evol Microbiol 63, 3301 (2013); https://doi.org/10.1099/ijs.0.046128-0
/content/journal/ijsem/10.1099/ijs.0.046128-0
/content/journal/ijsem/10.1099/ijs.0.046128-0
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