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Abstract

A non-motile, Gram-stain-negative bacterium, designated R-HLS-17, was isolated from the meltwater of Hailuogou Glacier located in Sichuan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus , with the closest relatives being JCM 12383 (95.5 % 16S rRNA gene sequence similarity), JCM 11313 (95.0 %) and LMG 22022 (95.2 %). Growth occurred at 0–29 °C (optimum, 10–20 °C) and pH 6.0–8.5 (optimum, 7.0–8.0). The DNA G+C content was 46.5 mol%. The major cellular fatty acids were iso-C, iso-C G, summed feature 9 (comprising iso-Cω9 and/or 10-methyl C), iso-C 3-OH and iso-C 3-OH. The predominant menaquinone was MK-6. Based on the genotypic and phenotypic characteristics, we propose that strain R-HLS-17 represents a novel species of the genus , sp. nov. The type strain is R-HLS-17 ( = CGMCC 1.10076 = NBRC 108844).

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 31070001/C010101)
  • , Knowledge Innovation Program of Chinese Academy of Sciences , (Award KSCXZ-YW-Z-0937)
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2013-06-01
2021-02-26
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