1887

Abstract

A novel Gram-reaction-positive bacterium, strain CH22-21, was isolated from a water sample taken from Daechung Reservoir, Republic of Korea, during the late-blooming period of cyanobacteria. Cells of strain CH22-21 were non-motile, ciliated short rods that formed creamy-white colonies on half-strength modified R2A agar. Chemotaxonomic results showed menaquinone MK-9(H) as the predominant respiratory menaquinone, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside as major polar lipids, 16 : 1ω5, 16 : 0, 17 : 1ω8 and 18 : 1ω9 as major fatty acids, and a DNA G+C content of 71.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the strain formed a separate lineage within the order , showing similarity values of <92.3 % with its closest phylogenetic neighbours. The combined genotypic and phenotypic data showed that strain CH22-21 could be distinguished from all genera within the order and represented a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is CH22-21 ( = KCTC 19849 = JCM 17787).

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2013-08-01
2020-01-25
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