1887

Abstract

A facultatively anaerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-reaction-negative, coccoid to short rod-shaped strain, designated FLN-7, was isolated from activated sludge of a wastewater biotreatment facility. The strain was able to hydrolyse amide pesticides (e.g. diflubenzuron, propanil, chlorpropham and dimethoate) through amide bond cleavage. Strain FLN-7 grew at 4–42 °C (optimum 28 °C), at pH 5.0–8.0 (optimum pH 7.0) and with 0–5.0 % (w/v) NaCl (optimum 1.0 %). The major respiratory quinone was ubiquinone-10. The major cellular fatty acid was Cω7. The genomic DNA G+C content of strain FLN-7 was 66.4±0.5 mol%. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine and an unidentified glycolipid. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain FLN-7 was a member of the genus and showed highest 16S rRNA gene sequence similarities with JCM 7685 (99.2 %), DSM 413 (97.8 %), CDC G1212 (97.3 %) and THI 011 (97.1 %). Strain FLN-7 showed low DNA–DNA relatedness with KACC 1226136.5±3.4 %), KACC 12251 (30.5±2.6 %), CCUG 46822 (26.2±2.4 %) and KACC 13901 (15.5±0.9 %). Based on the phylogenetic analysis, DNA–DNA hybridization, whole-cell fatty acid composition and biochemical characteristics, strain FLN-7 was clearly distinguished from all recognized species of the genus and should be classified in a novel species, for which the name sp. nov. is proposed. The type strain is FLN-7 ( = KACC 16242  = ACCC 05690).

Funding
This study was supported by the:
  • , Fundamental Research Funds for the Central Universities , (Award KYZ201122)
  • , Natural Science Foundation of Jiangsu Province, China , (Award BK2011066)
  • , National Science and Technology Support Plan Project , (Award 2012BAD15B03)
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2013-03-01
2020-07-10
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