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Abstract

A rod-shaped, non-motile, Gram-stain-positive, catalase-positive, starch-hydrolysing strain, JC16, was isolated from a semi-arid tropical soil from India. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain JC16 clustered with the type species of the genus , MW 10, a member of the family within the phylum with 99.3 % sequence similarity. Major (>10 %) fatty acids of strain JC16 were iso-C and iso-C. Minor (<10 and >1 %) amounts of C, iso-C, anteiso-C, iso-C, anteiso-C, iso-Cω10 and Cω11 are present in strain JC16. Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipids (PL2–4), aminolipids (AL1, 2) and an unknown lipid. Cell wall peptidoglycan was of the type -Orn–-Glu. The quinone system was composed of MK-7, MK-8 and MK-6. Genomic DNA G+C content of strain JC16 was 57.6 mol%. Distinct physiological, chemotaxonomic and genotypic differences (37 % reassociation based on DNA–DNA hybridization) from MW 10 support the classification of strain JC16 as a representative of a novel species in the genus , for which the name sp. nov. (type strain JC16 = DSM 23442 = NBRC 105215) is proposed.

Funding
This study was supported by the:
  • , JNT University, Hyderabad
  • , Council for Scientific and Industrial Research
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2013-07-01
2020-10-27
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