1887

Abstract

Two bacterial strains, designated JAM1 and JAM7, were isolated from a methanol-fed denitrification system treating seawater at the Montreal Biodome, Canada. They were affiliated within the genus of the Gammaproteobacteria by analysis of the 16S rRNA gene sequences. Strain JAM1 had the capacity to grow under denitrifying conditions by reducing nitrate into nitrite which is unique among the species of the genus . Major fatty acids were Cω7 or ω6, C and Cω7 or ω6. The major ubiquinone was Q. Both strains required vitamin B and Na ions for growth. The genomes of strains JAM1 and JAM7 have been completely sequenced and showed a DNA G+C content of 44.7 mol% and 47.8 mol%, respectively. Growth occurred at pH 6–11 and at 0.5–8 % NaCl. Both genomes contained predicted ORFs encoding the key enzymes of the ribulose monophosphate pathway. Also, operons encoding two nitrate reductases (Nar), two nitric oxide reductases (Nor), one nitrous oxide reductase (Nos) and one truncated nitrite reductase (NirK) were clustered in a 67 kb chromosomal region in strain JAM1. No such operons were found in strain JAM7. These results supported the affiliation of the two strains as novel species within the genus . The names sp. nov. for type strain JAM1 ( = DSM 25689 = ATCC BAA-2433) and sp. nov. for type strain JAM7 ( = DSM 25690 = ATCC BAA-2434) are proposed.

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2013-06-01
2019-12-14
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