1887

Abstract

A thermophilic, aerobic, Gram-stain-negative, filamentous bacterium, strain PRI-4131, was isolated from an intertidal hot spring in Isafjardardjup, NW Iceland. The strain grew chemo-organotrophically on various carbohydrates. The temperature range for growth was 40–65 °C (optimum 55 °C), the pH range was pH 6.5–9.0 (optimum pH 7.0) and the NaCl range was 0–3 % (w/v) (optimum 0.5 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PRI-4131 represented a distinct lineage within the class of the phylum . The highest levels of sequence similarity, about 91 %, were with STL-6-O1 and D1-25-10-4. Fermentative growth was not observed for strain PRI-4131, which, in addition to other characteristics, distinguished it from the two species. Owing to both phylogenetic and phenotypic differences from the described members of the class , we propose to accommodate strain PRI-4131 in a novel species in a new genus, gen. nov., sp. nov. The type strain of is PRI-4131 ( = DSM 25763  = ATCC BAA-2444).

Funding
This study was supported by the:
  • Technology Development Fund, Icelandic Centre for Research
  • AVS R&D Fund, Iceland Ministry of Fisheries and Agriculture
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.044115-0
2013-03-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/3/1149.html?itemId=/content/journal/ijsem/10.1099/ijs.0.044115-0&mimeType=html&fmt=ahah

References

  1. Alfredsson G. A., Kristjansson J. K., Hjorleifsdottir S., Stetter K. O. ( 1988 ). Rhodothermus marinus, gen., nov., sp. nov., a thermophilic, halophilic bacterium from submarine hot springs in Iceland. . J Gen Microbiol 134, 299306. [View Article]
    [Google Scholar]
  2. Aziz R. K., Bartels D., Best A. A., DeJongh M., Disz T., Edwards R. A., Formsma K., Gerdes S., Glass E. M. & other authors ( 2008 ). The RAST Server: rapid annotations using subsystems technology. . BMC Genomics 9, 75. [View Article] [PubMed]
    [Google Scholar]
  3. Bjornsdottir S. H., Petursdottir S. K., Hreggvidsson G. O., Skirnisdottir S., Hjorleifsdottir S., Arnfinnsson J., Kristjansson J. K. ( 2009 ). Thermus islandicus sp. nov., a mixotrophic sulfur-oxidizing bacterium isolated from the Torfajokull geothermal area. . Int J Syst Evol Microbiol 59, 29622966. [View Article] [PubMed]
    [Google Scholar]
  4. Blazejak A., Schippers A. ( 2010 ). High abundance of JS-1- and Chloroflexi-related bacteria in deeply buried marine sediments revealed by quantitative, real-time PCR. . FEMS Microbiol Ecol 72, 198207. [View Article] [PubMed]
    [Google Scholar]
  5. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. ( 1977 ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81, 461466. [View Article] [PubMed]
    [Google Scholar]
  6. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  7. Felsenstein J. ( 2009 ). phylip (phylogeny inference package), version 3.69. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  8. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  9. Grégoire P., Bohli M., Cayol J. L., Joseph M., Guasco S., Dubourg K., Cambar J., Michotey V., Bonin P. & other authors ( 2011 ). Caldilinea tarbellica sp. nov., a filamentous, thermophilic, anaerobic bacterium isolated from a deep hot aquifer in the Aquitaine Basin. . Int J Syst Evol Microbiol 61, 14361441. [View Article] [PubMed]
    [Google Scholar]
  10. Hanada S., Hiraishi A., Shimada K., Matsuura K. ( 1995 ). Isolation of Chloroflexus aurantiacus and related thermophilic phototrophic bacteria from Japanese hot springs using an improved isolation procedure. . J Gen Appl Microbiol 41, 119130. [View Article]
    [Google Scholar]
  11. Hanada S., Takaichi S., Matsuura K., Nakamura K. ( 2002 ). Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. . Int J Syst Evol Microbiol 52, 187193.[PubMed] [CrossRef]
    [Google Scholar]
  12. Hjorleifsdottir S., Skirnisdottir S., Hreggvidsson G. O., Holst O., Kristjansson J. K. ( 2001 ). Species compostion of cultivated and non-cultivated bacteria from short filaments in an Icelandic hot spring at 88°C. . Microb Ecol 42, 117125.[PubMed]
    [Google Scholar]
  13. Hobel C. F. V., Marteinsson V. T., Hreggvidsson G. O., Kristjánsson J. K. ( 2005 ). Investigation of the microbial ecology of intertidal hot springs by using diversity analysis of 16S rRNA and chitinase genes. . Appl Environ Microbiol 71, 27712776. [View Article] [PubMed]
    [Google Scholar]
  14. Hugenholtz P., Stackebrandt E. ( 2004 ). Reclassification of Sphaerobacter thermophilus from the subclass Sphaerobacteridae in the phylum Actinobacteria to the class Thermomicrobia (emended description) in the phylum Chloroflexi (emended description). . Int J Syst Evol Microbiol 54, 20492051. [View Article] [PubMed]
    [Google Scholar]
  15. Hugenholtz P., Goebel B. M., Pace N. R. ( 1998 ). Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. . J Bacteriol 180, 47654774.[PubMed]
    [Google Scholar]
  16. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 211232. Edited by Munro H. N. . New York:: Academic Press;.
    [Google Scholar]
  17. Kurtzman C. P., Baker F. L., Smiley M. J. ( 1974 ). Specimen holder to critical-point dry microorganisms for scanning electron microscopy. . Appl Microbiol 28, 708712.[PubMed]
    [Google Scholar]
  18. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E. ( 1988 ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38, 358361. [View Article]
    [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  20. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  21. Sekiguchi Y., Yamada T., Hanada S., Ohashi A., Harada H., Kamagata Y. ( 2003 ). Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level. . Int J Syst Evol Microbiol 53, 18431851. [View Article] [PubMed]
    [Google Scholar]
  22. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  23. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  24. Yabe S., Aiba Y., Sakai Y., Hazaka M., Yokota A. ( 2010 ). Thermosporothrix hazakensis gen. nov., sp. nov., isolated from compost, description of Thermosporotrichaceae fam. nov. within the class Ktedonobacteria Cavaletti et al. 2007 and emended description of the class Ktedonobacteria . . Int J Syst Evol Microbiol 60, 17941801. [View Article] [PubMed]
    [Google Scholar]
  25. Yamada T., Sekiguchi Y., Hanada S., Imachi H., Ohashi A., Harada H., Kamagata Y. ( 2006 ). Anaerolinea thermolimosa sp. nov., Levilinea saccharolytica gen. nov., sp. nov., and Leptolinea tardivitalis gen. nov., sp., nov., novel filamentous anaerobes, and description of the new classes Anaerolinea classis nov., and Caldilineae classis nov. in the bacterial phylum Chloroflexi . . Int J Syst Evol Microbiol 56, 13311340. [View Article] [PubMed]
    [Google Scholar]
  26. Yamada T., Imachi H., Ohashi A., Harada H., Hanada S., Kamagata Y., Sekiguchi Y. ( 2007 ). Bellilinea caldifistulae gen. nov., sp. nov., and Longilinea arvoryzae gen. nov., sp. nov., strictly anaerobic, filamentous bacteria of the phylum Chloroflexi isolated from methanogenic propionate-degrading consortia. . Int J Syst Evol Microbiol 57, 22992306. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.044115-0
Loading
/content/journal/ijsem/10.1099/ijs.0.044115-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error