1887

Abstract

A psychrophilic, Gram-negative bacterium, designated MOL-1, was isolated from water of Lake Michigan. 16S rRNA gene sequence analysis revealed that the sequence of strain MOL-1 has sequence similarity of 95.6, 94.8, 94.3, 94.3, 94.2 and 93.9 %, respectively, to the 16S rRNA gene sequences of HS39, WCC 4512, T5-12, TR6-04, IBFC2009 and WCC 4521. The major cellular fatty acids were iso-C, iso-C 3-OH and summed feature 3 (iso-C 2-OH and/or Cω7). Menaquinone MK-7 is the predominant respiratory quinone, while -homospermidine is the predominant polyamine. The polar lipid profile is composed of the predominant lipids phosphatidylethanolamine and unidentified polar lipid L2, with moderate amounts of unidentified polar lipids L1, L5 and L6 and unidentified aminophospholipids APL1 and APL2 and minor to trace amounts of unidentified polar lipids L3, L4, L7, L8, L9 and L10, unidentified phospholipid PL4 and unidentified aminophospholipid APL3. After molecular and phenotypic studies, including chemotaxonomic analyses, it was concluded that strain MOL-1 represents a novel species, for which the name sp. nov. is proposed. The type strain is MOL-1 ( = NRRL B-59232  = DSM 22418).

Funding
This study was supported by the:
  • , Spanish Ministry of Economy and Competitivity
  • , Consolider Ingenio , (Award CLG2009_12651-C02-02, CLG2009_12651-C02-01 and 2010CE-CSD2007-0005)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.043844-0
2013-03-01
2020-11-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/3/952.html?itemId=/content/journal/ijsem/10.1099/ijs.0.043844-0&mimeType=html&fmt=ahah

