1887

Abstract

Two actinomycete strains, BK125 and BK199, isolated from a hay meadow soil sample were investigated to determine their taxonomic position using a polyphasic approach. The isolates produced greenish-yellow and light green aerial mycelium on oatmeal agar, respectively. They contained anteiso-C, iso-C and C as the major fatty acids, and MK-9 (H) and MK-9 (H) as the predominant isoprenoid quinones. Phylogenetic analysis of the 16S rRNA gene sequences showed that the isolates formed distinct phyletic lines towards the periphery of the subclade. Analysis of DNA–DNA relatedness between the two isolates showed that they belonged to different genomic species. The organisms were also distinguished from one another and from type strains of species classified in the subclade using a combination of genotypic and phenotypic properties. On the basis of these data, it is proposed that the isolates be assigned to the genus as sp. nov. and sp. nov. with isolates BK125 ( = KACC 20902 = CGMCC 4.5798) and BK199 ( = KACC 21003 = CGMCC 4.6824) as the respective type strains.

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2013-05-01
2019-10-23
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