1887

Abstract

Three Gram-negative strains, NF 1078, NF 1598 and NF 1715, were isolated from clinical (two) and environmental (one) samples, respectively. Sequence analysis of the 16S rRNA genes revealed similarity of 100 % among the three strains and next highest similarity to the type strain of (98.16 %). The three strains were able to acidify lactose and rhamnose on low peptone phenol red agar and to alkalinize citrate on Simmons’ agar and were negative for nitrate reduction. The DNA G+C content of strain NF 1078 was 67.1 mol%. The level of DNA–DNA relatedness between this strain and the type strains of (ATCC 19860, LMG 2117) was 29 %. Based on these phylogenetic, phenotypic and genotypic analyses, the three strains could be distinguished clearly from all other recognized species and should be classified as representatives of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NF 1078 ( = LMG 26971 = CCUG 62584).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.046102-0
2013-06-01
2019-10-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/6/2203.html?itemId=/content/journal/ijsem/10.1099/ijs.0.046102-0&mimeType=html&fmt=ahah

References

  1. Castagnola E. , Conte M. , Venzano P. , Garaventa A. , Viscoli C. , Barretta M. A. , Pescetto L. , Tasso L. , Nantron M. et al. ( 1997; ). Broviac catheter-related bacteraemias due to unusual pathogens in children with cancer: case reports with literature review. . J Infect 34:, 215–218. [CrossRef] [PubMed]
    [Google Scholar]
  2. Cooper G. R. , Staples E. D. , Iczkowski K. A. , Clancy C. J. . ( 2005; ). Comamonas (Pseudomonas) testosteroni endocarditis. . Cardiovasc Pathol 14:, 145–149. [CrossRef] [PubMed]
    [Google Scholar]
  3. Ender P. T. , Dooley D. P. , Moore R. H. . ( 1996; ). Vascular catheter-related Comamonas acidovorans bacteremia managed with preservation of the catheter. . Pediatr Infect Dis J 15:, 918–920. [CrossRef] [PubMed]
    [Google Scholar]
  4. Franzetti F. , Cernuschi M. , Esposito R. , Moroni M. . ( 1992; ). Pseudomonas infections in patients with AIDS and AIDS-related complex. . J Intern Med 231:, 437–443. [CrossRef] [PubMed]
    [Google Scholar]
  5. Gul M. , Ciragil P. , Bulbuloglu E. , Aral M. , Alkis S. , Ezberci F. . ( 2007; ). Comamonas testosteroni bacteremia in a patient with perforated acute appendicitis. . Acta Microbiol Immunol Hung 54:, 317–321. [CrossRef] [PubMed]
    [Google Scholar]
  6. Horowitz H. , Gilroy S. , Feinstein S. , Gilardi G. . ( 1990; ). Endocarditis associated with Comamonas acidovorans . . J Clin Microbiol 28:, 143–145.[PubMed]
    [Google Scholar]
  7. Laffineur K. , Janssens M. , Charlier J. , Avesani V. , Wauters G. , Delmée M. . ( 2002; ). Biochemical and susceptibility tests useful for identification of nonfermenting gram-negative rods. . J Clin Microbiol 40:, 1085–1087. [CrossRef] [PubMed]
    [Google Scholar]
  8. Lair M. I. , Bentolila S. , Grenet D. , Cahen P. , Honderlick P. . ( 1996; ). Oerskovia turbata and Comamonas acidovorans bacteremia in a patient with AIDS. . Eur J Clin Microbiol Infect Dis 15:, 424–426. [CrossRef] [PubMed]
    [Google Scholar]
  9. Le Moal G. , Paccalin M. , Breux J. P. , Roblot F. , Roblot P. , Becq-Giraudon B. . ( 2001; ). Central venous catheter-related infection due to Comamonas testosteroni in a woman with breast cancer. . Scand J Infect Dis 33:, 627–628. [CrossRef] [PubMed]
    [Google Scholar]
  10. Lee S. M. , Kim M. K. , Lee J. L. , Wee W. R. , Lee J. H. . ( 2008; ). Experience of Comamonas acidovorans keratitis with delayed onset and treatment response in immunocompromised cornea. . Korean J Ophthalmol 22:, 49–52. [CrossRef] [PubMed]
    [Google Scholar]
  11. Lema I. , Gómez-Torreiro M. , Rodríguez-Ares M. T. . ( 2001; ). Comamonas acidovorans keratitis in a hydrogel contact lens wearer. . CLAO J 27:, 55–56.[PubMed]
