1887

Abstract

Our previous published data indicated that the two rhizobial strains SCAU7 and SCAU27, which were isolated from the root nodules of and respectively, in Sichuan Province, China, might be novel species of the genus . Their exact taxonomic position was determined in the present study by using polyphasic approaches. Comparative analysis of nearly full-length 16S rRNA gene sequences showed that these strains belonged to the genus , with USDA 3383, NZP 2213, CCBAU 65327 and WSM2073 as the closest neighbours (>99 % 16S rRNA gene sequence similarity). Phylogenies of the housekeeping genes and confirmed their distinct position, showing low similarity with respect to those of LMG 6125 (96.5 % and 92.3 % similarity respectively), USDA 3383 (96.8 % and 93.3 % similarity, respectively), CCBAU 65327 (96.5 % and 92.7 % similarity, respectively) and WSM2073 (95.4 % and 90.6 % similarity, respectively). The DNA–DNA relatedness values between strain SCAU7 and strain SCAU27 were 83.0 %, showing that they belong to the same species. The DNA–DNA relatedness values of SCAU7 with NZP 2213, USDA 3383 and CCBAU 65327 were 41.1 %, 48.8 % and 23.4 %, respectively, clearly indicating that strain SCAU7 represents a novel species. A series of phenotypic and genotypic tests and comparison of cellular fatty acids indicated that the novel group of isolates was distinct from previously described species. Therefore, we propose that strains SCAU7 and SCAU27 represent a novel species of the genus sp. nov., with strain SCAU7 ( = HAMBI 3318 = ACCC 13218) as the type strain.

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2013-08-01
2019-10-20
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