1887

Abstract

A novel anaerobic, mesophilic, amino-acid-fermenting bacterium, designated strain CL-84, was isolated from the swine intestinal tract on mucin-based media. Cells were curved rods (0.8–1.2×3.5–5.0 µm), stained Gram-negative and were non-motile with no evidence of spores. Strain CL-84 produced acetate, propionate, formate and butyrate as the end products of metabolism when grown on serine. Optimum growth occurred at 39 °C and pH 6.5. The major cellular fatty acids were iso-C, iso-C 3-OH, iso-C and C, distinguishing strain CL-84 from closely related species. The DNA G+C content of strain CL-84 was 55.1 mol%. 16S rRNA gene sequence analysis showed that strain CL-84 shared 90–95 % similarity with characterized genera within the phylum , family Phylogenetic analysis showed that strain CL-84 was related to, but distinct from, Based on these findings, we propose that strain CL-84 represents a novel species of the genus . We further propose the name sp. nov. be designated for this species. The type strain is CL-84 ( = DSM 25858 = CCUG 62631). An emended description of the genus is provided.

Funding
This study was supported by the:
  • , Agricultural Research Service
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2013-06-01
2020-07-02
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References

  1. Allen A., Hutton D. A., Pearson J. P. ( 1998 ). The MUC2 gene product: a human intestinal mucin. . Int J Biochem Cell Biol 30, 797801. [CrossRef] [PubMed]
    [Google Scholar]
  2. Allen H. K., Cloud-Hansen K. A., Wolinski J. M., Guan C., Greene S., Lu S., Boeyink M., Broderick N. A., Raffa K. F., Handelsman J. ( 2009 ). Resident microbiota of the gypsy moth midgut harbors antibiotic resistance determinants. . DNA Cell Biol 28, 109117. [CrossRef] [PubMed]
    [Google Scholar]
  3. Allison M. J., Mayberry W. R., McSweeney C. S., Stahl D. A. ( 1992 ). Synergistes jonesii, gen. nov., sp. nov.: a rumen bacterium that degrades toxic pyridinediols. . Syst Appl Microbiol 15, 522529. [CrossRef]
    [Google Scholar]
  4. Bradshaw D. J., Homer K. A., Marsh P. D., Beighton D. ( 1994 ). Metabolic cooperation in oral microbial communities during growth on mucin. . Microbiology 140, 34073412. [CrossRef] [PubMed]
    [Google Scholar]
  5. Buckel W., Dorn U., Semmler R. ( 1981 ). Glutaconate CoA-transferase from Acidaminococcus fermentans . . Eur J Biochem 118, 315321. [CrossRef] [PubMed]
    [Google Scholar]
  6. Cole J. R., Wang Q., Cardenas E., Fish J., Chai B., Farris R. J., Kulam-Syed-Mohideen A. S., McGarrell D. M., Marsh T. et al. ( 2009 ). The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. . Nucleic Acids Res 37 (Database issue), D141D145. [CrossRef] [PubMed]
    [Google Scholar]
  7. Dale J. W., Greenaway P. J. ( 1985 ). Preparation of chromosomal DNA from E. coli . . Methods Mol Biol 2, 197200.[PubMed]
    [Google Scholar]
  8. Downes J., Sutcliffe I., Tanner A. C., Wade W. G. ( 2005 ). Prevotella marshii sp. nov. and Prevotella baroniae sp. nov., isolated from the human oral cavity. . Int J Syst Evol Microbiol 55, 15511555. [CrossRef] [PubMed]
    [Google Scholar]
  9. Downes J., Vartoukian S. R., Dewhirst F. E., Izard J., Chen T., Yu W. H., Sutcliffe I. C., Wade W. G. ( 2009 ). Pyramidobacter piscolens gen. nov., sp. nov., a member of the phylum ‘Synergistetes’ isolated from the human oral cavity. . Int J Syst Evol Microbiol 59, 972980. [CrossRef] [PubMed]
    [Google Scholar]
  10. Finnie I. A., Dwarakanath A. D., Taylor B. A., Rhodes J. M. ( 1995 ). Colonic mucin synthesis is increased by sodium butyrate. . Gut 36, 9399. [CrossRef] [PubMed]
