RT Journal Article SR Electronic(1) A1 Sheu, Shih-Yi A1 Sheu, Der-Shyan A1 Sheu, Fu-Sian A1 Chen, Wen-MingYR 2013 T1 Gemmobacter tilapiae sp. nov., a poly-β-hydroxybutyrate-accumulating bacterium isolated from a freshwater pond JF International Journal of Systematic and Evolutionary Microbiology, VO 63 IS Pt_4 SP 1550 OP 1556 DO https://doi.org/10.1099/ijs.0.044735-0 PB Microbiology Society, SN 1466-5034, AB A novel bacterium, designated strain Ruye-53T, was isolated from a freshwater pond used to rear tilapiine cichlid fish in Taiwan. The taxonomic position of the novel strain was determined using a polyphasic approach. Strain Ruye-53T was Gram-reaction-negative, aerobic, cream–white coloured, rod-shaped, non-motile and poly-β-hydroxybutyrate-accumulating. Growth occurred at 10–37 °C (optimum between 20 and 25 °C), at pH 6.0–9.0 (optimum between pH 8.0 and pH 9.0) and with 0–1 % (w/v) NaCl (optimum 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Ruye-53T belonged to the genus Gemmobacter and that its most closely related neighbour was Gemmobacter aquatilis DSM 3857T (97.6 % sequence similarity). The novel strain’s predominant fatty acid was C18 : 1ω7c, its major respiratory quinone was Q-10 and its genomic DNA G+C content was 61.2 mol%. The polar lipid profile consisted of a mixture of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one uncharacterized aminolipid and four uncharacterized phospholipids. The DNA–DNA relatedness value between strain Ruye-53T and Gemmobacter aquatilis DSM 3857T was about 45.5 % (48.2±0.4 % in the reciprocal experiment). On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Ruye-53T represents a novel species in the genus Gemmobacter , for which the name Gemmobacter tilapiae sp. nov. is proposed. The type strain is Ruye-53T ( = BCRC 80261T  = KCTC 23310T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.044735-0