1887

Abstract

A Gram-staining-negative, yellow-pigmented bacterial strain, motile by gliding, designated HME7102, was isolated from lagoon water in Korea. The phylogenetic tree based on 16S rRNA gene sequences showed that strain HME7102 formed a distinct lineage within the genus . Strain HME7102 was closely related to NJ-26 (94.5 %), LW30 (94.4 %) and LMG 21477 (94.2 %). The major fatty acids of strain HME7102 were iso-C, iso-C G, iso-C 3-OH and iso-C 3-OH. The major polyamine was homospermidine and the major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and two unidentified polar lipids. The DNA G+C content of strain HME7102 was 36.5 mol%. On the basis of the evidence presented in this study, strain HME7102 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HME7102 ( = KCTC 23618 = CECT 7955). Emended descriptions of , and are also proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.045286-0
2013-11-01
2020-01-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/11/3937.html?itemId=/content/journal/ijsem/10.1099/ijs.0.045286-0&mimeType=html&fmt=ahah

References

  1. Atlas R. M.. ( 2010;). Handbook of Microbiological Media, , 4th edn.. Boca Raton:: CRC Press;. [CrossRef]
    [Google Scholar]
  2. Bergey D. H., Harrison F. C., Breed R. S., Hammer B. W., Huntoon F. M.. ( 1923;). Bergeys Manual of Determinative Bacteriology. Baltimore:: The Williams & Wilkins Co;.
    [Google Scholar]
  3. Bernardet J.-F., Bowman J. P.. ( 2006;). The genus Flavobacterium. . In The Prokaryotes: A Handbook on the Biology of Bacteria, , 3rd edn., vol. 7, pp. 481–531. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E... New York:: Springer;.
    [Google Scholar]
  4. Bernardet J. F., Bowman J. P.. ( 2011;). Genus I. Flavobacterium Bergey et al. 1923. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 112–154. Edited by Whitman W... Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  5. Bernardet J. F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P.. ( 1996;). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). . Int J Syst Evol Microbiol 46:, 128–148.
    [Google Scholar]
  6. Bernardet J. F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  7. Busse H.-J., Bunka S., Hensel A., Lubitz W.. ( 1997;). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. . Int J Syst Bacteriol 47:, 698–708. [CrossRef]
    [Google Scholar]
  8. Cho J. C., Giovannoni S. J.. ( 2003;). Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria.. Int J Syst Evol Microbiol 53:, 1031–1036. [CrossRef][PubMed]
    [Google Scholar]
  9. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  10. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  11. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  12. Horne A. J., Goldman C. R.. ( 1994;). Limnology, , 2nd edn.. New York:: MaGraw-Hill;.
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  14. MacFaddin J. F.. ( 1980;). Biochemical Tests for Identification of Medical Bacteria, , 2nd edn.. Baltimore, MD:: Williams & Wilkins;.
    [Google Scholar]
  15. Mesbah M., Whitman W. B.. ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr 479:, 297–306. [CrossRef][PubMed]
    [Google Scholar]
  16. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  17. Qu J. H., Li H. F., Yang J. S., Yuan H. L.. ( 2008;). Flavobacterium cheniae sp. nov., isolated from sediment of a eutrophic reservoir. . Int J Syst Evol Microbiol 58:, 2186–2190. [CrossRef][PubMed]
    [Google Scholar]
  18. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  19. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. , MIDI Technical Note 101. Newark, DE:: MIDI Inc.;
    [Google Scholar]
  20. Scherer P., Kneifel H.. ( 1983;). Distribution of polyamines in methanogenic bacteria. . J Bacteriol 154:, 1315–1322.[PubMed]
    [Google Scholar]
  21. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  22. Van Trappen S., Mergaert J., Swings J.. ( 2003;). Flavobacterium gelidilacus sp. nov., isolated from microbial mats in Antarctic lakes. . Int J Syst Evol Microbiol 53:, 1241–1245. [CrossRef][PubMed]
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  24. Xiao Y. P., Hui W., Lee J. S., Lee K. C., Quan Z. X.. ( 2011;). Flavobacterium dongtanense sp. nov., isolated from the rhizosphere of a wetland reed. . Int J Syst Evol Microbiol 61:, 343–346. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.045286-0
Loading
/content/journal/ijsem/10.1099/ijs.0.045286-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error