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Abstract

A Gram-stain-negative, non-spore-forming, strictly aerobic, non-flagellated, non-gliding, rod-shaped bacterial strain, designated SMS-12, was isolated from marine sand in a firth on the western coast of South Korea. Strain SMS-12 grew optimally at 25 °C, at pH 7.0–7.5 and in the absence of NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain SMS-12 fell within the clade comprising species of the genus , forming a coherent cluster with the type strain of , with which it exhibited the highest 16S rRNA gene sequence similarity value of 97.5 %. Levels of sequence similarity to the type strains of the other species of the genus and the other species used in the phylogenetic analysis were 93.3–96.4 % and <91.5 %, respectively. Strain SMS-12 contained MK-7 as the predominant menaquinone, and summed feature 3 (Cω7 and/or iso-C 2-OH), iso-C and iso-C 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and one unidentified aminophospholipid; sphingolipids were present. The DNA G+C content was 41.8 mol% and the mean DNA–DNA relatedness with KACC 14978 was 13 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain SMS-12 is separate from other species of the genus . On the basis of the data presented, strain SMS-12 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SMS-12 ( = KCTC 32111 = CCUG 62214).

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2013-08-01
2020-01-20
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