RT Journal Article SR Electronic(1) A1 Yamauchi, Yuto A1 Minegishi, Hiroaki A1 Echigo, Akinobu A1 Shimane, Yasuhiro A1 Shimoshige, Hirokazu A1 Kamekura, Masahiro A1 Itoh, Takashi A1 Doukyu, Noriyuki A1 Inoue, Akira A1 Usami, RonYR 2013 T1 Halarchaeum salinum sp. nov., a moderately acidophilic haloarchaeon isolated from commercial sea salt JF International Journal of Systematic and Evolutionary Microbiology, VO 63 IS Pt_3 SP 1138 OP 1142 DO https://doi.org/10.1099/ijs.0.044693-0 PB Microbiology Society, SN 1466-5034, AB Three halophilic archaeal strains, MH1-34-1T, MH1-16-1 and MH1-224-5 were isolated from commercial salt samples produced from seawater in Indonesia, the Philippines and Japan, respectively. Cells of the three strains were pleomorphic and stained Gram-negative. Strain MH1-34-1T was orange–red pigmented, while MH1-16-1 and MH1-224-5 were pink-pigmented. Strain MH1-34-1T was able to grow at 12–30 % (w/v) NaCl (with optimum at 18 % NaCl, w/v) at pH 4.5–7.2 (optimum, pH 5.2–5.5) and at 15–45 °C (optimum, 42 °C). Strains MH1-16-1 and MH1-224-5 grew in slightly different ranges. These strains required at least 1 mM Mg2+ for growth. The 16S rRNA gene sequences of strains MH1-34-1T, MH1-16-1 and MH1-224-5 were almost identical (99.8–99.9 % similarities), and the closest relative was Halarchaeum acidiphilum MH-1-52-1T with 98.4 % similarities. The DNA G+C contents of MH1-34-1T, MH1-16-1 and MH1-224-5 were 59.3, 60.8 and 61.0 mol%, respectively. The level of DNA–DNA relatedness amongst the three strains was 90–91 %, while that between each of the three strains and Halarchaeum acidiphilum MH1-52-1T was 51–55 %. Based on the phenotypic, genotypic and phylogenetic analyses, it is proposed that the isolates should represent a novel species of the genus Halarchaeum , for which the name Halarchaeum salinum sp. nov. is proposed. The type strain is MH1-34-1T ( = JCM 16330T = CECT 7574T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.044693-0