A non-motile, Gram-stain-negative bacterium, designated R-HLS-17T, was isolated from the meltwater of Hailuogou Glacier located in Sichuan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Flavobacterium, with the closest relatives being Flavobacterium antarcticum JCM 12383T (95.5 % 16S rRNA gene sequence similarity), F. omnivorum JCM 11313T (95.0 %) and F. fryxellicola LMG 22022T (95.2 %). Growth occurred at 0–29 °C (optimum, 10–20 °C) and pH 6.0–8.5 (optimum, 7.0–8.0). The DNA G+C content was 46.5 mol%. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G, summed feature 9 (comprising iso-C17 : 1ω9c and/or 10-methyl C16 : 0), iso-C17 : 0 3-OH and iso-C15 : 0 3-OH. The predominant menaquinone was MK-6. Based on the genotypic and phenotypic characteristics, we propose that strain R-HLS-17T represents a novel species of the genus Flavobacterium, Flavobacterium noncentrifugens sp. nov. The type strain is R-HLS-17T ( = CGMCC 1.10076T = NBRC 108844T).
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Funding
This study was supported by the:
National Natural Science Foundation of China
(Award 31070001/C010101)
Knowledge Innovation Program of Chinese Academy of Sciences
(Award KSCXZ-YW-Z-0937)
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