1887

Abstract

A novel α-amylase/pullulanase-producing bacterium, designated strain GST4, was isolated from samples collected from the wastewater of a cassava starch factory in Nanning, Guangxi Autonomous Region, southern China. Cells of strain GST4 were rod-shaped bacilli containing ellipsoidal terminal spores and found to be Gram-reaction-positive, aerobic, motile, oxidase-positive, catalase-negative and formed light yellow colonies on agar plates. Strain GST4 was able to grow at pH 4.5–8.5 (optimum at pH 5.5), temperatures ranging from 20 to 42 °C (optimum at 37 °C) and salt concentrations of 0–1 % (w/v) NaCl (optimum at 0.5 %, w/v) on R2A medium. Strain GST4 grew heterotrophically on complex carbon substrates and chemolithoautotrophically on inorganic sulfur compounds, as demonstrated by growth on sodium thiosulfate and sulfite as sole electron donors. It can reduce nitrate and nitrite. Strain GST4 contained iso-C and anteiso-C as the major cellular fatty acids and menaquinone 7 (MK-7) as the major respiratory quinone. The cell-wall peptidoglycan was of type A1γ. The genomic DNA G+C content of strain GST4 was 53.7 mol%. Physiological and chemotaxonomic characteristics combined with phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GST4 was a member of the genus and most closely related to DSM 18773 and DSM 18389 with 97.3 and 94.5 % sequence similarity, respectively. The DNA–DNA relatedness values between strain GST4 and DSM 18773, and strain GST4 and DSM 18389 were 44.0 and 60.4 %, respectively. The new isolate differed from those species of the genus in that it has peritrichous flagella for motility. Based on the evidence obtained from this study, strain GST4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GST4 ( = CGMCC 1.12170 = DSM 25748).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.045351-0
2013-09-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/9/3138.html?itemId=/content/journal/ijsem/10.1099/ijs.0.045351-0&mimeType=html&fmt=ahah

References

  1. Atlas R. M.. ( 1993;). Handbook of Microbiological Media. Boca Raton, FL:: CRC Press;.
    [Google Scholar]
  2. Baek S. H., Cui Y. S., Kim S. C., Cui C. H., Yin C. R., Lee S. T., Im W. T.. ( 2011;). Tumebacillus ginsengisoli sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 61:, 1715–1719. [CrossRef][PubMed]
    [Google Scholar]
  3. Buck J. D.. ( 1982;). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. . Appl Environ Microbiol 44:, 992–993.[PubMed]
    [Google Scholar]
  4. Claus D., Berkeley R. C. W.. ( 1986;). Genus Bacillus Cohn 1872. . In Bergey’s Manual of Systematic Bacteriology, vol. 2, pp. 1105–1139. Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G... Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  6. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  7. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  9. Kluge A. G., Farris J. S.. ( 1969;). Quantitative phyletics and the evolution of anurans. . Syst Zool 18:, 1–32. [CrossRef]
    [Google Scholar]
  10. Lane D. J.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M... Chichester:: Wiley;.
    [Google Scholar]
  11. Marmur J., Doty P.. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef][PubMed]
    [Google Scholar]
  12. Rhuland L. E., Work E., Denman R. F., Hoare D. S.. ( 1955;). The behavior of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. . J Am Chem Soc 77:, 4844–4846. [CrossRef]
    [Google Scholar]
  13. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  14. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  15. Steven B., Briggs G., McKay C. P., Pollard W. H., Greer C. W., Whyte L. G.. ( 2007;). Characterization of the microbial diversity in a permafrost sample from the Canadian high Arctic using culture-dependent and culture-independent methods. . FEMS Microbiol Ecol 59:, 513–523. [CrossRef][PubMed]
    [Google Scholar]
  16. Steven B., Chen M. Q., Greer C. W., Whyte L. G., Niederberger T. D.. ( 2008;). Tumebacillus permanentifrigoris gen. nov., sp. nov., an aerobic, spore-forming bacterium isolated from Canadian high Arctic permafrost. . Int J Syst Evol Microbiol 58:, 1497–1501. [CrossRef][PubMed]
    [Google Scholar]
  17. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.045351-0
Loading
/content/journal/ijsem/10.1099/ijs.0.045351-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error