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Abstract
A novel actinomycete, designated strain KLBMP 1279T, was isolated from surface-sterilized roots of a coastal halophyte, Salicornia europaea Linn., collected from Jiangsu Province, in the east of China. The taxonomic status of this organism was established using a polyphasic approach. 16S rRNA gene sequence analysis indicated that strain KLBMP 1279T was closely related to Modestobacter marinus 42H12-1T (99.5 % 16S rRNA gene sequence similarity), Modestobacter versicolor CP153-2T (98.4 %) and Modestobacter multiseptatus AA-826T (97.5 %). Chemotaxonomic characteristics were consistent with its assignment to the genus Modestobacter in that the isolate had meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall, MK-9(H4) as major menaquinone and a polar lipid profile containing diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, two unknown aminophospholipids and an unknown phospholipid. The predominant fatty acids were iso-C16 : 0, iso-C15 : 0 and C17 : 1ω8c. The DNA G+C content was 71.7 mol%. However, DNA–DNA hybridization assays as well as physiological and biochemical analyses differentiated strain KLBMP 1279T from its closest phylogenetic relatives. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, the isolate KLBMP 1279T represents a novel species of the genus Modestobacter , for which the name Modestobacter roseus sp. nov. is proposed; the type strain is KLBMP 1279T ( = KCTC 19887T = NBRC 108673T = DSM 45764T). An emended description of the genus Modestobacter is also proposed.
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Funding
- National Natural Science Foundation of China (Award 31101502 and 31000005)
- Program of Natural Science Foundation of the Jiangsu Higher Education Institutions of China (Award 11KJD210002 and 10KJB180008)
- Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)