sp. nov., a slightly thermophilic betaproteobacterium isolated from a hot spring Free

Abstract

A slightly thermophilic bacterial strain, designated AT-A2, was isolated from a hot spring water sample taken from the Antun hot spring in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain AT-A2 were aerobic, Gram-negative, motile by a single polar flagellum and formed non-pigmented colonies. Growth occurred at 35–60 °C (optimum, 55 °C), with 0–1.0 % NaCl (optimum, 0.2 %) and at pH 7.0–9.0 (optimum, pH 7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain AT-A2 belonged to the genus and its closest neighbour was AA-1 with a sequence similarity of 97.5 %. The predominant cellular fatty acids were C (40.2 %), summed feature 3 (Cω7 and/or Cω6; 20.1 %) and C cyclo (11.5 %). The major respiratory quinone was Q-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminolipid and several uncharacterized phospholipids. The DNA G+C content of strain AT-A2 was 70.1 mol%. The mean level of DNA–DNA relatedness between strain AT-A2 and AA-1 was 23.9 %. On the basis of the phylogenetic and phenotypic data, strain AT-A2 should be classified as representing a novel species, for which the name sp. nov. is proposed. The type strain is AT-A2 ( = LMG 26746 = KCTC 23862 = BCRC 80391).

Funding
This study was supported by the:
  • Bureau of Energy, Ministry of Economic Affairs of Taiwan (Award 101-D0112)
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2013-05-01
2024-03-28
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