1887

Abstract

A bacterium, HKU30, was isolated from the infected tissue of a patient with wound infection after puncture by a fish fin. Cells are facultative anaerobic, non-spore-forming, non-motile, Gram-positive cocci arranged in chains. Colonies were non-haemolytic. The strain was catalase, oxidase, urease and Voges–Proskauer test negative. It reacted with Lancefield’s group G antisera and was resistant to optochin. It grew on bile aesculin agar and in 5 % NaCl. It was unidentified by three commercial identification systems. 16S rRNA gene sequence analysis indicated that the bacterium shared 98.2, 97.7, 97.4 and 97.1 % nucleotide identities with , , and , respectively. The DNA G+C content was 35.6±0.9 mol% (mean±). In view of the occupational exposure of the patient, an epidemiological study was performed to isolate the bacterium from marine fish. Two strains, with similar phenotypic and genotypic characteristics to those of HKU30, were isolated from a three-lined tongue sole () and an olive flounder () respectively. Phylogenetic analysis of four additional housekeeping genes, , , and , showed that the three isolates formed a distinct branch among known species of the genus , being most closely related to (CCUG 39954). DNA–DNA hybridization demonstrated 53.8 % DNA relatedness between the three isolates and related species of the genus . A novel species, sp. nov., is proposed. The type strain is HKU30 ( = DSM 26014 = CECT 8154).

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2013-07-01
2019-10-18
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