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Volume 63, Issue Pt_6

gen. nov., sp. nov., isolated from a pharmaceutical clean room Free

Abstract

A novel red-pigmented bacterial strain, designated WS 4628, was isolated from a pharmaceutical clean room of a vaccine-producing company and was investigated in a taxonomic study using a polyphasic approach. The strain was Gram-stain-positive, strictly aerobic, motile, catalase-positive and produced spherical to slightly ellipsoidal endospores in rods. The genomic DNA G+C content was 44.1 mol%. The major fatty acids were anteiso-C, iso-C and anteiso-C and the predominant quinone was MK-6. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid and an unidentified phospholipid. -diaminopimelic acid (type A1γ) was present in the cell-wall peptidoglycan and the major whole-cell sugars were glucose and ribose. The closest phylogenetic neighbours were identified as ATCC 14574 (95.8 % 16S rRNA gene sequence similarity), Sd/3 (94.8 %), YKJ-13 (94.8 %) and JG-30 (94.8 %). Phylogenetic, physiological, biochemical and morphological differences between strain WS 4628 and its closest relatives in the families and suggest that this strain represents a novel species in a new genus in the family for which the name gen. nov., sp. nov. is proposed; the type strain of the type species is WS 4628 ( = DSM 25058 = LMG 26645).

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2013-06-01
2024-04-25
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Domibacillus robiginosus gen. nov., sp. nov., isolated from a pharmaceutical clean room
Int J Syst Evol Microbiol 63, 2054 (2013); https://doi.org/10.1099/ijs.0.044396-0
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