1887

Abstract

A yellow-pigmented, Gram-negative, aerobic, non-motile, non-spore-forming, rod-shaped-bacterium, LE124, was isolated from a hexachlorocyclohexane (HCH) dumpsite located in Lucknow, India. The type strain LE124 grew well with hexachlorocyclohexane as a sole carbon source, degrading it within 24 h of incubation. Phylogenetic analysis of strain LE124 showed highest 16S rRNA gene sequence similarity to LL02 (98.5 %), SM16 (98.1 %), CC-TPE-1 (97.9 %), TUT562 (97.6 %), SM117 (97.5 %) and NCIMB 8767 (97.5 %) and lower sequence similarity (<97 %) to all other members of the genus . The DNA–DNA relatedness between strain LE124 and LL02 and other related type strains was found to vary from 15 % to 45 % confirming that it represents a novel species. The genomic DNA G+C content of strain LE124 was 60.7 mol%. The predominant fatty acids were summed feature 8 (Cω7, 49.1 %), summed feature 3 (Cω7/Cω6, 19.9 %), C (6.7 %), Cω6 (4.9 %) and a few hydroxyl fatty acids, C 2-OH (9.4 %) and C 2-OH (2.1 %). Polar lipids consisted mainly of phosphatidyldimethylethanolamine, phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, sphingoglycolipid and some unidentified lipids. The major respiratory quinone was ubiquinone Q-10. Spermidine was the major polyamine observed. Phylogenetic analysis, DNA–DNA hybridization, chemotaxonomic and phenotypic analysis support the conclusion that strain LE124 represents a novel species within the genus for which we propose the name sp. nov. The type strain is LE124 ( = CCM 7976 = DSM 25409).

Funding
This study was supported by the:
  • University of Delhi Department of Science and Technology (DU/DST)
  • Department of Biotechnology (DBT)
  • Government of India
  • National Bureau of Agriculturally Important Micro-organisms (NBAIM)
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2013-06-01
2024-03-29
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