A taxonomic study was carried out on strain DLFJ1-1T, which was isolated from an oil-degrading bacterial consortium, enriched by the surface seawater from around the Dalian Peninsula. The isolate was Gram-reaction-negative, oxidase- and catalase-positive, short-rod-shaped and non-motile. Growth was observed at salinities from 0.5 to 15 % and at temperatures from 4 to 37 °C; the strain was unable to degrade gelatin or to reduce nitrate. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DLFJ1-1T belonged to the genus Thioclava, with the highest sequence similarity (96.4 %) to Thioclava pacifica TL 2T, while the similarities to other species examined were all below 95.0 %. The principal fatty acids were C19 : 0ω8c cyclo and summed feature 8 (C18 : 1ω7c/ω6c). The major quinone of strain DLFJ1-1T was Q10. The major polar lipids of strain DLFJ1-1T were phosphatidylethanolamine and phosphatidylglycerol. The G+C content of the chromosomal DNA was 62.5 mol%. The combined genotypic and phenotypic data show that strain DLFJ1-1T represents a novel species of the genus Thioclava, for which the name Thioclava dalianensis sp. nov. is proposed, with the type strain DLFJ1-1T ( = CGMCC 1.12325T = LMG 27290T = MCCC 1A03957T). An emended description of the genus Thioclava is also proposed.
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Funding
This study was supported by the:
State Oceanic Administration (SOA)
(Award 201005032)
National Infrastructure of Natural Resources for Science and Technology Program of China
(Award NIMR-2011-9)
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