    [Google Scholar]
  11. Ganesan A., Chaussonnerie S., Tarrade A., Dauga C., Bouchez T., Pelletier E., Le Paslier D., Sghir A. ( 2008 ). Cloacibacillus evryensis gen. nov., sp. nov., a novel asaccharolytic, mesophilic, amino-acid-degrading bacterium within the phylum ‘Synergistetes’, isolated from an anaerobic sludge digester. . Int J Syst Evol Microbiol 58, 20032012. [CrossRef] [PubMed]
    [Google Scholar]
  12. Greene R. A., Blum E. F., DeCoro C. T., Fairchild R. B., Kaplan M. T., Landau J. L., Sharp T. R. ( 1951 ). Rapid methods for the detection of motility. . J Bacteriol 62, 347.[PubMed]
    [Google Scholar]
  13. Hatayama H., Iwashita J., Kuwajima A., Abe T. ( 2007 ). The short chain fatty acid, butyrate, stimulates MUC2 mucin production in the human colon cancer cell line, LS174T. . Biochem Biophys Res Commun 356, 599603. [CrossRef] [PubMed]
    [Google Scholar]
  14. Horz H. P., Citron D. M., Warren Y. A., Goldstein E. J., Conrads G. ( 2006 ). Synergistes group organisms of human origin. . J Clin Microbiol 44, 29142920. [CrossRef] [PubMed]
    [Google Scholar]
  15. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. et al. ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [CrossRef] [PubMed]
    [Google Scholar]
  16. Marchandin H., Damay A., Roudière L., Teyssier C., Zorgniotti I., Dechaud H., Jean-Pierre H., Jumas-Bilak E. ( 2010 ). Phylogeny, diversity and host specialization in the phylum Synergistetes with emphasis on strains and clones of human origin. . Res Microbiol 161, 91100. [CrossRef] [PubMed]
    [Google Scholar]
  17. Martens E. C., Chiang H. C., Gordon J. I. ( 2008 ). Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. . Cell Host Microbe 4, 447457. [CrossRef] [PubMed]
    [Google Scholar]
  18. Nataro J. P. ( 2005 ). Colonization of Mucosal Surfaces. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  19. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. ( 2007 ). SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. . Nucleic Acids Res 35, 71887196. [CrossRef] [PubMed]
    [Google Scholar]
  20. Salanitro J. P., Muirhead P. A. ( 1975 ). Quantitative method for the gas chromatographic analysis of short-chain monocarboxylic and dicarboxylic acids in fermentation media. . Appl Microbiol 29, 374381.[PubMed]
    [Google Scholar]
  21. Schrager J. ( 1970 ). The chemical composition and function of gastrointestinal mucus. . Gut 11, 450456. [CrossRef] [PubMed]
    [Google Scholar]
  22. Stackebrandt E., Goodfellow M. ( 1991 ). Nucleic Acid Techniques in Bacterial Systematics. New York:: Wiley;.
    [Google Scholar]
  23. Stanton T. B., Lebo D. F. ( 1988 ). Treponema hyodysenteriae growth under various culture conditions. . Vet Microbiol 18, 177190. [CrossRef] [PubMed]
    [Google Scholar]
  24. Vartoukian S. R., Palmer R. M., Wade W. G. ( 2007 ). The division “Synergistes”. . Anaerobe 13, 99106. [CrossRef] [PubMed]
    [Google Scholar]
  25. Vartoukian S. R., Palmer R. M., Wade W. G. ( 2010 ). Cultivation of a Synergistetes strain representing a previously uncultivated lineage. . Environ Microbiol 12, 916928. [CrossRef] [PubMed]
    [Google Scholar]
  26. Wilmotte A., Van der Auwera G., De Wachter R. ( 1993 ). Structure of the 16 S ribosomal RNA of the thermophilic cyanobacterium Chlorogloeopsis HTF (‘Mastigocladus laminosus HTF‘) strain PCC7518, and phylogenetic analysis. . FEBS Lett 317, 96100. [CrossRef] [PubMed]
    [Google Scholar]
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