1887

Abstract

A Gram-negative, motile, non-spore-forming rod, designated NS12, was isolated from macerated petioles of sp. after enrichment with oxalate. On the basis of 16S rRNA gene sequence similarity, strain NS12 was phylogenetically related to the genera and in the class . Strain NS12 was most closely related to BR 5401 and ORS 571 (98.3 and 97.3 % 16S rRNA gene sequence similarity, respectively). Membership of the genus was excluded by phenotypic characterization. The whole-cell fatty acid compositions of the isolate was typical of members of the genus with Cω7, cyclo-Cω8, 11-methyl-Cω7 and C as the main components. The results of DNA–DNA hybridization and physiological tests allowed the genotypic and phenotypic differentiation of strain NS12 from the two members of the genus . Therefore it is concluded that the isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NS12 ( = DSM 18749 = CCM 7897). The description of the genus is also emended.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.045229-0
2013-04-01
2022-01-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/4/1505.html?itemId=/content/journal/ijsem/10.1099/ijs.0.045229-0&mimeType=html&fmt=ahah

References

  1. Arun A. B., Schumann P., Chu H.-I., Tan C.-C., Chen W.-M., Lai W.-A., Kämpfer P., Shen F.-T., Rekha P. D. & other authors ( 2008 ). Pseudoxanthobacter soli gen. nov., sp. nov., a nitrogen-fixing alphaproteobacterium isolated from soil. . Int J Syst Evol Microbiol 58, 15711575. [View Article] [PubMed]
    [Google Scholar]
  2. Buck J. D. ( 1982 ). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. . Appl Environ Microbiol 44, 992993. [View Article]
    [Google Scholar]
  3. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. ( 1977 ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81, 461466. [View Article] [PubMed]
    [Google Scholar]
  4. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221230. [View Article] [PubMed]
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  6. De Soete G. ( 1983 ). A least squares algorithm for fitting additive trees to proximity data. . Psychometrika 48, 621626. [View Article]
    [Google Scholar]
  7. de Souza Moreira F. M., Cruz L., Miana de Faria S., Marsh T., Martínez-Romero E., de Oliveira Pedrosa F., Pitard R. M., Young J. P. W. ( 2006 ). Azorhizobium doebereinerae sp. nov. microsymbiont of Sesbania virgata (Caz.) Pers. . Syst Appl Microbiol 29, 197206. [View Article] [PubMed]
    [Google Scholar]
  8. Doronina N. V., Trotsenko Y. A. ( 2003 ). Reclassification of ‘Blastobacter viscosus’ 7d and ‘Blastobacter aminooxidans’ 14a as Xanthobacter viscosus sp. nov. and Xanthobacter aminoxidans sp. nov.. Int J Syst Evol Microbiol 53, 179182. [View Article] [PubMed]
    [Google Scholar]
  9. Dreyfus B., Garcia J. L., Gillis M. ( 1988 ). Characterization of Azorhizobium caulinodans gen. nov., sp. nov., a stem-nodulating nitrogen-fixing bacterium isolated from Sesbania rastrata . . Int J Syst Bacteriol 38, 8998. [View Article]
    [Google Scholar]
  10. Embley T. M., Wait R. ( 1994 ). Structural lipids of Eubacteria . . In Chemical Methods in Prokaryotic Systematics, pp. 141147. Edited by Goodfellow M., O’Donnell A. G. . New York:: John Wiley and Sons;.
    [Google Scholar]
  11. Garrity G. M., Bell J. A., Lilburn T. ( 2005 ). Family VIII. Hyphomicrobiaceae Babudieri 1950, 589. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, p. 476. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. . New York:: Springer;.
    [Google Scholar]
  12. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. ( 1997 ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47, 11291133. [View Article] [PubMed]
    [Google Scholar]
  13. Hugh R., Leifson E. ( 1953 ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram negative bacteria.. J Bacteriol 66, 2426. [View Article] [PubMed]
    [Google Scholar]
  14. Huisman G. W., Siegele D., Zambrano M. M., Kolter R. ( 1996 ). Morphological and physiological changes during stationary phase. . In Escherichia coli and Salmonella. Cellular and Molecular Biology, , 2nd edn., vol. 1, pp. 16721682. Edited by Neidhardt F. C. . Washington, D.C.:: American Society for Microbiology;.
    [Google Scholar]
  15. Huss V. A. R., Festl H., Schleifer K.-H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [View Article] [PubMed]
    [Google Scholar]
  16. Hwang C. Y., Cho B. C. ( 2008 ). Cohaesibacter gelatinilyticus gen. nov., a marine bacterium that forms a distinct branch in the order Rhizobiales, and proposal of Cohaesibacteraceae fam. nov.. Int J Syst Evol Microbiol 58, 267277. [View Article] [PubMed]
