%0 Journal Article %A Madhaiyan, Munusamy %A Jin, Tan Yuan %A Roy, Joseph Jegan %A Kim, Soo-Jin %A Weon, Hang-Yeon %A Kwon, Soon-Wo %A Ji, Lianghui %T Pleomorphomonasdiazotrophica sp. nov., an endophytic N-fixing bacterium isolated from root tissue of Jatropha curcas L. %D 2013 %J International Journal of Systematic and Evolutionary Microbiology, %V 63 %N Pt_7 %P 2477-2483 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.044461-0 %I Microbiology Society, %X A novel aerobic, non-motile, pleomorphic, Gram-negative and nitrogen-fixing bacterial strain, designated R5-392T, was isolated from surface-sterilized root tissue of Jatropha curcas. The organism grew optimally at 30 °C in media containing 1 % (w/v) NaCl and at pH 6.0–8.0. The predominant ubiquinone was Q-10 and the major fatty acids were C18 : 1ω7c/C18 : 1ω6c, C16 : 0 and C19 : 0 cyclo ω8c. The DNA G+C content was 63.2 mol%. Analysis of the 16S rRNA gene sequence suggested that strain R5-392T is affiliated with the order Rhizobiales within the class Alphaproteobacteria and is most closely related to Pleomorphomonas oryzae F-7T (98.8 % similarity) and Pleomorphomonas koreensis Y9T (98.3 % similarity). Analysis of partial nifH gene sequences also revealed a monophyletic lineage within the class Alphaproteobacteria , and strain R5-392T was most closely related to P. oryzae F-7T (98 %). Highest nitrogenase activity was detected in the presence of low-level organic nitrogen or in the presence of nitrogenase co-factors (Fe/Mo) in N-free media. Phenotypic and chemotaxonomic data suggest that strain R5-392T represents a novel species within the genus Pleomorphomonas , for which the name Pleomorphomonas diazotrophica sp. nov. is proposed. The type strain is R5-392T ( = KACC 16233T  = DSM 25022T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.044461-0