1887

Abstract

Two novel violet-pigmented, Gram-negative, rod-shaped, non-motile bacteria, designated strains M4-16 and M4-9, were isolated from sediment from an Arctic glacier. The predominant fatty acids of both strains were Cω7 and/or Cω6 (summed feature 3), C, C and Cω7 and/or Cω6 (summed feature 8) and both strains contained ubiquinone-8 as the respiratory quinone. The polar lipids consisted of phosphatidylethanolamine, two unidentified phospholipids and one unidentified aminolipid. 16S rRNA gene sequence analysis indicated that strains M4-16 and M4-9 were members of the genus and closely related to ATCC 33051 with pairwise sequence similarity of 98.9 %. The DNA–DNA relatedness between strains M4-9 and M4-16 was 92.5 %, while strains M4-9 and M4-16 had DNA–DNA relatedness values of 21.5 and 18.2 %, respectively, with JCM 9044. The RAPD-PCR banding patterns of strains M4-9 and M4-16 were similar but differed from that of JCM 9044. Based on data from the current polyphasic study, strains M4-16 and M4-9 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is M4-16 ( = CIP 1103011 = MTCC 11351).

Funding
This study was supported by the:
  • , National Centre for Antarctic and Ocean Research, Goa
  • , CSIR Network Project on Exploitation of India’s rich microbial diversity , (Award NWP0006)
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2013-08-01
2020-07-08
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