1887

Abstract

A marine bacterial strain, designated MD2, was isolated from the damaged tissue of a hydrocoral, , collected from the coral reef in the northern Red Sea, Gulf of Eilat, Israel. Strain MD2 was Gram-reaction-negative, rod-shaped and motile, and formed small, creamy and opaque colonies, 1–2 mm in diameter, after 3 days incubation on Marine agar at 30°C. The novel strain grew well in nutrient broth at 1.5–6 % NaCl and at 20–37°C. The major cellular fatty acids were iso-Cω9, iso-C, Cω7 and Cω6. The polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, an unidentified lipid, two unidentified phospholipids, two unidentified glycolipids and two unidentified aminolipids. Ubiquinone Q-10 was the only respiratory lipoquinone. The DNA G+C content was 60.3 mol%. Analysis of the 16S rRNA gene sequence placed the organism in the α-subclass of the with a sequence divergence of about 9 % from any species with a validly published name. The highest 16S rRNA gene sequence similarity (approximately 91 %) was notably with type strains of members of the genus , 101-1 , S3-22 and GW14-5. On the basis of genotypic, chemotaxonomic and phenotypic distinctness, strain MD2 represents a novel species in a new genus of the class , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is MD2 ( = LMG 26586 = DSM 25217).

Funding
This study was supported by the:
  • , ISF , (Award 1169/07)
  • , VATAT
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2013-05-01
2021-02-25
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References

  1. Bazylinski D. A., Williams T. J., Lefèvre C. T., Berg R. J., Zhang C. L., Bowser S. S., Dean A. J., Beveridge T. J. ( 2013 ). Magnetococcus marinus gen. nov., sp. nov., a marine, magnetotactic bacterium that represents a novel lineage (Magnetococcaceae fam. nov., Magnetococcales ord. nov.) at the base of the Alphaproteobacteria . . Int J Syst Evol Microbiol 63, 801808. [CrossRef] [PubMed]
    [Google Scholar]
  2. Ben-Haim Y., Banim E., Kushmaro A., Loya Y., Rosenberg E. ( 1999 ). Inhibition of photosynthesis and bleaching of zooxanthellae by the coral pathogen Vibrio shiloi . . Environ Microbiol 1, 223229. [CrossRef] [PubMed]
    [Google Scholar]
  3. Cho J. C., Giovannoni S. J. ( 2003 ). Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the alpha-Proteobacteria . . Int J Syst Evol Microbiol 53, 10311036. [CrossRef] [PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  5. Fredricks D. N. ( 2006 ). Introduction to the Rickettsiales and other intracellular prokaryotes. . In The Prokaryotes. A Handbook on the Biology of Bacteria, Part 1, vol. 5, pp. 457466. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York:: Springer;. [CrossRef]
    [Google Scholar]
  6. Garrity G. M., Holt J. G. ( 2001 ). The road map to the Manual . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 1, pp. 119166. Edited by Boone D. R., Castenholz R. W., Garrity G. M. . New York:: Springer;. [CrossRef]
    [Google Scholar]
  7. Giovannoni S. J., Tripp H. J., Givan S., Podar M., Vergin K. L., Baptista D., Bibbs L., Eads J., Richardson T. H. & other authors ( 2005 ). Genome streamlining in a cosmopolitan oceanic bacterium. . Science 309, 12421245. [CrossRef] [PubMed]
    [Google Scholar]
  8. Jordan E. M., Thompson F. L., Zhang X. H., Li Y., Vancanneyt M., Kroppenstedt R. M., Priest F. G., Austin B. ( 2007 ). Sneathiella chinensis gen. nov., sp. nov., a novel marine alphaproteobacterium isolated from coastal sediment in Qingdao, China. . Int J Syst Evol Microbiol 57, 114121. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kersters K., De Vos P., Gillis M., Swings J., Vandamme P., Stackebrandt E. ( 2006 ). Introduction to the Proteobacteria. . In The Prokaryotes. A Handbook on the Biology of Bacteria, Part 1, vol. 5, pp. 337. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York:: Springer;.
    [Google Scholar]
  10. Kwon K. K., Lee H. S., Yang S. H., Kim S. J. ( 2005 ). Kordiimonas gwangyangensis gen. nov., sp. nov., a marine bacterium isolated from marine sediments that forms a distinct phyletic lineage (Kordiimonadales ord. nov.) in the ‘Alphaproteobacteria’. . Int J Syst Evol Microbiol 55, 20332037. [CrossRef] [PubMed]
    [Google Scholar]
  11. Math R. K., Jeong S. H., Jin H. M., Park M. S., Kim J. M., Jeon C. O. ( 2012 ). Kordiimonas aestuarii sp. nov., a marine bacterium isolated from a tidal flat. . Int J Syst Evol Microbiol 62, 30493054. [CrossRef] [PubMed]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  13. Paramasivam N., Ben-Dov E., Arotsker L., Kramarsky-Winter E., Zvuloni A., Loya Y., Kushmaro A. ( 2013 ). Bacterial consortium of Millepora dichotoma exhibiting unusual multifocal lesion event in the Gulf of Eilat, Red Sea. . Microb Ecol 65, 5059. [CrossRef] [PubMed]
    [Google Scholar]
  14. Rasoulouniriana D., Siboni N., Ben-Dov E., Kramarsky-Winter E., Loya Y., Kushmaro A. ( 2009 ). Pseudoscillatoria coralii gen. nov., sp. nov., a cyanobacterium associated with coral black band disease (BBD). . Dis Aquat Organ 87, 9196. [CrossRef] [PubMed]
    [Google Scholar]
  15. Rosenberg E., Loya Y. (editors) ( 2004 ). Coral Health and Disease. Berlin:: Springer;. [CrossRef]
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  17. Skorpil P., Broughton W. J. ( 2006 ). Molecular interactions between Rhizobium and legumes. . Prog Mol Subcell Biol 41, 143164. [CrossRef] [PubMed]
    [Google Scholar]
  18. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4 . 0. . Mol Biol Evol 24, 15961599. [CrossRef] [PubMed]
    [Google Scholar]
  19. Wiese J., Thiel V., Gärtner A., Schmaljohann R., Imhoff J. F. ( 2009 ). Kiloniella laminariae gen. nov., sp. nov., an alphaproteobacterium from the marine macroalga Laminaria saccharina . . Int J Syst Evol Microbiol 59, 350356. [CrossRef] [PubMed]
    [Google Scholar]
  20. Wilson K. ( 1987 ). Preparation of genomic DNA from bacteria. . In Current Protocols in Molecular Biology, pp. 241245. Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. . New York:: Greene Publishing and Wiley-Interscience;.
    [Google Scholar]
  21. Xu X. W., Huo Y. Y., Bai X. D., Wang C. S., Oren A., Li S. Y., Wu M. ( 2011 ). Kordiimonas lacus sp. nov., isolated from a ballast water tank, and emended description of the genus Kordiimonas . . Int J Syst Evol Microbiol 61, 422426. [CrossRef] [PubMed]
    [Google Scholar]
  22. Zvuloni A., Armoza-Zvuloni R., Shaked Y. ( 2011 ). Multifocal bleaching in Millepora dichotoma at the Gulf of Aqaba (Eilat), Red Sea. . Mar Ecol Prog Ser 441, 2532. [CrossRef]
    [Google Scholar]
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