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Abstract

Bacterial strains CNX-216 and CNU-914 were isolated from marine sediment samples collected from Palmyra Atoll and off Catalina Island, respectively. Both strains were Gram-negative and aerobic and produce deep-orange to pink colonies and alkaloid secondary metabolites. Cells of strain CNX-216 were short, non-motile rods, whereas cells of strain CNU-914 were short, curved rods with gliding motility. The DNA G+C contents of CNX-216 and CNU-914 were respectively 57.7 and 44.4 mol%. Strains CNX-216 and CNU-914 contained MK-7 as the predominant menaquinone and iso-C and Cω5 as the major fatty acids. Phylogenetic analyses revealed that both strains belong to the order in the phylum . Strain CNX-216 exhibited low 16S rRNA gene sequence identity (87.1 %) to the nearest type strain, 311, and formed a well-supported lineage that is outside all currently described families in the order . Strain CNU-914 shared 97.6 % 16S rRNA gene sequence identity with ‘’ N5EA6-3A2B and, together with ‘’ N5DB8-4 and four uncharacterized marine bacteria isolated as part of this study, formed a lineage that is clearly distinguished from other families in the order . Based on our polyphasic taxonomic characterization, we propose that strains CNX-216 and CNU-914 represent novel genera and species, for which we propose the names gen. nov., sp. nov. (type strain CNX-216  = DSM 25187  = KCCM 90102) and gen. nov., sp. nov. (type strain CNU-914  = DSM 25186  = KCCM 90101) within the new families fam. nov. and fam. nov.

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2013-04-01
2020-01-22
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