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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 63, Issue Pt_8

gen. nov., sp. nov., an actinobacterium isolated from reservoir water Free

Abstract

A novel Gram-reaction-positive bacterium, strain CH22-21, was isolated from a water sample taken from Daechung Reservoir, Republic of Korea, during the late-blooming period of cyanobacteria. Cells of strain CH22-21 were non-motile, ciliated short rods that formed creamy-white colonies on half-strength modified R2A agar. Chemotaxonomic results showed menaquinone MK-9(H) as the predominant respiratory menaquinone, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside as major polar lipids, 16 : 1ω5, 16 : 0, 17 : 1ω8 and 18 : 1ω9 as major fatty acids, and a DNA G+C content of 71.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the strain formed a separate lineage within the order , showing similarity values of <92.3 % with its closest phylogenetic neighbours. The combined genotypic and phenotypic data showed that strain CH22-21 could be distinguished from all genera within the order and represented a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is CH22-21 ( = KCTC 19849 = JCM 17787).

  • Published Online:
Funding
This study was supported by the:
  • Korean Ministry of Education, Science & Technology
  • Korean Ministry of Environment
© 2013 IUMS
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2013-08-01
2025-03-24
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References

  1. Clark D. A., Norris P. R. ( 1996 ). Acidimicrobium ferrooxidans gen. nov., sp. nov.: mixed-culture ferrous iron oxidation with Sulfobacillus species. . Microbiology 142, 785–790. [View Article]
    [Google Scholar]
  2. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [View Article] [PubMed]
    [Google Scholar]
  3. Felsenstein J. ( 1985 ). Confidence limit on phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  4. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406–416. [View Article]
    [Google Scholar]
  5. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucl Acids Symp Ser 41, 95–98.
     [Google Scholar]
  6. Itoh T., Yamanoi K., Kudo T., Ohkuma M., Takashina T. ( 2011 ). Aciditerrimonas ferrireducens gen. nov., sp. nov., an iron-reducing thermoacidophilic actinobacterium isolated from a solfataric field. . Int J Syst Evol Microbiol 61, 1281–1285. [View Article] [PubMed]
    [Google Scholar]
  7. Johnson D. B., Bacelar-Nicolau P., Okibe N., Thomas A., Hallberg K. B. ( 2009 ). Ferrimicrobium acidiphilum gen. nov., sp. nov. and Ferrithrix thermotolerans gen. nov., sp. nov.: heterotrophic, iron-oxidizing, extremely acidophilic actinobacteria. . Int J Syst Evol Microbiol 59, 1082–1089. [View Article] [PubMed]
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  9. Komagata K., Suzuki K.-I. ( 1987 ). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  10. Kumar S., Nei M., Dudley J., Tamura K. ( 2008 ). MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. . Brief Bioinform 9, 299–306. [View Article] [PubMed]
    [Google Scholar]
  11. Kurahashi M., Fukunaga Y., Sakiyama Y., Harayama S., Yokota A. ( 2009 ). Iamia majanohamensis gen. nov., sp. nov., an actinobacterium isolated from sea cucumber Holothuria edulis, and proposal of Iamiaceae fam. nov.. Int J Syst Evol Microbiol 59, 869–873. [View Article] [PubMed]
    [Google Scholar]
  12. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
     [Google Scholar]
  13. Matsumoto A., Kasai H., Matsuo Y., Omura S., Shizuri Y., Takahashi Y. J. ( 2009 ). Ilumatobacter fluminis gen. nov., sp. nov., a novel actinobacterium isolated from the sediment of an estuary. . J Gen Appl Microbiol 55, 201–205. [View Article] [PubMed]
    [Google Scholar]
  14. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. ( 1977 ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27, 104–117. [View Article]
    [Google Scholar]
  15. Oh H. M., Ahn C. Y., Lee J. W., Chon T. S., Choi K. H., Park Y. S. ( 2007 ). Community patterning and identification of predominant factors in algal bloom in Daechung reservoir (Korea) using artificial neural networks. . Ecol Modell 203, 109–118. [View Article]
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  17. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101 . . Newark, DE:: MIDI Inc;.
     [Google Scholar]
  18. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reverse-phased high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125–128. [View Article]
    [Google Scholar]
  19. Tarrand J. J., Gröschel D. H. M. ( 1982 ). Rapid, modified oxidase test for oxidase-variable bacterial isolates. . J Clin Microbiol 16, 772–774.[PubMed]
    [Google Scholar]
  20. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 4876–4882. [View Article] [PubMed]
    [Google Scholar]
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Aquihabitans daechungensis gen. nov., sp. nov., an actinobacterium isolated from reservoir water
Int J Syst Evol Microbiol 63, 2970 (2013); https://doi.org/10.1099/ijs.0.046060-0
/content/journal/ijsem/10.1099/ijs.0.046060-0
/content/journal/ijsem/10.1099/ijs.0.046060-0
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