1887

Abstract

A novel yeast species is described based on three strains from the gut of wood-boring larvae collected in a tree trunk of cultivated in parks near Nanyang, central China. Phylogenetic analysis based on sequences of the D1/D2 domains of the large subunit rRNA gene showed that these strains occurred in a separate clade that was genetically distinct from all known ascomycetous yeasts. In terms of pairwise sequence divergence, the novel strains differed by 15.3 % divergence from the type strain of , and by 15.8 % divergence from the type strains of and in the D1/D2 domains. All three are ascomycetous yeasts in the clade. Unlike . , . and . , the novel isolates did not ferment glucose. The name sp. nov. is proposed to accommodate these highly divergent organisms, with STN-8 ( = CICC 1980 = CBS 12638) as the type strain.

Funding
This study was supported by the:
  • Research Planning Project of Basic and Advanced Technology of Henan Province, China (Award 122300410032)
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2012-11-01
2021-10-23
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References

  1. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  2. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  3. Kurtzman C. P., Fell J. W. 2006; Yeast systematics and phylogeny: implications of molecular identification methods for studies in ecology. In Biodiversity and Ecophysiology of Yeasts pp. 11–30 Edited by Rosa C., Péter G. Berlin: Springer; [View Article]
    [Google Scholar]
  4. Kurtzman C. P., Robnett C. J. 1998; Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73:331–371 [View Article][PubMed]
    [Google Scholar]
  5. Lachance M.-A., Boekhout T., Scorzetti G., Fell J. W., Kurtzman C. P. 2011; Candida Berkhout. In The Yeasts, a Taxonomic Study, 5th edn. pp. 987–1278 Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [View Article]
    [Google Scholar]
  6. Middelhoven W. J., Kurtzman C. P. 2003; Relation between phylogeny and physiology in some ascomycetous yeasts. Antonie van Leeuwenhoek 83:69–74 [View Article][PubMed]
    [Google Scholar]
  7. Nguyen N. H., Suh S. O., Erbil C. K., Blackwell M. 2006; Metschnikowia noctiluminum sp. nov., Metschnikowia corniflorae sp. nov., and Candida chrysomelidarum sp. nov., isolated from green lacewings and beetles. Mycol Res 110:346–356 [View Article][PubMed]
    [Google Scholar]
  8. Ramos J. P., Valente P., de Souza R. A., Rosa C. A., Leoncini O. 2001; Heteroduplex mobility assay of the D1/D2 region of the 26S rDNA for differentiation of Saccharomyces species. Lett Appl Microbiol 33:206–210 [View Article][PubMed]
    [Google Scholar]
  9. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  10. Scorzetti G., Fell J. W., Fonseca A., Statzell-Tallman A. 2002; Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res 2:495–517[PubMed] [CrossRef]
    [Google Scholar]
  11. Suh S. O., McHugh J. V., Blackwell M. 2004; Expansion of the Candida tanzawaensis yeast clade: 16 novel Candida species from basidiocarp-feeding beetles. Int J Syst Evol Microbiol 54:2409–2429 [View Article][PubMed]
    [Google Scholar]
  12. Thanh V. N., Hai D. A., Lachance M.-A. 2003; Issatchenkia hanoiensis, a new yeast species isolated from frass of the litchi fruit borer Conopomorpha cramerella Snellen. FEMS Yeast Res 4:113–117 [View Article][PubMed]
    [Google Scholar]
  13. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  14. Yamada Y., Kondo K. 1973; Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus and the yeast-like genera Sporobolomyces and Rhodosporidium . J Gen Appl Microbiol 19:59–77 [View Article]
    [Google Scholar]
  15. Yarrow D. 1998; Methods for the isolation and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn. pp. 77–100 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier; [View Article]
    [Google Scholar]
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