    [Google Scholar]
  12. Malkan A. D. , Strollo W. , Scholand S. J. , Dudrick S. J. . ( 2009; ). Implanted-port-catheter-related sepsis caused by Acidovorax avenae and methicillin-sensitive Staphylococcus aureus . . J Clin Microbiol 47:, 3358–3361. [CrossRef] [PubMed]
    [Google Scholar]
  13. Miño de Kaspar H. , Grasbon T. , Kampik A. . ( 2000; ). Automated surgical equipment requires routine disinfection of vacuum control manifold to prevent postoperative endophthalmitis. . Ophthalmology 107:, 685–690. [CrossRef] [PubMed]
    [Google Scholar]
  14. Nseir W. , Khateeb J. , Awawdeh M. , Ghali M. . ( 2011; ). Catheter-related bacteremia caused by Comamonas testosteroni in a hemodialysis patient. . Hemodial Int 15:, 293–296. [CrossRef] [PubMed]
    [Google Scholar]
  15. Reddy A. K. , Murthy S. I. , Jalali S. , Gopinathan U. . ( 2009; ). Post-operative endophthalmitis due to an unusual pathogen, Comamonas testosteroni . . J Med Microbiol 58:, 374–375. [CrossRef] [PubMed]
    [Google Scholar]
  16. Shetty A. , Barnes R. A. , Healy B. , Groves P. . ( 2005; ). A case of sepsis caused by Acidovorax . . J Infect 51:, e171–e172. [CrossRef] [PubMed]
    [Google Scholar]
  17. Siebor E. , Llanes C. , Lafon I. , Ogier-Desserrey A. , Duez J. M. , Pechinot A. , Caillot D. , Grandjean M. , Sixt N. , Neuwirth C. . ( 2007; ). Presumed pseudobacteremia outbreak resulting from contamination of proportional disinfectant dispenser. . Eur J Clin Microbiol Infect Dis 26:, 195–198. [CrossRef] [PubMed]
    [Google Scholar]
  18. Smith M. D. , Gradon J. D. . ( 2003; ). Bacteremia due to Comamonas species possibly associated with exposure to tropical fish. . South Med J 96:, 815–817. [CrossRef] [PubMed]
    [Google Scholar]
  19. Stonecipher K. G. , Jensen H. G. , Kastl P. R. , Faulkner A. , Rowsey J. J. . ( 1991; ). Ocular infections associated with Comamonas acidovorans. . Am J Ophthalmol 112:, 46–49.[PubMed] [CrossRef]
    [Google Scholar]
  20. Tsui T. L. , Tsao S. M. , Liu K. S. , Chen T. Y. , Wang Y. L. , Teng Y. H. , Lee Y. T. . ( 2011; ). Comamonas testosteroni infection in Taiwan: reported two cases and literature review. . J Microbiol Immunol Infect 44:, 67–71. [CrossRef] [PubMed]
    [Google Scholar]
  21. Vaneechoutte M. , Rossau R. , De Vos P. , Gillis M. , Janssens D. , Paepe N. , De Rouck A. , Fiers T. , Claeys G. , Kersters K. . ( 1992; ). Rapid identification of bacteria of the Comamonadaceae with amplified ribosomal DNA-restriction analysis (ARDRA). . FEMS Microbiol Lett 93:, 227–233. [CrossRef] [PubMed]
    [Google Scholar]
  22. Wauters G. , Vaneechoutte M. . ( 2011; ). Approaches to the identification of aerobic Gram-negative bacteria. . In Manual of Clinical Microbiology, , 10th edn., pp. 539–558. Edited by Versalovic J. , Carroll K. A. , Funke G. , Jorgensen J. H. , Landry M. L. , Warnock D. W. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  23. Wauters G. , Avesani V. , Laffineur K. , Charlier J. , Janssens M. , Van Bosterhaut B. , Delmée M. . ( 2003; ). Brevibacterium lutescens sp. nov., from human and environmental samples. . Int J Syst Evol Microbiol 53:, 1321–1325. [CrossRef] [PubMed]
    [Google Scholar]
  24. Willems A. , Falsen E. , Pot B. , Jantzen E. , Hoste B. , Vandamme P. , Gillis M. , Kersters K. , De Ley J. . ( 1990; ). Acidovorax, a new genus for Pseudomonas facilis, Pseudomonas delafieldii, E. Falsen (EF) group 13, EF group 16 and several clinical isolates with the species Acidovorax comb. nov., Acidovorax delafieldii comb. nov., and Acidovorax temperans sp. nov. . Int J Syst Bacteriol 40:, 384–398. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.046102-0
Loading
/content/journal/ijsem/10.1099/ijs.0.046102-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error