    [Google Scholar]
  17. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 9891005. [View Article]
    [Google Scholar]
  18. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  19. Lang E., Swiderski J., Stackebrandt E., Schumann P., Spröer C., Sahin N. ( 2008 ). Description of Ancylobacter oerskovii sp. nov. and two additional strains of Ancylobacter polymorphus . . Int J Syst Evol Microbiol 58, 19972002. [View Article] [PubMed]
    [Google Scholar]
  20. Lee K. B., Liu C. T., Anzai Y., Kim H., Aono T., Oyaizu H. ( 2005 ). The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov.. Int J Syst Evol Microbiol 55, 19071919. [View Article] [PubMed]
    [Google Scholar]
  21. Malik K. A. ( 1988 ). A new freeze-drying method for the preservation of nitrogen-fixing and other fragile bacteria. . J Microbiol Methods 8, 259271. [View Article]
    [Google Scholar]
  22. Minnikin D. E., Collins M. D., Goodfellow M. ( 1979 ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47, 8795. [View Article]
    [Google Scholar]
  23. Reding H. K. ( 1991 ). Ecological, physiological, and taxonomical studies of Xanthobacter strains isolated from the roots of wetland rice. . Dissertation, University of Georgia;, Athens, USA:.
    [Google Scholar]
  24. Reding H. K., Croes G. L., Dijkhuizen L., Wiegel J. ( 1992 ). Emendation of Xanthobacter flavus as a motile species. . Int J Syst Bacteriol 42, 309311. [View Article] [PubMed]
    [Google Scholar]
  25. Sahin N. ( 2003 ). Oxalotrophic bacteria. . Res Microbiol 154, 399407. [View Article] [PubMed]
    [Google Scholar]
  26. Sahin N. ( 2005 ). Isolation and characterization of a diazotrophic, oxalate-oxidizing bacterium from sour grass (Oxalis pes-caprae L.). . Res Microbiol 156, 452456. [View Article] [PubMed]
    [Google Scholar]
  27. Sahin N., Gökler I., Tamer A. Ü. ( 2002 ). Isolation, characterization and numerical taxonomy of novel oxalate-oxidizing bacteria. . J Microbiol 40, 109118.
    [Google Scholar]
  28. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  29. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  30. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607655. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg Washington N. R. . DC:: American Society for Microbiology;.
    [Google Scholar]
  31. So R. B., Ladha J. K., Young J. P. W. ( 1994 ). Photosynthetic symbionts of Aeschynomene spp. form a cluster with bradyrhizobia on the basis of fatty acid and rRNA analyses. . Int J Syst Bacteriol 44, 392403. [View Article] [PubMed]
    [Google Scholar]
  32. Somvanshi V. S., Lang E., Sträubler B., Spröer C., Schumann P., Ganguly S., Saxena A. K., Stackebrandt E. ( 2006 ). Providencia vermicola sp. nov., isolated from infective juveniles of the entomopathogenic nematode Steinernema thermophilum . . Int J Syst Evol Microbiol 56, 629633. [View Article] [PubMed]
    [Google Scholar]
  33. Stanier R. Y., Palleroni N. J., Doudoroff M. ( 1966 ). The aerobic pseudomonads: a taxonomic study. . J Gen Microbiol 43, 159271. [View Article] [PubMed]
    [Google Scholar]
  34. Stewart W. D. P., Fitzgerald G. P., Burris R. H. ( 1967 ). In situ studies on N2 fixation using the acetylene reduction technique. . Proc Natl Acad Sci U S A 58, 20712078. [View Article] [PubMed]
    [Google Scholar]
  35. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  36. Wiegel J. K. W. ( 2005 ). Genus XX. Xanthobacter Wiegel, Wilke, Baumgarten, Opitz and Schlegel 1978, 573AL . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, pp. 555566. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. . New York:: Springer;. [View Article]
    [Google Scholar]
  37. Wiegel J. K. W. ( 2006 ). The genus Xanthobacter . . In The Prokaryotes, , 3rd edn., vol. 5, pp. 290314. Edited by Dworkin M., Falkow S., Schleifer E. K.-H., Stackebrandt E. . New York:: Springer;. [View Article]
    [Google Scholar]
  38. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K.-H., Ludwig W., Glöckner F. O., Rosselló-Móra R. ( 2008 ). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. . Syst Appl Microbiol 31, 241250. [View Article] [PubMed]
    [Google Scholar]
  39. Yoon J.-H., Kang S.-J., Park S., Oh T.-K. ( 2007 ). Devosia insulae sp. nov., isolated from soil, and emended description of the genus Devosia . . Int J Syst Evol Microbiol 57, 13101314. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.045229-0
Loading
/content/journal/ijsem/10.1099/ijs.0.045229-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error