1887

Abstract

A previously undescribed, slowly growing, non-chromogenic strain (299) was isolated from the sputum sample of a patient with a symptomatic pulmonary infection. Phenotypically, strain 299 was generally similar to DSM 45576 and ATCC 23292. The 16S rRNA gene sequence of strain 299 was similar to that of DSM 45576 (GenBank accession no. AY734996, 99.5 % similarity); however, it differed substantially from that of ATCC 23292 (X88924, 98.2 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 299 clustered together with DSM 45576 and ATCC 23292, supported by high bootstrapping values (99 %). Unique mycolic acid profiles and phylogenetic analysis based on two different chronometer molecules, the and genes, strongly supported the taxonomic status of this strain as representing a distinct species. These data support the conclusion that strain 299 represents a novel mycobacterial species, for which the name sp. nov. is proposed. The type strain is 299 ( = DSM 45575 = KCTC 19818).

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2013-06-01
2019-10-20
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