1887

Abstract

A novel yeast species is described based on three strains from the gut of wood-boring larvae collected in a tree trunk of cultivated in parks near Nanyang, central China. Phylogenetic analysis based on sequences of the D1/D2 domains of the large subunit rRNA gene showed that these strains occurred in a separate clade that was genetically distinct from all known ascomycetous yeasts. In terms of pairwise sequence divergence, the novel strains differed by 15.3 % divergence from the type strain of , and by 15.8 % divergence from the type strains of and in the D1/D2 domains. All three are ascomycetous yeasts in the clade. Unlike . , . and . , the novel isolates did not ferment glucose. The name sp. nov. is proposed to accommodate these highly divergent organisms, with STN-8 ( = CICC 1980 = CBS 12638) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.044214-0
2012-11-01
2020-01-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/11/2805.html?itemId=/content/journal/ijsem/10.1099/ijs.0.044214-0&mimeType=html&fmt=ahah

References

  1. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  2. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  3. Kurtzman C. P. , Fell J. W. . ( 2006; ). Yeast systematics and phylogeny: implications of molecular identification methods for studies in ecology. . In Biodiversity and Ecophysiology of Yeasts, pp. 11–30. Edited by Rosa C. , Péter G. . . Berlin:: Springer;. [CrossRef]
    [Google Scholar]
  4. Kurtzman C. P. , Robnett C. J. . ( 1998; ). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. . Antonie van Leeuwenhoek 73:, 331–371. [CrossRef] [PubMed]
    [Google Scholar]
  5. Lachance M.-A. , Boekhout T. , Scorzetti G. , Fell J. W. , Kurtzman C. P. . ( 2011; ). Candida Berkhout. . In The Yeasts, a Taxonomic Study, , 5th edn., pp. 987–1278. Edited by Kurtzman C. P. , Fell J. W. , Boekhout T. . . Amsterdam:: Elsevier;. [CrossRef]
    [Google Scholar]
  6. Middelhoven W. J. , Kurtzman C. P. . ( 2003; ). Relation between phylogeny and physiology in some ascomycetous yeasts. . Antonie van Leeuwenhoek 83:, 69–74. [CrossRef] [PubMed]
    [Google Scholar]
  7. Nguyen N. H. , Suh S. O. , Erbil C. K. , Blackwell M. . ( 2006; ). Metschnikowia noctiluminum sp. nov., Metschnikowia corniflorae sp. nov., and Candida chrysomelidarum sp. nov., isolated from green lacewings and beetles. . Mycol Res 110:, 346–356. [CrossRef] [PubMed]
    [Google Scholar]
  8. Ramos J. P. , Valente P. , de Souza R. A. , Rosa C. A. , Leoncini O. . ( 2001; ). Heteroduplex mobility assay of the D1/D2 region of the 26S rDNA for differentiation of Saccharomyces species. . Lett Appl Microbiol 33:, 206–210. [CrossRef] [PubMed]
    [Google Scholar]
  9. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  10. Scorzetti G. , Fell J. W. , Fonseca A. , Statzell-Tallman A. . ( 2002; ). Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. . FEMS Yeast Res 2:, 495–517.[PubMed] [CrossRef]
    [Google Scholar]
  11. Suh S. O. , McHugh J. V. , Blackwell M. . ( 2004; ). Expansion of the Candida tanzawaensis yeast clade: 16 novel Candida species from basidiocarp-feeding beetles. . Int J Syst Evol Microbiol 54:, 2409–2429. [CrossRef] [PubMed]
    [Google Scholar]
  12. Thanh V. N. , Hai D. A. , Lachance M.-A. . ( 2003; ). Issatchenkia hanoiensis, a new yeast species isolated from frass of the litchi fruit borer Conopomorpha cramerella Snellen. . FEMS Yeast Res 4:, 113–117. [CrossRef] [PubMed]
    [Google Scholar]
  13. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  14. Yamada Y. , Kondo K. . ( 1973; ). Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus and the yeast-like genera Sporobolomyces and Rhodosporidium . . J Gen Appl Microbiol 19:, 59–77. [CrossRef]
    [Google Scholar]
  15. Yarrow D. . ( 1998; ). Methods for the isolation and identification of yeasts. . In The Yeasts, a Taxonomic Study, , 4th edn., pp. 77–100. Edited by Kurtzman C. P. , Fell J. W. . . Amsterdam:: Elsevier;. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.044214-0
Loading
/content/journal/ijsem/10.1099/ijs.0.044214-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error