1887

Abstract

A facultatively anaerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-reaction-negative, coccoid to short rod-shaped strain, designated FLN-7, was isolated from activated sludge of a wastewater biotreatment facility. The strain was able to hydrolyse amide pesticides (e.g. diflubenzuron, propanil, chlorpropham and dimethoate) through amide bond cleavage. Strain FLN-7 grew at 4–42 °C (optimum 28 °C), at pH 5.0–8.0 (optimum pH 7.0) and with 0–5.0 % (w/v) NaCl (optimum 1.0 %). The major respiratory quinone was ubiquinone-10. The major cellular fatty acid was Cω7. The genomic DNA G+C content of strain FLN-7 was 66.4±0.5 mol%. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine and an unidentified glycolipid. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain FLN-7 was a member of the genus and showed highest 16S rRNA gene sequence similarities with JCM 7685 (99.2 %), DSM 413 (97.8 %), CDC G1212 (97.3 %) and THI 011 (97.1 %). Strain FLN-7 showed low DNA–DNA relatedness with KACC 1226136.5±3.4 %), KACC 12251 (30.5±2.6 %), CCUG 46822 (26.2±2.4 %) and KACC 13901 (15.5±0.9 %). Based on the phylogenetic analysis, DNA–DNA hybridization, whole-cell fatty acid composition and biochemical characteristics, strain FLN-7 was clearly distinguished from all recognized species of the genus and should be classified in a novel species, for which the name sp. nov. is proposed. The type strain is FLN-7 ( = KACC 16242  = ACCC 05690).

Funding
This study was supported by the:
  • Fundamental Research Funds for the Central Universities (Award KYZ201122)
  • Natural Science Foundation of Jiangsu Province, China (Award BK2011066)
  • National Science and Technology Support Plan Project (Award 2012BAD15B03)
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2013-03-01
2024-12-07
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References