References

  1. Albert R. A., Archambault J., Rosselló-Mora R., Tindall B. J., Matheny M. ( 2005 ). Bacillus acidicola sp. nov., a novel mesophilic, acidophilic Bacillus species isolated from acidic Sphagnum peat bogs in Wisconsin. . Int J Syst Evol Microbiol 55, 21252130. [CrossRef] [PubMed]
    [Google Scholar]
  2. Albert R. A., Waas N. E., Langer S., Pavlons S. C., Feldner J. L., Rosselló-Mora R., Busse H.-J. ( 2010 ). Labrys wisconsinensis sp. nov., a budding bacterium isolated from Lake Michigan water, and emended description of the genus Labrys . . Int J Syst Evol Microbiol 60, 15701576. [CrossRef] [PubMed]
    [Google Scholar]
  3. Allgaier M., Grossart H. P. ( 2006 ). Diversity and seasonal dynamics of Actinobacteria populations in four lakes in northeastern Germany. . Appl Environ Microbiol 72, 34893497. [CrossRef] [PubMed]
    [Google Scholar]
  4. Altenburger P., Busse H.-J., Kämpfer P., Lubitz W., Makristathis A. ( 1996 ). Classification of bacteria isolated from a medieval wall painting. . J Biotechnol 47, 3952. [CrossRef]
    [Google Scholar]
  5. Busse H.-J., Auling G. ( 1988 ). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . . Syst Appl Microbiol 11, 18. [CrossRef]
    [Google Scholar]
  6. Choi H.-A., Lee S.-S. ( 2012 ). Sphingobacterium kyonggiense sp. nov., isolated from chloroethene-contaminated soil, and emended descriptions of Sphingobacterium daejeonense and Sphingobacterium mizutaii . . Int J Syst Evol Microbiol 62, 25592564. [CrossRef] [PubMed]
    [Google Scholar]
  7. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 22592261. [CrossRef] [PubMed]
    [Google Scholar]
  8. Cottrell M. T., Waidner L. A., Yu L. Y., Kirchman D. L. ( 2005 ). Bacterial diversity of metagenomic and PCR libraries from the Delaware River. . Environ Microbiol 7, 18831895. [CrossRef] [PubMed]
    [Google Scholar]
  9. Duan S., Liu Z., Feng X., Zheng K., Cheng L. ( 2009 ). Sphingobacterium bambusae sp. nov., isolated from soil of bamboo plantation. . J Microbiol 47, 693698. [CrossRef] [PubMed]
    [Google Scholar]
  10. Eiler A., Bertilsson S. ( 2004 ). Composition of freshwater bacterial communities associated with cyanobacterial blooms in four Swedish lakes. . Environ Microbiol 6, 12281243. [CrossRef] [PubMed]
    [Google Scholar]
  11. Gordon R. E., Haynes W., Pang C. H.-N. ( 1973 ). The Genus Bacillus. Washington, DC:: US Department of Agriculture;.
    [Google Scholar]
  12. Hamana K., Matsuzaki S. ( 1991 ). Polyamine distributions in the Flavobacterium-Cytophaga-Sphingobacterium complex. . Can J Microbiol 37, 885888. [CrossRef]
    [Google Scholar]
  13. He X., Xiao T., Kuang H., Lan X., Tudahong M., Osman G., Fang C., Rahman E. ( 2010 ). Sphingobacterium shayense sp. nov., isolated from forest soil. . Int J Syst Evol Microbiol 60, 23772381. [CrossRef] [PubMed]
    [Google Scholar]
  14. Islam M. S., Kawasaki H., Nakagawa Y., Hattori T., Seki T. ( 2007 ). Labrys okinawensis sp. nov. and Labrys miyagiensis sp. nov., budding bacteria isolated from rhizosphere habitats in Japan, and emended descriptions of the genus Labrys and Labrys monachus . . Int J Syst Evol Microbiol 57, 552557. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kim K.-H., Ten L. N., Liu Q. M., Im W.-T., Lee S.-T. ( 2006 ). Sphingobacterium daejeonense sp. nov., isolated from a compost sample. . Int J Syst Evol Microbiol 56, 20312036. [CrossRef] [PubMed]
    [Google Scholar]
  16. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [CrossRef] [PubMed]
    [Google Scholar]
  17. Maturin L., Peeler J. T. ( 2001 ). Aerobic plate count. . In Bacteriological Analytical Manual, chapter 3. Edited by Merker R. I., Jackson G. J., Bandler R. . Silver Spring, MD:: US Food and Drug Administration;. http://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063346.htm
    [Google Scholar]
  18. Müller-Spitz S. R., Goetz G. W., McLellan S. L. ( 2009 ). Temporal and spatial variability in nearshore bacterioplankton communities of Lake Michigan. . FEMS Microbiol Ecol 67, 511522. [CrossRef] [PubMed]
    [Google Scholar]
  19. Murray R. G. E., Doetsch R. N., Robinow C. F. ( 1994 ). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 2141. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  20. Newton R. J., Kent A. D., Triplett E. W., McMahon K. D. ( 2006 ). Microbial community dynamics in a humic lake: differential persistence of common freshwater phylotypes. . Environ Microbiol 8, 956970. [CrossRef] [PubMed]
    [Google Scholar]
  21. Santo Domingo J. W., Meckes M. C., Simpson J. M., Sloss B., Reasoner D. J. ( 2003 ). Molecular characterization of bacteria inhabiting a water distribution system simulator. . Water Sci Technol 47 (5), 149154.[PubMed]
    [Google Scholar]
  22. Schauer M., Kamenik C., Hahn M. W. ( 2005 ). Ecological differentiation within a cosmopolitan group of planktonic freshwater bacteria (SOL cluster, Saprospiraceae, Bacteroidetes). . Appl Environ Microbiol 71, 59005907. [CrossRef] [PubMed]
    [Google Scholar]
  23. Schmidt V. S. J., Wenning M., Scherer S. ( 2012 ). Sphingobacterium lactis sp. nov. and Sphingobacterium alimentarium sp. nov., isolated from raw milk and a dairy environment. . Int J Syst Evol Microbiol 62, 15061511. [CrossRef] [PubMed]
    [Google Scholar]
  24. Smibert R. M., Krieg R. N. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  25. Stamatakis A. ( 2006 ). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22, 26882690. [CrossRef] [PubMed]
    [Google Scholar]
  26. Stolz A., Busse H.-J., Kämpfer P. ( 2007 ). Pseudomonas knackmussii sp. nov.. Int J Syst Evol Microbiol 57, 572576. [CrossRef] [PubMed]
    [Google Scholar]
  27. Ten L. N., Liu Q.-M., Im W.-T., Aslam Z., Lee S.-T. ( 2006 ). Sphingobacterium composti sp. nov., a novel DNase-producing bacterium isolated from compost. . J Microbiol Biotechnol 16, 17281733.
    [Google Scholar]
  28. Tindall B. J. ( 1990a ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128130. [CrossRef]
    [Google Scholar]
  29. Tindall B. J. ( 1990b ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [CrossRef]
    [Google Scholar]
  30. Van der Gucht K., Vandekerckhove T., Vloemans N., Cousin S., Muylaert K., Sabbe K., Gillis M., Declerk S., De Meester L., Vyverman W. ( 2005 ). Characterization of bacterial communities in four freshwater lakes differing in nutrient load and food web structure. . FEMS Microbiol Ecol 53, 205220. [CrossRef] [PubMed]
    [Google Scholar]
  31. Williams M. M., Domingo J. W., Meckes M. C., Kelty C. A., Rochon H. S. ( 2004 ). Phylogenetic diversity of drinking water bacteria in a distribution system simulator. . J Appl Microbiol 96, 954964. [CrossRef] [PubMed]
    [Google Scholar]
  32. Yabuuchi E., Tanimura E., Ohyama A., Yano I., Yamamoto A. ( 1979 ). Flavobacterium devorans ATCC 10829: a strain of Pseudomonas paucimobilis . . J Gen Appl Microbiol 25, 95107. [CrossRef]
    [Google Scholar]
  33. Yabuuchi E., Kaneko T., Yano I., Moss C. W., Miyoshi N. ( 1983 ). Sphingobacterium gen. nov., Sphingobacterium spiritivorum comb. nov., Sphingobacterium multivorum comb. nov., Sphingobacterium mizutae sp. nov., and Flavobacterium indologenes sp. nov.: glucose-nonfermenting gram-negative rods in CDC groups IIK-2 and IIb. . Int J Syst Bacteriol 33, 580598. [CrossRef]
    [Google Scholar]
  34. Yamaguchi S., Yokoe M. ( 2000 ). A novel protein deaminating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. . Appl Environ Microbiol 66, 33373343. [CrossRef] [PubMed]
    [Google Scholar]
  35. Yano I., Tomiyasu I., Yabuuchi E. ( 1982 ). Long chain base composition of strains of three species of Sphingobacterium gen. nov.. FEMS Microbiol Lett 15, 303307. [CrossRef]
    [Google Scholar]
  36. Yarza P., Richter M., Peplies J., Euzéby J., Amann R., Schleifer K.-H., Ludwig W., Glöckner F. O., Rosselló-Móra R. ( 2008 ). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. . Syst Appl Microbiol 31, 241250. [CrossRef] [PubMed]
    [Google Scholar]
  37. Zwart G., Crump B. C., Kamst-van Agterveld M. P., Hagen F., Han S. K. ( 2002 ). Typical freshwater bacteria: an analysis of available 16S rRNA gene sequences from plankton of lakes and rivers. . Aquat Microb Ecol 28, 141155. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.043844-0
Loading
/content/journal/ijsem/10.1099/ijs.0.043844-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error