1887

Abstract

A novel anaerobic, mesophilic, amino-acid-fermenting bacterium, designated strain CL-84, was isolated from the swine intestinal tract on mucin-based media. Cells were curved rods (0.8–1.2×3.5–5.0 µm), stained Gram-negative and were non-motile with no evidence of spores. Strain CL-84 produced acetate, propionate, formate and butyrate as the end products of metabolism when grown on serine. Optimum growth occurred at 39 °C and pH 6.5. The major cellular fatty acids were iso-C, iso-C 3-OH, iso-C and C, distinguishing strain CL-84 from closely related species. The DNA G+C content of strain CL-84 was 55.1 mol%. 16S rRNA gene sequence analysis showed that strain CL-84 shared 90–95 % similarity with characterized genera within the phylum , family Phylogenetic analysis showed that strain CL-84 was related to, but distinct from, Based on these findings, we propose that strain CL-84 represents a novel species of the genus . We further propose the name sp. nov. be designated for this species. The type strain is CL-84 ( = DSM 25858 = CCUG 62631). An emended description of the genus is provided.

Funding
This study was supported by the:
  • Agricultural Research Service
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.044719-0
2013-06-01
2022-05-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/6/1960.html?itemId=/content/journal/ijsem/10.1099/ijs.0.044719-0&mimeType=html&fmt=ahah

