1887

Abstract

An orange-coloured and slender rod-shaped bacterium, designated strain PSC32, was isolated from the gut microflora of a mussel collected from Gwangyang Bay, South Sea (Republic of Korea). Cells were Gram-reaction-negative, strictly aerobic, and catalase- and oxidase-positive. The major fatty acids were iso-C, iso-C 3-OH, iso-C G and iso-C 3-OH. The only isoprenoid quinone of strain PSC32 was MK-6 and the DNA G+C content was 36.9 mol%. Phosphatidylethanolamine, one unidentified aminolipid and two unidentified polar lipids were found as major polar lipids. A phylogenetic tree based on 16S rRNA gene sequences showed that strain PSC32 forms an evolutionary lineage within the genus and is closely related to A6 (97.0 % similarity) and to other members of the genus (94.4–96.5 %). Genomic DNA–DNA relatedness between strain PSC32 and A6 was 40.7 %. A number of phenotypic characteristics distinguished strain PSC32 from described members of the genus . On the basis of the evidence presented in this study, strain PSC32 represents a novel species, for which the name sp. nov. is proposed. The type strain is PSC32 ( = KCTC 23301 = JCM 17453). An emended description of is given.

Funding
This study was supported by the:
  • National Institute of Biological Resources (NIBR)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.044289-0
2013-05-01
2021-10-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/5/1782.html?itemId=/content/journal/ijsem/10.1099/ijs.0.044289-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A. (editors) ( 1993 ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [View Article] [PubMed]
    [Google Scholar]
  3. Chen W. M., Sheu F. S., Sheu S. Y. ( 2012 ). Aquimarina salinaria sp. nov., a novel algicidal bacterium isolated from a saltpan. . Arch Microbiol 194, 103112. [View Article] [PubMed]
    [Google Scholar]
  4. Chun J., Goodfellow M. ( 1995 ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. . Int J Syst Bacteriol 45, 240245. [View Article] [PubMed]
    [Google Scholar]
  5. CLSI ( 2009 ). Performance Standards for Antimicrobial Susceptibility Testing; Approved Standard, 19th Informational Supplement M100–S19 (ISBN 1-56238-690-5). . Wayne, PA:: Clinical and Laboratory Standards Institute;.
  6. Collins M. D. ( 1994 ). Isoprenoid quinones. . In Chemical Methods in Prokaryotic Systematics, pp. 265309. Edited by Goodfellow M., O’Donnell A. G. . Chichester:: Wiley;.
    [Google Scholar]
  7. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  8. Felsenstein J. ( 1993 ). phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  10. Fitch W. M., Margoliash E. ( 1967 ). Construction of phylogenetic trees. . Science 155, 279284. [View Article] [PubMed]
    [Google Scholar]
  11. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21132. Edited by Munro H. N. . New York:: Academic Press;.
    [Google Scholar]
  12. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  13. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  14. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [View Article]
    [Google Scholar]
  15. Lee J.-S., Lee K. C., Pyun Y.-R., Bae K. S. ( 2003 ). Arthrobacter koreensis sp. nov., a novel alkalitolerant bacterium from soil. . Int J Syst Evol Microbiol 53, 12771280. [View Article] [PubMed]
    [Google Scholar]
  16. Lin B., Lu G., Zheng Y., Xie W., Li S., Hu Z. ( 2012 ). Aquimarina agarilytica sp. nov., an agarolytic species isolated from a red alga. . Int J Syst Evol Microbiol 62, 869873. [View Article] [PubMed]
    [Google Scholar]
  17. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109118. [View Article] [PubMed]
    [Google Scholar]
  18. McCammon S. A., Bowman J. P. ( 2000 ). Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov., and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov.. Int J Syst Evol Microbiol 50, 10551063. [View Article] [PubMed]
    [Google Scholar]
  19. MIDI ( 2001 ). Sherlock Microbial Identification System Operating Manual, version 4.0. Newark, DE: MIDI, Inc.
  20. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  21. Miyazaki M., Nagano Y., Fujiwara Y., Hatada Y., Nogi Y. ( 2010 ). Aquimarina macrocephali sp. nov., isolated from sediment adjacent to sperm whale carcasses. . Int J Syst Evol Microbiol 60, 22982302. [View Article] [PubMed]
    [Google Scholar]
  22. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Frolova G. M., Mikhailov V. V., Lee K. H., Bae K. S. ( 2005 ). Description of Aquimarina muelleri gen. nov., sp. nov., and proposal of the reclassification of [Cytophaga] latercula Lewin 1969 as Stanierella latercula gen. nov., comb. nov.. Int J Syst Evol Microbiol 55, 225229. [View Article] [PubMed]
    [Google Scholar]
  23. Nedashkovskaya O. I., Vancanneyt M., Christiaens L., Kalinovskaya N. I., Mikhailov V. V., Swings J. ( 2006 ). Aquimarina intermedia sp. nov., reclassification of Stanierella latercula (Lewin 1969) as Aquimarina latercula comb. nov. and Gaetbulimicrobium brevivitae Yoon et al. 2006 as Aquimarina brevivitae comb. nov. and emended description of the genus Aquimarina . . Int J Syst Evol Microbiol 56, 20372041. [View Article] [PubMed]
    [Google Scholar]
  24. Nedashkovskaya O. I., Vancanneyt M., Kim S. B. ( 2011 ). Genus IV. Aquimarina . . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 158161. Edited by Krieg N. R., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J. , Staley J. T., Ward N., Brown D., Parte A. . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  25. Park S. C., Choe H. N., Baik K. S., Seong C. N. ( 2012 ). Aquimarina mytili sp. nov., isolated from the gut microflora of a mussel, Mytilus coruscus, and emended description of Aquimarina macrocephali . . Int J Syst Evol Microbiol 62, 19741979. [View Article] [PubMed]
    [Google Scholar]
  26. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  27. Smibert R. M., Krieg N. R. ( 1994 ). General characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  28. Swofford D. L. ( 1998 ). Phylogenetic analysis using parsimony (paup). Version 4. Sunderland, MA:: Sinauer Associates;.
    [Google Scholar]
  29. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  31. Yang S.-H., Kwon K. K., Lee H.-S., Kim S.-J. ( 2006 ). Shewanella spongiae sp. nov., isolated from a marine sponge. . Int J Syst Evol Microbiol 56, 28792882. [View Article] [PubMed]
    [Google Scholar]
  32. Yi H., Chun J. ( 2011 ). Aquimarina addita sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 61, 24452449. [View Article] [PubMed]
    [Google Scholar]
  33. Yoon B. J., You H. S., Lee D. H., Oh D. C. ( 2011 ). Aquimarina spongiae sp. nov., isolated from marine sponge Halichondria oshoro . . Int J Syst Evol Microbiol 61, 417421. [View Article] [PubMed]
    [Google Scholar]
  34. Yumoto I., Hirota K., Yamaga S., Nodasaka Y., Kawasaki T., Matsuyama H., Nakajima K. ( 2004 ). Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids. . Int J Syst Evol Microbiol 54, 19972001. [View Article] [PubMed]
    [Google Scholar]
  35. ZoBell C. E. ( 1941 ). Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. . J Mar Res 4, 4275.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.044289-0
Loading
/content/journal/ijsem/10.1099/ijs.0.044289-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error