1887

Abstract

The bacterial symbiont AM7, isolated from a novel entomopathogenic nematode species of the genus , displays the main phenotypic traits of the genus and is highly pathogenic to . Phylogenetic analysis based on a multigene approach (16S rRNA, , , , and ) confirmed the classification of isolate AM7 within the species and revealed its close relatedness to , and The five concatenated protein-encoding sequences (4197 nt) of strain AM7 revealed 95.8, 95.4 and 94.9 % nucleotide identity to sequences of HG29, FRG04 and C8404, respectively. These identity values are less than the threshold of 97 % proposed for classification within one of the existing subspecies of Unlike other strains described for , strain AM7 produces acid from adonitol, sorbitol and xylitol, assimilates xylitol and has no lipase activity on medium containing Tween 20 or 60. Strain AM7 is differentiated from by the assimilation of -acetylglucosamine and the absence of haemolytic activity. Unlike , strain AM7 does not assimilate mannitol, and it is distinguished from by the assimilation of trehalose and citrate, the inability to produce indole from tryptophan and the presence of acetoin production and urease activity. Strain AM7 ( = ATCC BAA-2407  = DSM 25462) belongs to a novel subspecies, and is proposed as the type strain of subsp. sp. nov.

Funding
This study was supported by the:
  • South African Apple and Pear Producers Association (SAAPPA)
  • Citrus Research International (CRI)
  • Technology and Human Resources for Industry Programme (THRIP)
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2013-05-01
2024-11-13
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