RT Journal Article SR Electronic(1) A1 Saxena, Anjali A1 Anand, Shailly A1 Dua, Ankita A1 Sangwan, Naseer A1 Khan, Fazlurrahman A1 Lal, RupYR 2013 T1 Novosphingobium lindaniclasticum sp. nov., a hexachlorocyclohexane (HCH)-degrading bacterium isolated from an HCH dumpsite JF International Journal of Systematic and Evolutionary Microbiology, VO 63 IS Pt_6 SP 2160 OP 2167 DO https://doi.org/10.1099/ijs.0.045443-0 PB Microbiology Society, SN 1466-5034, AB A yellow-pigmented, Gram-negative, aerobic, non-motile, non-spore-forming, rod-shaped-bacterium, LE124T, was isolated from a hexachlorocyclohexane (HCH) dumpsite located in Lucknow, India. The type strain LE124T grew well with hexachlorocyclohexane as a sole carbon source, degrading it within 24 h of incubation. Phylogenetic analysis of strain LE124T showed highest 16S rRNA gene sequence similarity to Novosphingobium barchaimii LL02T (98.5 %), Novosphingobium panipatense SM16T (98.1 %), Novosphingobium soli CC-TPE-1T (97.9 %), Novosphingobium naphthalenivorans TUT562T (97.6 %), Novosphingobium mathurense SM117T (97.5 %) and Novosphingobium resinovorum NCIMB 8767T (97.5 %) and lower sequence similarity (<97 %) to all other members of the genus Novosphingobium . The DNA–DNA relatedness between strain LE124T and N. barchaimii LL02T and other related type strains was found to vary from 15 % to 45 % confirming that it represents a novel species. The genomic DNA G+C content of strain LE124T was 60.7 mol%. The predominant fatty acids were summed feature 8 (C18 : 1ω7c, 49.1 %), summed feature 3 (C16 : 1ω7c/C16 : 1ω6c, 19.9 %), C16 : 0 (6.7 %), C17 : 1ω6c (4.9 %) and a few hydroxyl fatty acids, C14 : 0 2-OH (9.4 %) and C16 : 0 2-OH (2.1 %). Polar lipids consisted mainly of phosphatidyldimethylethanolamine, phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, sphingoglycolipid and some unidentified lipids. The major respiratory quinone was ubiquinone Q-10. Spermidine was the major polyamine observed. Phylogenetic analysis, DNA–DNA hybridization, chemotaxonomic and phenotypic analysis support the conclusion that strain LE124T represents a novel species within the genus Novosphingobium for which we propose the name Novosphingbium lindaniclasticum sp. nov. The type strain is LE124T ( = CCM 7976T = DSM 25409T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.045443-0