  1. Allen M. B. ( 1959 ). Studies with Cyanidium caldarium, an anomalously pigmented chlorophyte. . Arch Mikrobiol 32, 270277. [View Article] [PubMed]
    [Google Scholar]
  2. An D.-S., Lee H.-G., Lee S.-T., Im W.-T. ( 2009 ). Rhodanobacter ginsenosidimutans sp. nov., isolated from soil of a ginseng field in South Korea. . Int J Syst Evol Microbiol 59, 691694. [View Article] [PubMed]
    [Google Scholar]
  3. Berry A., Janssens D., Hümbelin M., Jore J. P. M., Hoste B., Cleenwerck I., Vancanneyt M., Bretzel W., Mayer A. F. & other authors ( 2003 ). Paracoccus zeaxanthinifaciens sp. nov., a zeaxanthin-producing bacterium. . Int J Syst Evol Microbiol 53, 231238. [View Article] [PubMed]
    [Google Scholar]
  4. Buck J. D. ( 1982 ). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. . Appl Environ Microbiol 44, 992993.[PubMed]
    [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221230. [View Article] [PubMed]
    [Google Scholar]
  6. Cowan S. T., Steel K. J. ( 1965 ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  7. Davis D. H., Doudoroff M., Stanier R. Y., Mandel M. ( 1969 ). Proposal to reject the genus Hydrogenomonas: taxonomic implications. . Int J Syst Bacteriol 19, 375390. [View Article]
    [Google Scholar]
  8. Deng Z.-S., Zhao L.-F., Xu L., Kong Z.-Y., Zhao P., Qin W., Chang J.-L., Wei G.-H. ( 2011 ). Paracoccus sphaerophysae sp. nov., a siderophore-producing, endophytic bacterium isolated from root nodules of Sphaerophysa salsula . . Int J Syst Evol Microbiol 61, 665669. [View Article] [PubMed]
    [Google Scholar]
  9. Denis H., Stephen C. ( 2004 ). Pesticide Residues in Food and Drinking Water: Human Exposure and Risks. New York:: Wiley;.
    [Google Scholar]
  10. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  11. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  12. Frank D. H., Peter G., Nico V. S. (editors) ( 2003 ). Pesticides: Problems, Improvements, Alternatives. NY:: Wiley;.
    [Google Scholar]
  13. Katayama Y., Hiraishi A., Kuraishi H. ( 1995 ). Paracoccus thiocyanatus sp. nov., a new species of thiocyanate-utilizing facultative chemolithotroph, and transfer of Thiobacillus versutus to the genus Paracoccus as Paracoccus versutus comb. nov. with emendation of the genus. . Microbiology 141, 14691477. [View Article] [PubMed]
    [Google Scholar]
  14. Kim B.-Y., Weon H.-Y., Yoo S.-H., Kwon S.-W., Cho Y.-H., Stackebrandt E., Go S.-J. ( 2006 ). Paracoccus homiensis sp. nov., isolated from a sea-sand sample. . Int J Syst Evol Microbiol 56, 23872390. [View Article] [PubMed]
    [Google Scholar]
  15. Kim Y.-O., Kong H. J., Park S., Kang S.-J., Kim K.-K., Moon D. Y., Oh T.-K., Yoon J.-H. ( 2010 ). Paracoccus fistulariae sp. nov., a lipolytic bacterium isolated from bluespotted cornetfish, Fistularia commersonii . . Int J Syst Evol Microbiol 60, 29082912. [View Article] [PubMed]
    [Google Scholar]
  16. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  17. La H.-J., Im W.-T., Ten L. N., Kang M. S., Shin D.-Y., Lee S.-T. ( 2005 ). Paracoccus koreensis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket (UASB) reactor. . Int J Syst Evol Microbiol 55, 16571660. [View Article] [PubMed]
    [Google Scholar]
  18. Lee J. H., Kim Y. S., Choi T.-J., Lee W. J., Kim Y. T. ( 2004 ). Paracoccus haeundaensis sp. nov., a Gram-negative, halophilic, astaxanthin-producing bacterium. . Int J Syst Evol Microbiol 54, 16991702. [View Article] [PubMed]
    [Google Scholar]
  19. Lipski A., Reichert K., Reuter B., Spröer C., Altendorf K. ( 1998 ). Identification of bacterial isolates from biofilters as Paracoccus alkenifer sp. nov. and Paracoccus solventivorans with emended description of Paracoccus solventivorans . . Int J Syst Bacteriol 48, 529536. [View Article] [PubMed]
    [Google Scholar]
  20. Liu X.-Y., Wang B.-J., Jiang C.-Y., Liu S.-J. ( 2006 ). Paracoccus sulfuroxidans sp. nov., a sulfur oxidizer from activated sludge. . Int J Syst Evol Microbiol 56, 26932695. [View Article] [PubMed]
    [Google Scholar]
  21. Liu Z.-P., Wang B.-J., Liu X.-Y., Dai X., Liu Y.-H., Liu S.-J. ( 2008 ). Paracoccus halophilus sp. nov., isolated from marine sediment of the South China Sea, China, and emended description of genus Paracoccus Davis 1969. . Int J Syst Evol Microbiol 58, 257261. [View Article] [PubMed]
    [Google Scholar]
  22. Ludwig W., Mittenhuber G., Friedrich C. G. ( 1993 ). Transfer of Thiosphaera pantotropha to Paracoccus denitrificans . . Int J Syst Bacteriol 43, 363367. [View Article] [PubMed]
    [Google Scholar]
  23. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109118. [View Article] [PubMed]
    [Google Scholar]
  24. Minnikin D. E., Collins M. D., Goodfellow M. ( 1979 ). Fatty-acid and polar lipid-composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Microbiol 47, 8795. [View Article]
    [Google Scholar]
  25. Ohta H., Hattori T. ( 1983 ). Agromonas oligotrophica gen. nov., sp. nov., a nitrogen-fixing oligotrophic bacterium. . Antonie van Leeuwenhoek 49, 429446.[PubMed]
    [Google Scholar]
  26. Pukall R., Laroche M., Kroppenstedt R. M., Schumann P., Stackebrandt E., Ulber R. ( 2003 ). Paracoccus seriniphilus sp. nov., an l-serine-dehydratase-producing coccus isolated from the marine bryozoan Bugula plumosa . . Int J Syst Evol Microbiol 53, 443447. [View Article] [PubMed]
    [Google Scholar]
  27. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  28. Sambrook J., Russell D. W. ( 2001 ). Molecular Cloning: a Laboratory Manual,, 3rd Edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  29. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  30. Siller H., Rainey F. A., Stackebrandt E., Winter J. ( 1996 ). Isolation and characterization of a new gram-negative, acetone-degrading, nitrate-reducing bacterium from soil, Paracoccus solventivorans sp. nov.. Int J Syst Bacteriol 46, 11251130. [View Article] [PubMed]
    [Google Scholar]
  31. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. ( 2001 ). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. Int J Syst Evol Microbiol 51, 16391652. [View Article] [PubMed]
    [Google Scholar]
  32. Tamaoka J., Katayama-Fujimura Y., Kuraishi H. ( 1983 ). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. . J Appl Bacteriol 54, 3136. [View Article]
    [Google Scholar]
  33. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  34. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  35. Tomlin C. D. S. (editor) ( 2007 ). The Pesticide Manual, , 14th edn.. UK:: British Crop Protection Council;.
    [Google Scholar]
  36. Tsubokura A., Yoneda H., Mizuta H. ( 1999 ). Paracoccus carotinifaciens sp. nov., a new aerobic gram-negative astaxanthin-producing bacterium. . Int J Syst Bacteriol 49, 277282. [View Article] [PubMed]
    [Google Scholar]
  37. Urakami T., Araki H., Oyanagi H., Suzuki K., Komagata K. ( 1990 ). Paracoccus aminophilus sp. nov. and Paracoccus aminovorans sp. nov., which utilize N,N-dimethylformamide. . Int J Syst Bacteriol 40, 287291. [View Article] [PubMed]
    [Google Scholar]
  38. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  39. Zhang J., Yin J.-G., Hang B.-J., Cai S., He J., Zhou S.-G., Li S.-P. ( 2012 ). Cloning of a novel arylamidase gene from Paracoccus sp. strain FLN-7 that hydrolyzes amide pesticides. . Appl Environ Microbiol 78, 48484855. [View Article] [PubMed]
    [Google Scholar]
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