sp. nov., a slowly growing non-chromogenic species related to , isolated from a human clinical specimen Free

Abstract

A previously undescribed, slowly growing, non-chromogenic strain (299) was isolated from the sputum sample of a patient with a symptomatic pulmonary infection. Phenotypically, strain 299 was generally similar to DSM 45576 and ATCC 23292. The 16S rRNA gene sequence of strain 299 was similar to that of DSM 45576 (GenBank accession no. AY734996, 99.5 % similarity); however, it differed substantially from that of ATCC 23292 (X88924, 98.2 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 299 clustered together with DSM 45576 and ATCC 23292, supported by high bootstrapping values (99 %). Unique mycolic acid profiles and phylogenetic analysis based on two different chronometer molecules, the and genes, strongly supported the taxonomic status of this strain as representing a distinct species. These data support the conclusion that strain 299 represents a novel mycobacterial species, for which the name sp. nov. is proposed. The type strain is 299 ( = DSM 45575 = KCTC 19818).

Funding
This study was supported by the:
  • National Research Foundation of Korea (NRF) (Award 2012R1A1A20414)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.045070-0
2013-06-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/6/2301.html?itemId=/content/journal/ijsem/10.1099/ijs.0.045070-0&mimeType=html&fmt=ahah

References

  1. Adékambi T., Colson P., Drancourt M. ( 2003 ). rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria. . J Clin Microbiol 41, 56995708. [View Article] [PubMed]
    [Google Scholar]
  2. Butler W. R., Thibert L., Kilburn J. O. ( 1992 ). Identification of Mycobacterium avium complex strains and some similar species by high-performance liquid chromatography. . J Clin Microbiol 30, 26982704.[PubMed]
    [Google Scholar]
  3. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  4. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406. [View Article]
    [Google Scholar]
  5. Jukes T. H., Cantor C. R. (editors) ( 1969 ). Evolution of Protein Molecules. New York:: Academic Press;.
    [Google Scholar]
  6. Kent P. T., Kubica G. P. ( 1985 ). Public Health Mycobacteriology: A Guide for the Level III Laboratory. Atlanta:: Centers for Disease Control and Prevention;.
    [Google Scholar]
  7. Kim B. J., Lee S. H., Lyu M. A., Kim S. J., Bai G. H., Chae G. T., Kim E. C., Cha C. Y., Kook Y. H. ( 1999 ). Identification of mycobacterial species by comparative sequence analysis of the RNA polymerase gene (rpoB). . J Clin Microbiol 37, 17141720.[PubMed]
    [Google Scholar]
  8. Kim H., Kim S. H., Shim T. S., Kim M. N., Bai G. H., Park Y. G., Lee S. H., Chae G. T., Cha C. Y. et al. ( 2005 ). Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65). . Int J Syst Evol Microbiol 55, 16491656. [View Article] [PubMed]
    [Google Scholar]
  9. Kim H. J., Mun H. S., Kim H., Oh E. J., Ha Y. J., Bai G. H., Park Y. G., Cha C. Y., Kook Y. H., Kim B.-J. ( 2006 ). Differentiation of mycobacterial species by hsp65 duplex PCR followed by duplex-PCR-based restriction analysis and direct sequencing. . J Clin Microbiol 44, 38553862. [View Article] [PubMed]
    [Google Scholar]
  10. Kim B. J., Jeong J., Lee S. H., Kim S. R., Yu H. K., Park Y. G., Kim K. J., Kook Y. H., Kim B.-J. ( 2012 ). Mycobacterium koreense sp. nov., a slowly growing non-chromogenic species closely related to Mycobacterium triviale . . Int J Syst Evol Microbiol 62, 12891295. [View Article] [PubMed]
    [Google Scholar]
  11. Kumar S., Nei M., Dudley J., Tamura K. ( 2008 ). mega: a biologist-centric software for evolutionary analysis of DNA and protein sequences. . Brief Bioinform 9, 299306. [View Article] [PubMed]
    [Google Scholar]
  12. Magee J. G., Ward A. C. ( 2011 ). Genus I Mycobacterium. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 5, pp. 312375. Edited by Goodfellow M. . New York:: Springer;.
    [Google Scholar]
  13. Minninkin D. ( 1988 ). Isolation and Purification of Mycobacterial Wall Lipids. Chichester:: Wiley;.
    [Google Scholar]
  14. Pérez E., Constant P., Lemassu A., Laval F., Daffé M., Guilhot C. ( 2004 ). Characterization of three glycosyltransferases involved in the biosynthesis of the phenolic glycolipid antigens from the Mycobacterium tuberculosis complex. . J Biol Chem 279, 4257442583. [View Article] [PubMed]
    [Google Scholar]
  15. Rogall T., Flohr T., Böttger E. C. ( 1990 ). Differentiation of Mycobacterium species by direct sequencing of amplified DNA. . J Gen Microbiol 136, 19151920.[PubMed] [CrossRef]
    [Google Scholar]
  16. Rosselló-Mora R., Amann R. ( 2001 ). The species concept for prokaryotes. . FEMS Microbiol Rev 25, 3967. [View Article] [PubMed]
    [Google Scholar]
  17. Roth A., Fischer M., Hamid M. E., Michalke S., Ludwig W., Mauch H. ( 1998 ). Differentiation of phylogenetically related slowly growing mycobacteria based on 16S–23S rRNA gene internal transcribed spacer sequences. . J Clin Microbiol 36, 139147.[PubMed]
    [Google Scholar]
  18. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  19. Sambrook J., Russell D. W. ( 2001 ). Molecular Cloning: a Laboratory Manual, , 3rd. edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  20. Springer B., Stockman L., Teschner K., Roberts G. D., Böttger E. C. ( 1996 ). Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods. . J Clin Microbiol 34, 296303.[PubMed]
    [Google Scholar]
  21. Turenne C. Y., Tschetter L., Wolfe J., Kabani A. ( 2001 ). Necessity of quality-controlled 16S rRNA gene sequence databases: identifying nontuberculous Mycobacterium species. . J Clin Microbiol 39, 36373648. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.045070-0
Loading
/content/journal/ijsem/10.1099/ijs.0.045070-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed