1887

Abstract

A novel Gram-negative, rod–coccus shaped, non-motile, strain, RS-3, was isolated from a sediment sample collected from the marine transect of Kongsfjorden, Ny-Ålesund, Svalbard, Arctic. Colonies and broth cultures were yellowish in colour due to the presence of carotenoids. Strain RS-3 was positive for oxidase, aesculinase, caseinase, gelatinase and urease activities and negative for amylase, catalase, lipase, lysine decarboxylase, ornithine decarboxylase, DNase and β-galactosidase activities. The predominant fatty acids were iso-C (18.0), anteiso-C (16.8), iso-C G (14.2), anteiso-C A (6.0) and iso-C 3-OH (6.8). Strain RS-3 contained MK-6 (72.42 %) and MK-7 (27.58 %) as the major respiratory quinones and phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids make up the polar lipid composition. The DNA G+C content of strain RS-3 was 34.7±1.2 mol%. The 16S rRNA gene sequence analysis indicated that and are the most closely related species with sequence similarities to the type strains of these species of 98.5 and 97.7 %, respectively. However, DNA–DNA hybridization with KCTC 22997 and DSM 15363 showed a relatedness of 22 and 42.5 % with respect to strain RS-3. Based on the DNA–DNA hybridization values, phenotypic and chemotaxonomic characteristics and phylogenetic inference, strain RS-3 is proposed as a novel species of the genus , for which the name sp. nov. is proposed. The type strain of sp. nov. is RS-3 ( = CIP 110154 = NBRC 106169). An emended description of the genus is provided.

Funding
This study was supported by the:
  • , Council of Scientific and Industrial Research (CSIR) , (Award NWP0006)
  • , CSIR
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2013-05-01
2021-01-26
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