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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 63, Issue Pt_5

sp. nov., isolated from seashore sediment, and emended description of the genus Free

Abstract

A Gram-negative, non-spore-forming, non-flagellated, coccoid-, oval- or rod-shaped strain, designated M-M23, was isolated from seashore sediment at Geoje island, South Korea. Strain M-M23 grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain M-M23 clustered with the type strains of the two species of the genus , with which it exhibited 97.6–98.1 % 16S rRNA gene sequence similarity. Sequence similarity with the type strains of other recognized species was <90.8 %. Strain M-M23 contained Q-10 as the predominant ubiquinone and Cω7 and C as the major fatty acids. The major polar lipids of strain M-M23 were phosphatidylglycerol and two unidentified lipids. The DNA G+C content of strain M-M23 was 45.4 mol%. DNA–DNA relatedness between the isolate and DSM 5838 and JCM 14974 was 22±7.2 and 14±5.6 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain M-M23 is separate from the other described members of the genus . On the basis of the data presented, strain M-M23 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M-M23 ( = KCTC 32081  = CCUG 62793). An emended description of the genus is also provided.

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Funding
This study was supported by the:
  • Program for Collection, Management and Utilization of Biological Resources
  • BK21 program
© 2013 IUMS
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2013-05-01
2025-03-18
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References

  1. Baumann P., Baumann L. ( 1981 ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . . In The Prokaryotes, pp. 1302–1331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. . Berlin:: Springer;.
     [Google Scholar]
  2. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 1997–2006. [View Article] [PubMed]
    [Google Scholar]
  3. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. ( 1957 ). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. . J Cell Comp Physiol 49, 25–68. [View Article]
    [Google Scholar]
  4. Cowan S. T., Steel K. J. ( 1965 ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
     [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224–229. [View Article]
    [Google Scholar]
  6. Kang H. S., Lee S. D. ( 2009 ). Hirschia maritima sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 59, 2264–2268. [View Article] [PubMed]
    [Google Scholar]
  7. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  8. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 1–67. [View Article]
    [Google Scholar]
  9. Lee K.-B., Liu C.-T., Anzai Y., Kim H., Aono T., Oyaizu H. ( 2005 ). The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov.. Int J Syst Evol Microbiol 55, 1907–1919. [View Article] [PubMed]
    [Google Scholar]
  10. Lee K., Lee H. K., Choi T.-H., Cho J.-C. ( 2007 ). Robiginitomaculum antarcticum gen. nov., sp. nov., a member of the family Hyphomonadaceae, from Antarctic seawater. . Int J Syst Evol Microbiol 57, 2595–2599. [View Article] [PubMed]
    [Google Scholar]
  11. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. ( 1977 ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27, 104–117. [View Article]
    [Google Scholar]
  12. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  13. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids. Newark, DE:: MIDI Inc;.
     [Google Scholar]
  14. Schlesner H., Bartels C., Sittig M., Dorsch M., Stackebrandt E. ( 1990 ). Taxonomic and phylogenetic studies on a new taxon of budding, hyphal Proteobacteria, Hirschia baltica gen. nov., sp. nov.. Int J Syst Bacteriol 40, 443–451. [View Article] [PubMed]
    [Google Scholar]
  15. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846–849. [View Article]
    [Google Scholar]
  16. Staley J. T. ( 1968 ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95, 1921–1942.[PubMed]
    [Google Scholar]
  17. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125–128. [View Article]
    [Google Scholar]
  18. Teather R. M., Wood P. J. ( 1982 ). Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. . Appl Environ Microbiol 43, 777–780.[PubMed]
    [Google Scholar]
  19. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 4876–4882. [View Article] [PubMed]
    [Google Scholar]
  20. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  21. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. ( 1996 ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46, 502–505. [View Article]
    [Google Scholar]
  22. Yoon J.-H., Lee S. T., Park Y.-H. ( 1998 ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48, 187–194. [View Article] [PubMed]
    [Google Scholar]
  23. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. ( 2003 ). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. . Int J Syst Evol Microbiol 53, 53–57. [View Article] [PubMed]
    [Google Scholar]
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Hirschia litorea sp. nov., isolated from seashore sediment, and emended description of the genus Hirschia
Int J Syst Evol Microbiol 63, 1684 (2013); https://doi.org/10.1099/ijs.0.044297-0
/content/journal/ijsem/10.1099/ijs.0.044297-0
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