1887

Abstract

A novel red-pigmented bacterial strain, designated WS 4628, was isolated from a pharmaceutical clean room of a vaccine-producing company and was investigated in a taxonomic study using a polyphasic approach. The strain was Gram-stain-positive, strictly aerobic, motile, catalase-positive and produced spherical to slightly ellipsoidal endospores in rods. The genomic DNA G+C content was 44.1 mol%. The major fatty acids were anteiso-C, iso-C and anteiso-C and the predominant quinone was MK-6. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid and an unidentified phospholipid. -diaminopimelic acid (type A1γ) was present in the cell-wall peptidoglycan and the major whole-cell sugars were glucose and ribose. The closest phylogenetic neighbours were identified as ATCC 14574 (95.8 % 16S rRNA gene sequence similarity), Sd/3 (94.8 %), YKJ-13 (94.8 %) and JG-30 (94.8 %). Phylogenetic, physiological, biochemical and morphological differences between strain WS 4628 and its closest relatives in the families and suggest that this strain represents a novel species in a new genus in the family for which the name gen. nov., sp. nov. is proposed; the type strain of the type species is WS 4628 ( = DSM 25058 = LMG 26645).

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2013-06-01
2019-10-17
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References

  1. Cerny G.. ( 1976;). Method for distinction of the Gram-negative from Gram-positive bacteria. . Eur J Appl Microbiol 3:, 223–225. [CrossRef]
    [Google Scholar]
  2. Chen Y.-G., Peng D.-J., Chen Q.-H., Zhang Y.-Q., Tang S.-K., Zhang D.-C., Peng Q.-Z., Li W.-J.. ( 2010;). Jeotgalibacillus soli sp. nov., isolated from non-saline forest soil, and emended description of the genus Jeotgalibacillus. . Antonie van Leeuwenhoek 98:, 415–421. [CrossRef][PubMed]
    [Google Scholar]
  3. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W.. ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef][PubMed]
    [Google Scholar]
  4. Cunha S., Tiago I., Paiva G., Nobre F., da Costa M. S., Veríssimo A.. ( 2012;). Jeotgalibacillus soli sp. nov., a Gram-stain-positive bacterium isolated from soil. . Int J Syst Evol Microbiol 62:, 608–612. [CrossRef][PubMed]
    [Google Scholar]
  5. Euzéby, J. P. (2011). List of Prokaryotic Names with Standing in Nomenclature. http://www.bacterio.cict.fr/index.html.
  6. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  7. Krishnamurthi S., Ruckmani A., Pukall R., Chakrabarti T.. ( 2010;). Psychrobacillus gen. nov. and proposal for reclassification of Bacillus insolitus Larkin & Stokes, 1967, B. psychrotolerans Abd-El Rahman et al., 2002 and B. psychrodurans Abd-El Rahman et al., 2002 as Psychrobacillus insolitus comb. nov., Psychrobacillus psychrotolerans comb. nov. and Psychrobacillus psychrodurans comb. nov.. Syst Appl Microbiol 33:, 367–373. [CrossRef][PubMed]
    [Google Scholar]
  8. Kwon S.-W., Kim B.-Y., Song J., Weon H.-Y., Schumann P., Tindall B. J., Stackebrandt E., Fritze D.. ( 2007;). Sporosarcina koreensis sp. nov. and Sporosarcina soli sp. nov., isolated from soil in Korea. . Int J Syst Evol Microbiol 57:, 1694–1698. [CrossRef][PubMed]
    [Google Scholar]
  9. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. et al. ( 2007;). clustal w and clustal_x version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef][PubMed]
    [Google Scholar]
  10. Logan N. A., De Vos P.. ( 2009;). Genus I. Bacillus. . In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 21–128. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  11. Logan N. A., Berge O., Bishop A. H., Busse H.-J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. et al. ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59:, 2114–2121. [CrossRef][PubMed]
    [Google Scholar]
  12. Munoz R., Yarza P., Ludwig W., Euzéby J., Amann R., Schleifer K. H., Glöckner F.-O., Rosselló-Móra R.. ( 2011;). Release LTPs104 of the All-Species Living Tree. . Syst Appl Microbiol 34:, 169–170. [CrossRef][PubMed]
    [Google Scholar]
  13. Rüger H.-J., Fritze D., Spröer C.. ( 2000;). New psychrophilic and psychrotolerant Bacillus marinus strains from tropical and polar deep-sea sediments and emended description of the species. . Int J Syst Evol Microbiol 50:, 1305–1313. [CrossRef][PubMed]