References

  1. Allen A., Hutton D. A., Pearson J. P. ( 1998 ). The MUC2 gene product: a human intestinal mucin. . Int J Biochem Cell Biol 30, 797801. [View Article] [PubMed]
    [Google Scholar]
  2. Allen H. K., Cloud-Hansen K. A., Wolinski J. M., Guan C., Greene S., Lu S., Boeyink M., Broderick N. A., Raffa K. F., Handelsman J. ( 2009 ). Resident microbiota of the gypsy moth midgut harbors antibiotic resistance determinants. . DNA Cell Biol 28, 109117. [View Article] [PubMed]
    [Google Scholar]
  3. Allison M. J., Mayberry W. R., McSweeney C. S., Stahl D. A. ( 1992 ). Synergistes jonesii, gen. nov., sp. nov.: a rumen bacterium that degrades toxic pyridinediols. . Syst Appl Microbiol 15, 522529. [View Article]
    [Google Scholar]
  4. Bradshaw D. J., Homer K. A., Marsh P. D., Beighton D. ( 1994 ). Metabolic cooperation in oral microbial communities during growth on mucin. . Microbiology 140, 34073412. [View Article] [PubMed]
    [Google Scholar]
  5. Buckel W., Dorn U., Semmler R. ( 1981 ). Glutaconate CoA-transferase from Acidaminococcus fermentans . . Eur J Biochem 118, 315321. [View Article] [PubMed]
    [Google Scholar]
  6. Cole J. R., Wang Q., Cardenas E., Fish J., Chai B., Farris R. J., Kulam-Syed-Mohideen A. S., McGarrell D. M., Marsh T. et al. ( 2009 ). The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. . Nucleic Acids Res 37 (Database issue), D141D145. [View Article] [PubMed]
    [Google Scholar]
  7. Dale J. W., Greenaway P. J. ( 1985 ). Preparation of chromosomal DNA from E. coli . . Methods Mol Biol 2, 197200.[PubMed]
    [Google Scholar]
  8. Downes J., Sutcliffe I., Tanner A. C., Wade W. G. ( 2005 ). Prevotella marshii sp. nov. and Prevotella baroniae sp. nov., isolated from the human oral cavity. . Int J Syst Evol Microbiol 55, 15511555. [View Article] [PubMed]
    [Google Scholar]
  9. Downes J., Vartoukian S. R., Dewhirst F. E., Izard J., Chen T., Yu W. H., Sutcliffe I. C., Wade W. G. ( 2009 ). Pyramidobacter piscolens gen. nov., sp. nov., a member of the phylum ‘Synergistetes’ isolated from the human oral cavity. . Int J Syst Evol Microbiol 59, 972980. [View Article] [PubMed]
    [Google Scholar]
  10. Finnie I. A., Dwarakanath A. D., Taylor B. A., Rhodes J. M. ( 1995 ). Colonic mucin synthesis is increased by sodium butyrate. . Gut 36, 9399. [View Article] [PubMed]
    [Google Scholar]
  11. Ganesan A., Chaussonnerie S., Tarrade A., Dauga C., Bouchez T., Pelletier E., Le Paslier D., Sghir A. ( 2008 ). Cloacibacillus evryensis gen. nov., sp. nov., a novel asaccharolytic, mesophilic, amino-acid-degrading bacterium within the phylum ‘Synergistetes’, isolated from an anaerobic sludge digester. . Int J Syst Evol Microbiol 58, 20032012. [View Article] [PubMed]
    [Google Scholar]
  12. Greene R. A., Blum E. F., DeCoro C. T., Fairchild R. B., Kaplan M. T., Landau J. L., Sharp T. R. ( 1951 ). Rapid methods for the detection of motility. . J Bacteriol 62, 347.[PubMed]
    [Google Scholar]
  13. Hatayama H., Iwashita J., Kuwajima A., Abe T. ( 2007 ). The short chain fatty acid, butyrate, stimulates MUC2 mucin production in the human colon cancer cell line, LS174T. . Biochem Biophys Res Commun 356, 599603. [View Article] [PubMed]
    [Google Scholar]
  14. Horz H. P., Citron D. M., Warren Y. A., Goldstein E. J., Conrads G. ( 2006 ). Synergistes group organisms of human origin. . J Clin Microbiol 44, 29142920. [View Article] [PubMed]
    [Google Scholar]
  15. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. et al. ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [View Article] [PubMed]
    [Google Scholar]
  16. Marchandin H., Damay A., Roudière L., Teyssier C., Zorgniotti I., Dechaud H., Jean-Pierre H., Jumas-Bilak E. ( 2010 ). Phylogeny, diversity and host specialization in the phylum Synergistetes with emphasis on strains and clones of human origin. . Res Microbiol 161, 91100. [View Article] [PubMed]
    [Google Scholar]
  17. Martens E. C., Chiang H. C., Gordon J. I. ( 2008 ). Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. . Cell Host Microbe 4, 447457. [View Article] [PubMed]
    [Google Scholar]
  18. Nataro J. P. ( 2005 ). Colonization of Mucosal Surfaces. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  19. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. ( 2007 ). SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. . Nucleic Acids Res 35, 71887196. [View Article] [PubMed]
    [Google Scholar]
  20. Salanitro J. P., Muirhead P. A. ( 1975 ). Quantitative method for the gas chromatographic analysis of short-chain monocarboxylic and dicarboxylic acids in fermentation media. . Appl Microbiol 29, 374381.[PubMed]
    [Google Scholar]
  21. Schrager J. ( 1970 ). The chemical composition and function of gastrointestinal mucus. . Gut 11, 450456. [View Article] [PubMed]
    [Google Scholar]
  22. Stackebrandt E., Goodfellow M. ( 1991 ). Nucleic Acid Techniques in Bacterial Systematics. New York:: Wiley;.
    [Google Scholar]
  23. Stanton T. B., Lebo D. F. ( 1988 ). Treponema hyodysenteriae growth under various culture conditions. . Vet Microbiol 18, 177190. [View Article] [PubMed]
    [Google Scholar]
  24. Vartoukian S. R., Palmer R. M., Wade W. G. ( 2007 ). The division “Synergistes”. . Anaerobe 13, 99106. [View Article] [PubMed]
    [Google Scholar]
  25. Vartoukian S. R., Palmer R. M., Wade W. G. ( 2010 ). Cultivation of a Synergistetes strain representing a previously uncultivated lineage. . Environ Microbiol 12, 916928. [View Article] [PubMed]
    [Google Scholar]
  26. Wilmotte A., Van der Auwera G., De Wachter R. ( 1993 ). Structure of the 16 S ribosomal RNA of the thermophilic cyanobacterium Chlorogloeopsis HTF (‘Mastigocladus laminosus HTF‘) strain PCC7518, and phylogenetic analysis. . FEBS Lett 317, 96100. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.044719-0
Loading
/content/journal/ijsem/10.1099/ijs.0.044719-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error