1887

Abstract

A novel aerobic, non-motile, pleomorphic, Gram-negative and nitrogen-fixing bacterial strain, designated R5-392, was isolated from surface-sterilized root tissue of . The organism grew optimally at 30 °C in media containing 1 % (w/v) NaCl and at pH 6.0–8.0. The predominant ubiquinone was Q-10 and the major fatty acids were Cω7/Cω6, C and C cyclo ω8. The DNA G+C content was 63.2 mol%. Analysis of the 16S rRNA gene sequence suggested that strain R5-392 is affiliated with the order within the class and is most closely related to F-7 (98.8 % similarity) and Y9 (98.3 % similarity). Analysis of partial gene sequences also revealed a monophyletic lineage within the class , and strain R5-392 was most closely related to F-7 (98 %). Highest nitrogenase activity was detected in the presence of low-level organic nitrogen or in the presence of nitrogenase co-factors (Fe/Mo) in N-free media. Phenotypic and chemotaxonomic data suggest that strain R5-392 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is R5-392 ( = KACC 16233  = DSM 25022).

Funding
This study was supported by the:
  • , The Temasek Trust
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2013-07-01
2020-06-01
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