    [Google Scholar]
  14. Ryu E.. ( 1940;). A simple method of differentiation between Gram-positive and Gram-negative organisms without staining. . Kitasato Arch Exp Med 17:, 58–63.
    [Google Scholar]
  15. Schmidt V. S., Mayr R., Wenning M., Glöckner J., Busse H.-J., Scherer S.. ( 2009;). Bavariicoccus seileri gen. nov., sp. nov., isolated from the surface and smear water of German red smear soft cheese. . Int J Syst Evol Microbiol 59:, 2437–2443. [CrossRef][PubMed]
    [Google Scholar]
  16. Schumann P.. ( 2011;). Peptidoglycan structure. . In Taxonomy of Prokaryotes, Methods in Microbiology, vol. 38, pp. 101–129. Edited by Rainey F., Oren A... London:: Academic Press;. [CrossRef]
    [Google Scholar]
  17. Seeley H. W., Vandemark P. J.. ( 1962;). Microbes in Action. A Laboratory Manual of Microbiology. San Francisco:: Freeman;.
    [Google Scholar]
  18. Seiler H., Bleicher A., Busse H.-J., Hüfner J., Scherer S.. ( 2012a;). Psychroflexus halocasei sp. nov., isolated from a microbial consortium on a cheese. . Int J Syst Evol Microbiol 62:, 1850–1856. [CrossRef][PubMed]
    [Google Scholar]
  19. Seiler H., Schmidt V., Wenning M., Scherer S.. ( 2012b;). Bacillus kochii sp. nov., isolated from foods and a pharmaceutical manufacturing site. . Int J Syst Evol Microbiol 62:, 1092–1098. [CrossRef]
    [Google Scholar]
  20. Suresh K., Prabagaran S. R., Sengupta S., Shivaji S.. ( 2004;). Bacillus indicus sp. nov., an arsenic-resistant bacterium isolated from an aquifer in West Bengal, India. . Int J Syst Evol Microbiol 54:, 1369–1375. [CrossRef][PubMed]
    [Google Scholar]
  21. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  22. Tindall B. J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P.. ( 2010;). Notes on the characterization of prokaryote strains for taxonomic purposes. . Int J Syst Evol Microbiol 60:, 249–266. [CrossRef][PubMed]
    [Google Scholar]
  23. Xu P., Li W.-J., Tang S.-K., Zhang Y.-Q., Chen G.-Z., Chen H.-H., Xu L.-H., Jiang C.-L.. ( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55:, 1149–1153. [CrossRef][PubMed]
    [Google Scholar]
  24. Yoon J.-H., Kang S.-S., Lee K.-C., Kho Y. H., Choi S. H., Kang K. H., Park Y.-H.. ( 2001a;). Bacillus jeotgali sp. nov., isolated from jeotgal, Korean traditional fermented seafood. . Int J Syst Evol Microbiol 51:, 1087–1092. [CrossRef][PubMed]
    [Google Scholar]
  25. Yoon J.-H., Kang S.-S., Lee K.-C., Lee E. S., Kho Y. H., Kang K. H., Park Y.-H.. ( 2001b;). Planomicrobium koreense gen. nov., sp. nov., a bacterium isolated from the Korean traditional fermented seafood jeotgal, and transfer of Planococcus okeanokoites (Nakagawa et al. 1996) and Planococcus mcmeekinii (Junge et al. 1998) to the genus Planomicrobium. . Int J Syst Evol Microbiol 51:, 1511–1520.[PubMed]
    [Google Scholar]
  26. Yoon J.-H., Weiss N., Lee K.-C., Lee I.-S., Kang K. H., Park Y.-H.. ( 2001;c). Jeotgalibacillus alimentarius gen. nov., sp. nov., a novel bacterium isolated from jeotgal with l-lysine in the cell wall, and reclassification of Bacillus marinus Rüger 1983 as Marinibacillus marinus gen. nov., comb. nov.. Int J Syst Evol Microbiol 51:, 2087–2093. [CrossRef][PubMed]
    [Google Scholar]
  27. Yoon J.-H., Kim I.-G., Schumann P., Oh T.-K., Park Y.-H.. ( 2004;). Marinibacillus campisalis sp. nov., a moderate halophile isolate from a marine solar saltern in Korea, with emended description of the genus Marinibacillus. . Int J Syst Evol Microbiol 54:, 1317–1321. [CrossRef][PubMed]
    [Google Scholar]
  28. Yoon J.-H., Lee C.-H., Oh T.-K.. ( 2005;). Bacillus cibi sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 55:, 733–736. [CrossRef][PubMed]
    [Google Scholar]
  29. Yoon J.-H., Kang S.-J., Schumann P., Oh T.-K.. ( 2010;). Jeotgalibacillus salarius sp. nov., isolated from a marine saltern, and reclassification of Marinibacillus marinus and Marinibacillus campisalis as Jeotgalibacillus marinus comb. nov. and Jeotgalibacillus campisalis comb. nov., respectively. . Int J Syst Evol Microbiol 60:, 15–20. [CrossRef][PubMed]
    [Google Scholar]
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